Artemisia apiacea bHLH transcription factor as well as encoding gene and application thereof

A coding gene and coding technology, which is applied in application, genetic engineering, plant genetic improvement, etc., can solve problems such as unsatisfactory, achieve the effect of overcoming negative feedback, facilitating large-scale industrial production, and simple production process

Inactive Publication Date: 2012-03-14
GRADUATE SCHOOL OF THE CHINESE ACAD OF SCI GSCAS
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main source of artemisinin is still extracted from the plant Artemisia annua through traditional techniques, but the cont

Method used

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  • Artemisia apiacea bHLH transcription factor as well as encoding gene and application thereof
  • Artemisia apiacea bHLH transcription factor as well as encoding gene and application thereof
  • Artemisia apiacea bHLH transcription factor as well as encoding gene and application thereof

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Embodiment 1

[0041] Example 1. Acquisition and functional verification of proteins related to artemisinin synthesis and their coding genes

[0042] 1. Obtain the protein related to artemisinin synthesis and its coding gene AabHLH1

[0043] The total RNA of the leaves of Artemisia annua (Artemisia annua) (the seeds of Artemisia annua were collected from Youyang, Chongqing, China) was extracted using the plant RNA extraction kit of Tiangen Biotechnology Company, and the total RNA was extracted by the reverse transcription kit of Tiangen Biotechnology Company. Total RNA was reverse transcribed into cDNA. The AabHLH1 transcription factor gene was amplified by PCR using the cDNA as a template. The primers used for PCR amplification are PE1 and PE2, and the nucleotide sequences of PE1 and PE2 are as follows:

[0044] PE1: 5'-GAT AAGCTT GCATGACGGAGTACCGCATGAATC-3' (the underlined part indicates the HindIII recognition site);

[0045] PE2: 5'-GTA GCGGCCGC CTACAGCTTCGCTAATTGCCTTAAC-3' (the u...

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Abstract

The invention discloses an artemisia apiacea bHLH transcription factor as well as an encoding gene and an application thereof. The artemisia apiacea bHLH transcription factor provided by the invention is protein shown as a) or b): a) protein which consists of amino acid sequences and is shown in sequences 2 in a sequence table; and b) protein which is derived from a), is formed through substituting and/or deleting and/or adding amino acid sequences shown by the sequences 2 in the sequence table through one or a plurality of amino acid residues and is relevant to the artemisinin synthesis. The bHLH transcription factor is instantaneously translated into artemisia apiacea plants to be overexpressed, the expression quantity of the key enzyme in regulated and controlled artemisinin biosynthetic metabolism is greatly improved, and the artemisia apiacea bHLH transcription factor can be used for producing artemisinin. The regulated and controlled artemisinin synthesis method solves the problem of raw material limitation, has a simple production process and is favorable for the large-scale industrial production of the artemisinin.

Description

technical field [0001] The invention relates to the artemisia annua bHLH transcription factor and its coding gene and application in the field of biotechnology. Background technique [0002] The bHLH family of transcription factors is a unique type of transcriptional regulatory factor in eukaryotes, which has a basic / helix-loop-helix element, which can bind to a specific cis-element E-box (CANNTG) and participate in transcription. Studies have shown that bHLH transcription factors are ubiquitous in eukaryotes. In animals, bHLH family proteins play an extremely important role in the regulation of growth and development of organisms, and they participate in the regulation of neuron generation, myocyte generation, hematopoiesis, sex determination and intestinal tissue development. [0003] In plants, the research on bHLH transcription factors mainly focuses on two aspects of biochemistry and secondary metabolism. For example, it is involved in the differentiation of Arabidops...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21C12N15/11C12P17/18A01H5/00
Inventor 王红冀云鹏马东明郝鹤李星
Owner GRADUATE SCHOOL OF THE CHINESE ACAD OF SCI GSCAS
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