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AaWBC1 gene promoter as well as functional verification method and application thereof

A technology for functional verification and promoter, applied in application, genetic engineering, botanical equipment and methods, etc., can solve the problem that the promoter sequence of AaWBC1 gene has not yet been found.

Active Publication Date: 2018-08-24
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After searching the prior art literature, it is found that there is no report related to the AaWBC1 gene promoter sequence of the present invention

Method used

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  • AaWBC1 gene promoter as well as functional verification method and application thereof
  • AaWBC1 gene promoter as well as functional verification method and application thereof
  • AaWBC1 gene promoter as well as functional verification method and application thereof

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Experimental program
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Embodiment

[0039] This embodiment relates to the acquisition of the AaWBC1 gene promoter, which specifically includes the following steps:

[0040] Step 1. Cultivate aseptic seedlings of Artemisia annua

[0041] Artemisia annua seeds were soaked in ethanol with a volume fraction of 75% for 1 min, then soaked in 20% (w / v) NaClO for 20 min, rinsed with sterile water for 3 to 4 times, blotted the surface moisture with sterile absorbent paper, and inoculated Cultivate on a hormone-free MS solid medium at 25°C under 16h / 8h (light / dark) light, and after 14 days, you can obtain an Artemisia annua sterile vaccine;

[0042] Step 2, according to the AaWBC1 gene promoter sequence in the whole genome of Artemisia annua, clone the promoter sequence;

[0043] 1. Genomic DNA extraction

[0044] Put a leaf of Artemisia annua (1cm 2About the size, take it in an ice box), add 2 steel balls. Add 300 μL TPS buffer (operate in a fume hood, TPS contains mercaptoethanol), shake at 55-60 Hz for 90 seconds. ...

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Abstract

The invention discloses an AaWBC1 gene promoter; the promoter can regulate and control the specific expression of an AaWBC1 gene in the secretory glandular hairs of sweet wormwood, and the nucleotidesequence of the promoter is shown in SEQ ID NO. 1. Furthermore, the invention discloses the AaWBC1 gene promoter as well as a functional verification method and application thereof. The promoter provided by the invention can guide the specific expression of the reporter gene in the secretory glandular hairs of the plant, thus having a great significance for the genetic engineering breeding which uses plant glandular hair tissues to express and produce metabolites.

Description

technical field [0001] The invention relates to a promoter (proWBC1) and its application, in particular to a transporter gene promoter specifically expressed in Artemisia annua secretory glandular hairs and its use in the technical field of genetic engineering. Background technique [0002] Artemisia annua L. is an annual herb of the genus Artemisia in the family Asteraceae. The plant has a strong volatile aroma. It is a traditional Chinese herbal medicine in my country. The sesquiterpene lactone compound has the characteristics of quick action and low toxicity for cerebral malaria and anti-chloroquine malaria. Currently, artemisinin combination therapies (ACTs) are the most effective treatment for malaria recommended by the World Health Organization. In addition, artemisinin and its derivatives also have the functions of anti-lupus erythematosus, anti-inflammation, anti-tumor and immune regulation, and are a kind of natural broad-spectrum drug with great potential. There a...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12N15/82C12Q1/6895A01H5/00A01H6/14
CPCC12N15/8223C12N15/8243C12Q1/6895
Inventor 唐克轩王宇婷付雪晴谢利辉沈乾赵静雅孙小芬
Owner SHANGHAI JIAO TONG UNIV
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