Rape heat shock protein gene HSP17.8 and application thereof

Abstract

The invention discloses a rape heat shock protein gene HSP17.8 and application thereof. The invention provides a nucleotide sequence and an amino acid sequence of the gene, gene sequences which at least have 70 percent homology with a DNA sequence shown in SEQ ID NO:1 in other crops, and mutant alleles or derivatives produced by adding, substituting, inserting or deleting one or more nucleotides. At the same time, the heat shock protein gene also proves that the overexpression of the heat shock protein gene strengthens the heat resistance of arabidopsis at high temperature by means of model plant, namely the arabidopsis through transgenic technology, increases the yield of seeds at high temperature, and improves the yield of the arabidopsis under high temperature stress, so the heat shock protein gene has good application prospect in heat-tolerance breeding of crops.

Description

technical field [0001] The present invention belongs to the field of molecular biology. Specifically, the present invention relates to a rapeseed heat shock protein gene HSP17.8. The present invention provides the nucleic acid sequence and protein sequence of the gene, and also relates to the use of the gene in heat-resistant breeding of crops. The overexpression of rapeseed heat shock gene HSP17.8 in Arabidopsis shows that this gene can significantly enhance the heat tolerance of plants and provide a guarantee for increasing crop yield under high temperature stress. Background technique [0002] Plants are often stressed by various abiotic factors during their growth and development, among which temperature is the main factor affecting plant growth. With the acceleration of industrialization, the global ecological environment has deteriorated, and the greenhouse effect has led to an increase in global temperature. The global average temperature has risen by 0.6°C in the 20...

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Problems solved by technology

In addition, although the accumulation of sHSP during heat shock can protect cells from damage, the overexpression of inducible sHSP at room temperature does not necessarily improve the heat resistance at high temperature, and the transgenic lines overexpressing HSP21 are only under strong light conditions. In order to improve its heat tolerance, Sun et al. found that the overexpression of AtHSP17.6A only improved the osmotic ability of Arabidopsis under drought and salt stress

So far, there have been no reports about the overexpression of class I sHSPs improving plant heat tolerance

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