Artemisia annua amorpha-4,11-diene synthase mutant with improved enzyme activity and application thereof

A kind of amorphadiene and mutant technology, which is applied in the direction of application, lyase, and introduction of foreign genetic material by using vectors, which can solve the problems of high cost and complicated artemisinin process
CN102732499BInactive Publication Date: 2014-07-16CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
Publication Date
2014-07-16
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to an Artemisia annua amorpha-4,11-diene synthase mutant with improved enzyme activity and application thereof. The amorpha-4,11-diene synthase mutant has enzyme activity substantially higher than that of wild type amorpha-4,11-diene synthase under the condition that catalysate maintains unchanged. Compared to a traditional method, a method of applying the Artemisia annua amorpha-4,11-diene synthase mutant provided by the invention in enzymatic production of artemisinin has the advantages of high efficiency and low cost.
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Description

technical field

[0001] The invention belongs to the field of biotechnology; more specifically, the invention relates to a mutant of Artemisia amorphadiene synthase with improved enzyme activity and application thereof. Background technique

[0002] Artemisinin is a peroxybridge-containing sesquiterpene lactone isolated and identified for the first time by Chinese scientists from Artemisia annua. It is currently the most effective drug for the treatment of cerebral malaria and chloroquine-resistant falciparum malaria. Artemisinin-based combined therapy has been confirmed and recommended by the World Health Organization and Western countries. The content of artemisinin in Artemisia annua is low (0.01-1% dry weight), and it is greatly affected by species and regional planting, and resources are in short supply.

[0003] In the biosynthesis of artemisinin, the sesquiterpene component amorphadiene (Amorphadiene, Amorpha-4, 11-diene) is synthesized by the amorphadiene synthase (a...

Claims

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