Method for simultaneously measuring contents of 10 chemical components in four-substance soup decoction by HPLC-DAD (high performance liquid chromatography-diode array detection) method

Abstract

The invention relates to a method for simultaneously measuring contents of 10 chemical components in a four-substance soup decoction by an HPLC-DAD (high performance liquid chromatography-diode array detection) method and belongs to the field of traditional Chinese medicinal component analysis. The method comprises the following steps of (1) preparation of a mixed reference substrate solution; (2) preparation of a test substance solution; (3) measurement method: measuring the concentrations of a mixed reference substance and a test substance by the HPLC-DAD; the chromatographic conditions are that the chromatographic column is elit C18 (250*4.6mm, and 5 microns), the detection wavelength is 280nm, the flowing speed is 0.8mL / min, the column temperature is 30 DEG C, and the sample injection amount is 20 microliters; the gradient elution conditions are that isocratic elution is executed by using 2% methyl alcohol by volume for 0-2.0min, the methyl alcohol is linearly increased to 20 percent when isocratic elution is executed for 2.0-15.0min, and the isocratic elution is executed by using 20% methyl alcohol for 15.0-25.0min; the methyl alcohol is linearly increased to 100 percent when isocratic elution is executed for 25.0-42.0min, and the isocratic elution is executed through 100% methyl alcohol for 42.0-45.0min. The analysis method for simultaneously measuring the 10 chemical components in the four-substance soup decoction is simple, convenient and high in repetitiveness and is accurate and reliable; a basis is supplied to quality control on the four-substance soup decoction.

Description

technical field [0001] The invention relates to an HPLC-DAD method for simultaneously measuring the contents of 10 chemical components in Siwu decoction, belonging to the field of component analysis of traditional Chinese medicines. Background technique [0002] Siwu Decoction is contained in "Taiping Huimin Heji Bureau Prescription", which is composed of four herbs: rehmannia glutinosa, angelica, peony root, and chuanxiong. Among them, Rehmannia glutinosa is the monarch drug, which nourishes yin and blood, adds essence and marrow; [1] . Siwu Decoction is a classic prescription for nourishing blood commonly used in clinical practice [2] , has been the basic prescription for nourishing blood and regulating blood since ancient times, and is used to treat various blood deficiency syndromes, such as Chong Ren deficiency, irregular menstruation, metrorrhagia, hard blood lumps, and occasional pain [3-4]. In the existing literature reports, the methods for analyzing the chemical...

AI Technical Summary

Problems solved by technology

In the existing literature reports, the methods for analyzing the chemical components of Siwu Decoction include high performance liquid chromatography and high performance liquid chromatography tandem mass spectrometry [5-61, among which high performance liquid chromatography is used to analyze the chemical components in Siwu Decoction It is difficult to make a more comprehensive evaluation of the quality of Siwu Decoction due to the simultaneous content determination of at most 5 ingredients.

Some scholars have used liquid chromatography-mass spectrometry to simultaneously determine the contents of 9 chemical components in Siwu Decoction, but the cost of liquid mass spectrometry is high, the technology is not common, and the operation steps are more cumbersome than HPLC. Mass spectrometry The peak shape and stability measured by the detector are not as good as those of the UV detector

Images