The invention relates to a method for simultaneously measuring contents of 10 chemical components in a four-substance soup decoction by an HPLC-DAD (
high performance liquid chromatography-
diode array detection) method and belongs to the field of traditional Chinese medicinal
component analysis. The method comprises the following steps of (1) preparation of a mixed reference substrate solution; (2) preparation of a test substance solution; (3) measurement method: measuring the concentrations of a mixed reference substance and a test substance by the HPLC-DAD; the chromatographic conditions are that the
chromatographic column is elit C18 (250*4.6mm, and 5 microns), the detection
wavelength is 280nm, the flowing speed is 0.8mL / min, the
column temperature is 30 DEG C, and the sample injection amount is 20 microliters; the
gradient elution conditions are that
isocratic elution is executed by using 2% methyl
alcohol by volume for 0-2.0min, the methyl
alcohol is linearly increased to 20 percent when
isocratic elution is executed for 2.0-15.0min, and the
isocratic elution is executed by using 20% methyl
alcohol for 15.0-25.0min; the methyl alcohol is linearly increased to 100 percent when isocratic
elution is executed for 25.0-42.0min, and the isocratic
elution is executed through 100% methyl alcohol for 42.0-45.0min. The
analysis method for simultaneously measuring the 10 chemical components in the four-substance soup decoction is simple, convenient and high in repetitiveness and is accurate and reliable; a basis is supplied to
quality control on the four-substance soup decoction.