Recessive gene xa13 of rice bacterial blight resistance and its allelic dominant gene xa13

A dominant gene and recessive gene technology, applied in the field of plant biology, can solve the problems of reduced germination rate and crispy rice.

Inactive Publication Date: 2006-11-15
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bacterial blight causes crisp rice quality and reduced germination rate

Method used

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  • Recessive gene xa13 of rice bacterial blight resistance and its allelic dominant gene xa13
  • Recessive gene xa13 of rice bacterial blight resistance and its allelic dominant gene xa13
  • Recessive gene xa13 of rice bacterial blight resistance and its allelic dominant gene xa13

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Construction of a physical map of the xa13 segment of the recessive gene resistant to bacterial blight

[0026] 1. Experimental materials and inoculation methods

[0027] The indica rice line "IRBB13" carrying the recessive gene xa13 and the near-isogenic line of "IRBB13" (recurrent susceptible parent), "IR24" carrying the dominant gene Xa13 (Chu et al., 2004, Genome-wide analysis ofdefense-responsive genes in bacterial blight resistance of rice mediated by the recessive R genexa13.Mol.Genet.Genomics 271:111-120) constructed three F 2 group. The first population consisted of 250 extremely resistant (lesion length less than 3 cm two weeks after inoculation with bacterial blight PXO99) individual plants, the second population consisted of 6000 random individual plants, and the third population consisted of 1972 individual plants Composition of random individual plants. Use the three F's 2 Population mapping of the recessive gene xa13.

[0028] The present ...

Embodiment 2

[0036] Example 2: Fine-mapping the recessive gene xa13

[0037] Molecular marker E6a in the second F 2 Recombination with the xa13 site was detected in 26 individual plants of the population ( figure 2 ). The 26 recombinant exchange individuals detected with the E6a marker were further analyzed with a series of molecular markers from the sequenced BAC clones. These markers include 6 shotgun clones (RP3, RP4, RP5, RP7, RP8 and RP10) derived from BAC clones 21H14 and 14L03, the SSR (simple sequence repeat) marker SR6 designed based on the 21H14 sequence and the CAPS marker ST9 designed based on the 14L03 sequence . The analysis found that the markers RP3, RP4, RP5, SR6 and RP7 were detected to recombine with the xa13 locus in 1 to 15 of the 26 individual plants related to the E6a marker, while the markers RP8, ST9 and RP10 were detected in the 26 individual plants. All of the individual plants were co-segregated with the xa13 locus ( figure 2 ).

[0038] The SSR marker S...

Embodiment 3

[0039] Example 3: Isolation and functional verification of the dominant gene Xa13

[0040] 1. Determination of Xa13 candidate genes

[0041] Using GenScan ( http: / / genes.mit.edu / GENSCAN.html ) and Fgenesh (http: / / www.softberry.com / ) gene prediction software for molecular markers RP7 and ST9, including xa13 allele dominant gene Xa13, derived from rice variety IR64 14.8kb ( figure 2 ) sequence analysis, it was shown that there may be three genes in this segment. The first and third genes are incomplete and the second gene is complete. Therefore, it is speculated that the second gene is the dominant gene Xa13.

[0042] 2. Isolation and verification of Xa13 candidate genes

[0043] The present invention uses restriction endonuclease BamHI to digest the plasmid DNA of the BAC clone 14L03 of rice variety IR64, and isolates a candidate gene comprising the dominant gene Xa13, a DNA fragment about 14 kb in length ( image 3 ). The Agrobacterium-mediated genetic transformation v...

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Abstract

This invention relates to the plant biological technology area, it concretely relates to the separated clone, function testing and application of the two DNA fragments of the bacterial blight of rice recessive gene xa13 and dominant allele gene xa13. The antiviral allele xa13 gene is the function flow mutant of the dominant gene xa13. The mutant of the dominant gene makes the paddy rice generated the resistance of the bacterial blight. Some DNA fragments of the allele and dominant gene are connected with the extraneous regulated sequence and transferred into the paddy rice by the gene transfer technology bases on the RNA interference, and this can resist the function of the dominant gene in the paddy rice and improve the resistance of the bacterial blight of rice. Otherwise, the allele gene xa13 in the paddy rice is suppressed by the RNA technology, and this can improve the resistance of bacterial blight of rice further.

Description

technical field [0001] The invention relates to the field of plant biotechnology. It specifically relates to the isolation, cloning, functional verification and application of a rice bacterial blight-resistant recessive gene xa13. The disease resistance recessive gene xa13 is a functionally deficient mutant of its allelic (dominant) gene Xa13. The mutation of the dominant gene Xa13 confers resistance to some bacterial blight in rice. Background technique [0002] Plants are attacked by various pathogens during their growth. There are many types of plant pathogens, including viruses, bacteria, molds and nematodes. Pathogen invasion of plants leads to two results: (1) the pathogen successfully reproduces in the host plant, causing related diseases; (2) the host plant produces a disease-resistant response, killing the pathogen or preventing its growth. Using resistance gene resources to improve plant disease resistance is the fundamental way to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82
Inventor 王石平储昭晖袁猛葛小佳杨红
Owner HUAZHONG AGRI UNIV
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