Method for co-transforming sps, hmgr and dxs genes to cultivate Artemisia annua with high artemisinin content in flower buds

A technology of co-transformation and artichoke, applied in the field of genetic engineering to cultivate plants, can solve the problems of limited drug effect and low toxicity
CN104531753BInactive Publication Date: 2017-08-08CHONGQING MEDICAL & PHARMA COLLEGE
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHONGQING MEDICAL & PHARMA COLLEGE
Publication Date
2017-08-08
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for cultivating sweet wormwood herb with high artemisinin content in flower buds by co-transforming Sps, Hmgr and Dxs genes, relating to the technical field of biology. The method comprises the following steps: cloning three genes namely Anti-Sps, Anti-Hmgr and Anti-Dxs genes from sweet wormwood herb, constructing a plant expression vector containing three genes namely the Sps, Hmgr and Dxs genes which are combined in pairs, performing mediation by using agrobacterium, transferring the three genes namely the Sps, Hmgr and Dxs genes into sweet wormwood herb, and cultivating and screening regenerated plants to obtain a transgenic sweet wormwood herb plant with increased artemisinin content in the flower buds. When the content of non-transformed comparison sweet wormwood herb is 6.20mg / g of dry weight, the maximum artemisinin content in the flower buds of an obtained gene engineering sweet wormwood herb plant disclosed by the invention is 12.10mg / g of dry weight, and the artemisinin content in the flower buds is 1.95 times of the content of non-transformed comparison sweet wormwood herb. The method disclosed by the invention has great significance in saving costs for pharmaceutical factories which take sweet wormwood herb as a raw material.
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Description

technical field

[0001] The invention relates to the field of cultivating plants by genetic engineering, in particular to a method for cultivating Artemisia annua with high artemisinin content by using transgenic technology. Background technique

[0002] Artemisiae annie L., Asteraceae, Artemisia. The scientific name is Artemisia annua, also known as Artemisia stinky, Artemisia annua, and Artemisia annua. It grows on hillsides, forest margins and wastelands, and is an annual herb. Ordinary people use it to relieve summer heat, headache, fever and cold. The extraction of artemisinin is to use the aerial parts of Artemisia annua leaves and unopened flower buds to extract and produce effective physiologically active components such as artemisinin and other products. It is a new antimalarial drug with low toxicity, high efficiency and quick effect (Chinese Pharmacopoeia, 2005; Zhong Guoyue , et al., 2007). Factors that affect the artemisinin content of Artemisia annua include...

Claims

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