3 results about "Transgenic Rats" patented technology

A genetically-modified rat having a PKHD1L1 gene with a point or other mutation and a construction method thereof are disclosed. A CRISPR / Cas9 system knocks the PKHD1L1 gene into a rat source, and a codon changes from TTA to TCA to construct the mutant PKHD1L1 gene. The genetically-modified rat can be applied to epilepsy pathogenesis studies and design and testing of new anti-epileptic drugs. Methods for detecting abnormal cortical excitability and detecting an epileptic phenotype of an animal can use the genetically-modified rat. A somatosensory evoked potential is used to detect whether the genetically-modified rat has an abnormal cortical excitability phenotype, so as to confirm whether the rat can be a successful model for testing anti-epileptic drugs. The method can detect abnormal cortical excitability and verify the effectiveness of anti-epileptic drugs or treatments.

A genetically-modified rat having a PKHD1L1 gene with a point or other mutation and a construction method thereof are disclosed. A CRISPR / Cas9 system knocks the PKHD1L1 gene into a rat source, and a codon changes from TTA to TCA to construct the mutant PKHD1L1 gene. The genetically-modified rat can be applied to epilepsy pathogenesis studies and design and testing of new anti-epileptic drugs. Methods for detecting abnormal cortical excitability and detecting an epileptic phenotype of an animal can use the genetically-modified rat. A somatosensory evoked potential is used to detect whether the genetically-modified rat has an abnormal cortical excitability phenotype, so as to confirm whether the rat can be a successful model for testing anti-epileptic drugs. The method can detect abnormal cortical excitability and verify the effectiveness of anti-epileptic drugs or treatments.

The purpose of the present invention is to provide a method for thawing cryopreserved rat eggs. The present invention provides a method for thawing cryopreserved rat eggs, the method being characterized in that the thawing is carried out in a thawing solution containing 0.05 M to 0.2 M of sucrose. The present invention also provides a method for producing pups from cryopreserved fertilized rat eggs, the method comprising: a step for thawing the cryopreserved fertilized rat eggs in a thawing solution containing about 0.05 M to about 0.2 M of sucrose and culturing the eggs; a step for selecting and culturing morphologically normal eggs if necessary; optionally, a step for genetically engineering the cultured fertilized eggs to create genetically modified fertilized eggs and culturing the eggs; and a step for transplanting the fertilized rat eggs into a recipient female rat to generate the pups.