Production method of high-artemisinin-content transgene sweet wormwood plants

A production method and artemisinin technology, applied in the fields of botanical equipment and methods, plant products, measuring devices, etc., can solve the problems of difficulty in artificial synthesis, restrictions on large-scale commercial production, and no detection of artemisinin, etc., To achieve the effect of stabilizing the new drug source
CN102776212AInactive Publication Date: 2012-11-14SHANGHAI JIAO TONG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI JIAO TONG UNIV
Publication Date
2012-11-14
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a production method of high-artemisinin-content transgene sweet wormwood plants in the biological technical field. The production method comprises the following steps that three genes including ADS, CYP71AV1 and CPR are cloned from sweet wormwoods, plant expression carriers containing the ADS, CYP71AV1 and CPR genes are built, agrobacterium tumefaciens are used for mediating, the ADS, CYP71AV1 and CPR genes are transferred into the sweet wormwoods, in addition, the plants are regenerated, the screening is carried out, and the transgene sweet wormwood plants with the improved artemisinin content are obtained. When the uninverted ordinary artemisinin content is 6.43mg / g DW, the artemisinin content in the obtained transgene sweet wormwood plant system is as high as 15.09 mg / g DW, and is 2.35 times of the content in the uninverted ordinary artemisinin. The method has the important significance on providing high-yield and stable new medicine sources for the large-scale production of the artemisinin.
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Description

technical field

[0001] The invention relates to a method for increasing the content of artemisinin in the field of biotechnology, in particular to a method for producing transgenic Artemisia annua plants with high artemisinin content. Background technique

[0002] Artemisia annua L. is an annual herb belonging to the family Asteraceae. Artemisinin (artemisinin) is a sesquiterpene lactone compound containing a peroxide bridge structure isolated from its aerial part. It is currently the most effective drug for treating malaria in the world, especially for cerebral malaria and anti-malaria Chloroquine malaria has the characteristics of quick action and low toxicity. Currently, the most effective treatment for malaria recommended by the World Health Organization is artemisinin combination therapy (ACTs). In addition, with the gradual deepening of pharmacological research on artemisinin, scientists have found that artemisinin and its derivatives also have anti-inflammatory, ant...

Claims

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