The invention discloses a sturgeon pathogenic bacterium inhibitor and belongs to the technical field of sturgeon pathogenic breeding. The sturgeon pathogenic bacterium inhibitor comprises the following raw materials in parts by weight: 43-52 parts of sweet wormwood, 10-16 parts of oligopeptide fish dissolution powder, 7-10 parts of choline chloride, 20-28 parts of atractylodes macrocephala, 10-20parts of rhizoma polygonati, 30-35 parts of Baijiu, 0.1-0.3 part of EM (Effective Microorganism) bacteria, 10-15 parts of beer yeast and 10-15 parts of a liver protection agent. A preparation method comprises the following steps: I, preparing the raw materials according to the parts by weight; II, baking the sweet wormwood, fumigating and baking the atractylodes macrocephala and the rhizoma polygonati on the sweet wormwood, crushing into powder, collecting sweet wormwood ash, and coating with choline chloride so as to obtain chloride granules; III, mixing the beer yeast with the EM bacteria, leaving to stand for 3-5 days so as to obtain a bacterial material, and uniformly stirring the oligopeptide fish dissolution powder, the atractylodes macrocephala powder, the rhizoma polygonati powderand the liver protection agent so as to obtain a mixture; IV, coating the chloride granules with a bacterial material, and further coating the outermost layer with the mixture, thereby obtaining the sturgeon pathogenic bacterium inhibitor. The sturgeon pathogenic bacterium inhibitor disclosed by the scheme of the invention is capable of remarkably increasing the ratio of beneficial bacterium florae in intestinal tracts of sturgeon pathogenic.