146results about How to "Analytical method is accurate" patented technology

The invention relates to a preparation method of liquid calcium chloride, in particular to a method for producing the liquid calcium chloride by using the waste industrial acid, which belongs to the technology field of chemical industry. The preparation method is characterized in allowing massive limestone and waste hydrochloric acid obtained by acid washing strip steel to react at a certain ratio and neutralizing with lime cream to obtain calcium chloride at a certain concentration. Proved by the production, the preparation method is capable of effectively reducing the production cost and achieving comprehensive utilization of the waste industrial acid and remarkable economic and social benefits.

The invention discloses a hemolytic agent for a blood cell analyzing device. The hemolytic agent can decompose erythrocytes and blood platelets in blood. The invention also discloses a leukocyte classification kit. The leukocyte classification kit comprises the hemolytic agent, a dilute and an inhibitor. The agent provided by the invention is the assorted agent suitable for XFA9500 fully-automatic five-classification blood cell analyzer produced by Nanjing Perlong medical equipment limited company; the agent contains no dye, so that the agent is environment-friendly; by adopting the agent, lymphocyte, monocyte, neutrophile granulocyte, eosinophilic granulocyte and basophilic granulocyte can be directly detected; furthermore, the analysis method is fast and accurate.

The invention discloses a method for rapid detection of the residual mount of sulfanilamide in food. The method comprises the following steps: firstly enabling the sulfanilamide and fluorescamine to perform derivation reaction, further enriching derivative products by using cloud point extraction, adding a sample enrichment and a standard plate prepared from a sulfanilamide standard product on a thin layer silica gel plate, comparing the fluorescence intensities of the two under an ultraviolet lamp to judge the concentration range of the fluorescamine in a sample, and determining the residual amount of the sulfanilamide in the food, wherein a detection limit of the method is 0.08 micron g/mL. The method disclosed by the invention has the advantages of simplicity in operation, small using amount of organic solvent, high detection sensitivity, short detection time, strong specificity and capability of realizing effective separation from interfering substances, is a simple, convenient, fast and accurate analytical method, does not need large-scale instruments, only needs to configure small-scale instruments and equipment, and further has extensive application prospects.

The invention provides a method for testing the alkyl quaternary ammonium salt cationic, which includes the following steps: processing a pretreatment for the test sample; testing with a gas-chromatography-mass spectrometer, setting the injection port temperature of the instrument at 180-350 EDG C, setting the carrier gas flow rate at 0.5-2.0 ml per minute, the chromatographic column is a nonpolar, weak polar or strong polar column, setting the port temperature at 180-300 EDG C; injecting the pretreatmented sample into the chromatographic column through the injection port of the instrument, processing the programmed temperature, from 40-120 EDG C up to 210-320 EDG C, processing the mass spectrometric detection after each component of the decomposition products of the sample is separated by the chromatographic column. The method provided by the invention has the merits of cheap test instrument, safety, accurate and fast analysis method and small disturbance, which causes the alkyl quaternary ammonium salt cationic in the sample to pyrolyze on the injection port, and processes the optimized separation of each component of the pyrolyzed products.

The invention discloses a method for measuring dimethyl fumarate in a product by gas chromatography-mass spectrometry. The method comprises the following steps of: pretreating a dimethyl fumarate product to be detected by using an ultrasonic extraction method; setting the temperature of a sample injection port of a gas chromatography-mass spectrometer to be 240-280 DEG C, a flow rate to be 0.8-1.5 ml/min and the temperature of a port to be 280-300 DEG C, and selecting a weak polar column for a chromatographic column; enabling a sample to enter the chromatographic column through the sample injection port, and carrying out temperature programming on the temperature of the chromatographic column to separate the components of the sample by the chromatographic column; and enabling the components separated by the chromatographic column to enter a mass spectrometric detection part through the port of the gas chromatography-mass spectrometer, wherein an electron impact ion source is adopted as the ion source of the mass spectrometric detection. The method for measuring the dimethyl fumarate in the product by the gas chromatography-mass spectrometry has low detection limit, good detection effect, little consumption of analysis solvent and low detection cost.

The invention provides a method for detecting polycyclic aromatic hydrocarbon in food contact material by gas chromatogram-mass spectrometry coupling technique, comprising: Soxhlet extraction method or ultrasonic extraction method is used for extracting the detected food contact material; the extract sample is detected by a gas chromatogram-mass spectrometry coupling instrument; the temperature of a sample inlet of the instrument is set to be 240-320 DEG C, and the flow rate of carrier gas is set to be 0.5-2.0ml/min; a weakly polar chromatographic column is adopted, and the interface temperature is set to be 180-300 DEG C; the pretreated sample enters into the chromatographic column through the sample inlet of the instrument for temperature programming, and the temperature is raised to be 270-330 DEG C from 70-100 DEG C; after being separated by the chromatographic column, all the components in the sample are detected by mass spectrum. The method has the characteristics of low price of the detection instrument, safety, accurate and rapid analysis method and little interference as well as being suitable for standard analysis and detection which can lead all the components of the polycyclic aromatic hydrocarbon in the sample to be cracked in a mass spectrum ion source and optimize all the components of the cracked product, etc.

The invention provides a method for detecting the type and content of monosaccharide and oligosaccharide in edible mushroom, which is a method for measuring and analyzing by an ion chromatographic electrochemical detection means. The ion chromatogram obtained by the method can be used for determining the type and content of the monosaccharide in the edible mushroom according to peak migration time and peak area. The method realizes the accurate control over the monosaccharide and oligosaccharide components in edible mushroom.

The invention discloses a food-borne pathogenic bacteria detection immuno-sensor and a preparation method thereof. The method comprises the following steps of: performing array spreading on DNA, attaching gold sol particles to the skeleton of the DNA, modifying specific antibodies on a DNA conductive chain, immobilizing the specific immune protein antibodies by using the strong adsorption property of metal granules, and fixing the ordered array structure of the DNA on the electrode layer. The invention provides the accurate and quick analysis method; and the sensor is cheap, portable and easy for popularization and use.

The invention relates to a detection method of halogenated alkyl phosphate and includes the following steps: samples are pre-treated with a Soxhlet extraction method or a supersonic extraction method; a high-performance liquid phase color spectrometer which connects diode array detectors is used for detecting the halogenated alkyl phosphate, and the proportions of the organic flow phases of the spectrometer are set from 50 to 100 percent, and the proportions of the inorganic flow phases are set from 0 to 50 percent, and the flow rates are set from 0.5 to 1.0ml per minute and the injection volume is set from 10 to 40ul and an anti-phase chromatographic column is selected as the chromatographic column; the samples pre-treated are brought into the chromatographic column by the flow phases of the high performance liquid phase color spectrometer, and the temperature of a column temperature box is set from 20 to 40 DEG C and components of the samples are separated from the chromatographic column; the components of the samples separated by the chromatographic column enter the diode array detectors to be detected. The detection method of the invention can prevent the samples from decomposing and is accurate and secure in analysis method.

The invention discloses a biological immune sensor and a detection method thereof, which is a novel sensor technology achieving electrical signal detection of pathogen in foods mainly through an immune chemical reaction principle of specificity between an antibody and an antigen. The detection method of the biological immune sensor is accurate and fast, and a product of the biological immune sensor and the detection method of the biological immune sensor is cheap, portable and easy to popularize.

The invention relates to the technical fields of separation and analysis and particularly discloses a preparation method and application of a magnetic diethylstilbestrol molecularly-imprinted polymer based on an organic-inorganic hybridizing technology. The preparation method comprises the steps of preparing ferroferric oxide particles by using a coprecipitation method; preparing silanized ferroferric oxide by using a stober method; preparing an organic and inorganic hybrid magnetic diethylstilbestrol molecularly-imprinted polymer by using a bulk polymerization method; and grinding the polymer, sieving, adding an eluent to extract and elute, and then, drying to obtain the magnetic diethylstilbestrol molecularly-imprinted polymer. A target product, namely diethylstilbestrol, can be rapidly combined on the surface of the magnetic diethylstilbestrol molecularly-imprinted polymer synthesized by using the preparation method and can be rapidly separated from a reaction system under the action of an external magnetic field, so that the aim of efficiently separating a trace amount of diethylstilbestrol from a complex matrix at high specificity is achieved, and the sample pretreatment efficiency is greatly increased.

The invention relates to a method for automatic and comprehensive online analysis of genome sequences. The method comprises the following steps of 1, uploading sample data; 2, analyzing the sample data; 3, querying an analysis result of a single sample; 4, conducting combination analysis of multiple samples; 5, querying a combination analysis result. According to the method for the automatic and comprehensive online analysis of the genome sequences, an innovative mode of gene sequence data management is put forward, and not only can gene sequence data of multiple human individuals be stored simultaneously, but also it can be achieved that the gene sequence data corresponds to basic information of the individuals; a more accurate, faster and more comprehensive analysis method of the genome sequences is put forward, and the more complete annotation of genetic information is achieved; a novel, personalized and visualized online display mode of analysis results is put forward, and the method is suitable for ordinary people and doctors or research personals related to biomedicine and can well meet the needs of practical application.

The invention relates to a method for simultaneously measuring contents of 10 chemical components in a four-substance soup decoction by an HPLC-DAD (high performance liquid chromatography-diode array detection) method and belongs to the field of traditional Chinese medicinal component analysis. The method comprises the following steps of (1) preparation of a mixed reference substrate solution; (2) preparation of a test substance solution; (3) measurement method: measuring the concentrations of a mixed reference substance and a test substance by the HPLC-DAD; the chromatographic conditions are that the chromatographic column is elit C18 (250*4.6mm, and 5 microns), the detection wavelength is 280nm, the flowing speed is 0.8mL/min, the column temperature is 30 DEG C, and the sample injection amount is 20 microliters; the gradient elution conditions are that isocratic elution is executed by using 2% methyl alcohol by volume for 0-2.0min, the methyl alcohol is linearly increased to 20 percent when isocratic elution is executed for 2.0-15.0min, and the isocratic elution is executed by using 20% methyl alcohol for 15.0-25.0min; the methyl alcohol is linearly increased to 100 percent when isocratic elution is executed for 25.0-42.0min, and the isocratic elution is executed through 100% methyl alcohol for 42.0-45.0min. The analysis method for simultaneously measuring the 10 chemical components in the four-substance soup decoction is simple, convenient and high in repetitiveness and is accurate and reliable; a basis is supplied to quality control on the four-substance soup decoction.

The invention relates to a method for detecting tripolycyanamide in an aquatic product via pressurized capillary electrochromatography, which comprises the steps of: 1) sample preparation; 2) sample pretreatment: centrifugal extraction of a sample by mixed solution of trichloroacetic acid with the mass concentration of 0.8-1.2 percent and acetonitrile at the volume ratio of (1.5-2.5):1, purification by an MCX (methoxycinnamate) solid-phase extraction column, and filtration by a filter film of 0.20-0.24mum for determination via the pressurized capillary electrochromatography; 3) preparation of standard tripolycyanamide solution; 4) analysis via the pressurized capillary electrochromatography; and 5) detection and analysis of the sample, quantitative analysis according to the retention time, and then quantitative analysis in an external marking method according to the peak area. The analytical method is efficient, accurate and highly sensitive, and the demands of drug detection of the tripolycyanamide in the daily aquatic product can be satisfied.

The invention provides a preparation method of an adsorbent material. The preparation method comprises the following steps: A), activated carbon fiber is impregnated in hydrogen peroxide and then washed; B), the activated carbon fiber obtained in the step A) is impregnated in an alkaline solution and washed, and the alkaline solution adopts a potassium hydroxide solution or a sodium hydroxide solution; and C), the activated carbon fiber obtained in the step B) is aged under protection of nitrogen to obtain the adsorbent material. According to the preparation method, hydrogen peroxide and the alkaline solution are adopted sequentially to modify the activated carbon fiber, so that basic groups in the activated carbon fiber are increased, and the adsorbability of the activated carbon fiber for formaldehyde, NO2, SO2, benzene and toluene is reinforced. The invention further provides an analytical method for pollutants in air by using the adsorbent material. With adoption of the analytical method, formaldehyde, NO2, SO2, benzene and toluene in the air can be analyzed simultaneously, and the accuracy is higher.

The invention discloses a method for quantitatively determining phenolic acid compounds in barley grains. The method comprises the following steps of: aiming at the physicochemical properties of phenolic acid, extracting the phenolic acid compounds from the barley grains by using a methanol solution at a certain concentration; performing centrifugation on extract; concentrating and filtering the extract; loading the prepared sample extract; and eluting and separating the prepared sample extract by using a methanol-methane acid aqueous solution. The analytical method is accurate, simple and convenient, has high sensitivity, is high in selectivity and repetitiveness; by the method, the types of the phenolic acid compounds in the barley grains can be comprehensively tested at one time; and compared with the conventional method, the method has the advantage that: analysis time is greatly shortened, so that basic requirements for quickly determining and evaluating the phenolic acid compounds in the barley grains on a large scale during production can be met; and the method is suitable for grains of any barley variety.

The invention relates to a method for identifying bee pollen based on kaempferol 3-O-beta-D-glucose-(2-1)-beta-D-glucoside. The method comprises the following steps: preparing a standard stock solution and a standard working solution, pretreating bee pollen, and detecting by using a liquid chromatogram-tandem mass spectrometry method. The detection result of the method shows that the standard curve relevance coefficient is greater than 0.9990, the addition recycling rate ranges from 73.1 percent to 98.4 percent, the relative standard error is less than 6.1 percent, the detection limit is 0.1mu g/g, and the quantitation limit is 0.3mu g/g. The result shows that the established extraction method and an instrument analysis method are accurate and stable and applicable to qualitative and quantitative analysis on kaempferol 3-O-beta-D-glucose-(2-1)-beta-D-glucoside.

The invention provides a method for acquiring the logged-on user password from memory mirroring documents of a 64-bit Windows operation system. The method comprises the following steps: (a) acquiring version information of a system; (b) acquiring the value of a PEB structure variable from aCR3 register and a process environment block of lsass.exe process; (c) dumping execution samples of dynamic link library lsasrv.dll and tspkg.dll; (d) acquiring key related data; (e) acquiring user information from lsasrv.dll; (f) acquiring a login user master certificate from the tspkg.dll dumping document; and (g) acquiring the password. The method for acquiring the logged-on user password plaintext is accurate and efficient, has the analysis effect not influenced by password complexity, and is an important means for acquiring user login information from a physical memory mirroring document; and the acquired login user password is an important evidence in computer online evidence collection.

The invention discloses a precise determination method of extreme working conditions of external heat flow of an orbit of a remote sensor in a whole life cycle, and belongs to the technical field of precise heat control of spatial optical remote sensors. The method specifically includes: 1), selecting parts, which are on a satellite and block the external heat flow of a light entrance of the remote sensor, and parts which are inside the remote sensor and in radiation heat exchange with an optical lens, ignoring radiation, heat capacity and heat conductivity of the back of the optical lens, andestablishing a finite-element heat model of the remote sensor; (2), adopting heat analysis software to calculate temperature of all finite-element nodes of the finite-element heat model of the remotesensor in the whole life cycle according to the preset orbit, and extracting temperature of each finite-element node of the optical lens of the remote sensor in the whole life cycle therefrom; and (3), calculating external heat flow absorbed by each finite-element node of the optical lens of the remote sensor in the whole life cycle according to temperature of the optical lens of the remote sensor in the whole life cycle, and selecting extremum values of the external heat flow to determine the extreme working conditions of the external heat flow of the orbit of the remote sensor in the wholelife cycle. According to the method, calculation is comprehensive, efficiency is high, and precision is high.

The invention relates to the field of analytical chemistry, specifically to an infrared spectroscopic analysis and identification method for the polypide of Cordyceps sinensis. The objective of the invention is to overcome the technical problem that whether the polypide of Cordyceps sinensis is true or false is difficult to determine at present. Thus, the invention provides the method for rapid and accurate discrimination of the polypide of Cordyceps sinensis. The method comprises the following steps: a, drafting the standard spectrum of the polypide of Cordyceps sinensis; b, acquiring necessary infrared spectroscopic characteristic peaks and common infrared spectroscopic characteristic peaks of the polypide of Cordyceps sinensis; and c, comparing infrared spectroscopic characteristic peaks and infrared spectroscopic similarity of a detected sample of the polypide of Cordyceps sinensis. The invention provides the novel method for rapid and accurate discrimination of the polypide of Cordyceps sinensis.