4 results about "Transgenic Rats" patented technology

A genetically-modified rat having a PKHD1L1 gene with a point or other mutation and a construction method thereof are disclosed. A CRISPR / Cas9 system knocks the PKHD1L1 gene into a rat source, and a codon changes from TTA to TCA to construct the mutant PKHD1L1 gene. The genetically-modified rat can be applied to epilepsy pathogenesis studies and design and testing of new anti-epileptic drugs. Methods for detecting abnormal cortical excitability and detecting an epileptic phenotype of an animal can use the genetically-modified rat. A somatosensory evoked potential is used to detect whether the genetically-modified rat has an abnormal cortical excitability phenotype, so as to confirm whether the rat can be a successful model for testing anti-epileptic drugs. The method can detect abnormal cortical excitability and verify the effectiveness of anti-epileptic drugs or treatments.

A genetically-modified rat having a PKHD1L1 gene with a point or other mutation and a construction method thereof are disclosed. A CRISPR / Cas9 system knocks the PKHD1L1 gene into a rat source, and a codon changes from TTA to TCA to construct the mutant PKHD1L1 gene. The genetically-modified rat can be applied to epilepsy pathogenesis studies and design and testing of new anti-epileptic drugs. Methods for detecting abnormal cortical excitability and detecting an epileptic phenotype of an animal can use the genetically-modified rat. A somatosensory evoked potential is used to detect whether the genetically-modified rat has an abnormal cortical excitability phenotype, so as to confirm whether the rat can be a successful model for testing anti-epileptic drugs. The method can detect abnormal cortical excitability and verify the effectiveness of anti-epileptic drugs or treatments.

The invention discloses a supramolecular fluorescent sensing array and application thereof in preparation of a preparation or a kit for early diagnosis of Alzheimer's disease, the supramolecular fluorescent sensing array comprises a plurality of sensing units, and each sensing unit is composed of a macrocyclic main solution and a fluorescent indicator solution; a macrocyclic main body used in the macrocyclic main body solution is selected from at least one of any macrocyclic main bodies; a fluorescent indicator used in the fluorescent indicator solution is selected from at least one of any fluorescent indicators. The supramolecular fluorescence sensing array can accurately distinguish cerebrospinal fluid samples of alzheimer disease transgenic rats in different disease course stages, and a new method is provided for early-stage accurate diagnosis and disease course monitoring of the alzheimer disease.

The purpose of the present invention is to provide a method for thawing cryopreserved rat eggs. The present invention provides a method for thawing cryopreserved rat eggs, the method being characterized in that the thawing is carried out in a thawing solution containing 0.05 M to 0.2 M of sucrose. The present invention also provides a method for producing pups from cryopreserved fertilized rat eggs, the method comprising: a step for thawing the cryopreserved fertilized rat eggs in a thawing solution containing about 0.05 M to about 0.2 M of sucrose and culturing the eggs; a step for selecting and culturing morphologically normal eggs if necessary; optionally, a step for genetically engineering the cultured fertilized eggs to create genetically modified fertilized eggs and culturing the eggs; and a step for transplanting the fertilized rat eggs into a recipient female rat to generate the pups.