Artemisia apiacea bHLH transcription factor as well as encoding gene and application thereof
A coding gene and coding technology, which is applied in application, genetic engineering, plant genetic improvement, etc., can solve problems such as unsatisfactory, achieve the effect of overcoming negative feedback, facilitating large-scale industrial production, and simple production process
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[0041] Example 1. Acquisition and functional verification of proteins related to artemisinin synthesis and their coding genes
[0042] 1. Obtain the protein related to artemisinin synthesis and its coding gene AabHLH1
[0043] The total RNA of the leaves of Artemisia annua (Artemisia annua) (the seeds of Artemisia annua were collected from Youyang, Chongqing, China) was extracted using the plant RNA extraction kit of Tiangen Biotechnology Company, and the total RNA was extracted by the reverse transcription kit of Tiangen Biotechnology Company. Total RNA was reverse transcribed into cDNA. The AabHLH1 transcription factor gene was amplified by PCR using the cDNA as a template. The primers used for PCR amplification are PE1 and PE2, and the nucleotide sequences of PE1 and PE2 are as follows:
[0044] PE1: 5'-GAT AAGCTT GCATGACGGAGTACCGCATGAATC-3' (the underlined part indicates the HindIII recognition site);
[0045] PE2: 5'-GTA GCGGCCGC CTACAGCTTCGCTAATTGCCTTAAC-3' (the u...
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