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Method for identifying proanthocyanidins in ginkgo leaf preparation

A technology of proanthocyanidins and ginkgo biloba, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of endangering the drug safety of the people, fake ginkgo biloba preparations, and low detection standards, so as to achieve easy identification of authenticity and simple operation steps Less, quicker analysis and identification of effects

Active Publication Date: 2012-12-12
ZHEJIANG CONBA PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Due to the low testing standards of national ginkgo leaf preparation products, unscrupulous merchants have mixed other low-priced products into ginkgo biloba preparation products to prepare fake ginkgo leaf preparation products that have passed the test, endangering the drug safety of the people. , extremely dangerous
At present, there is an exclusive method for the detection of ginkgo flavonoids, but its requirements are high and its application is limited
However, there is no relevant report on the identification of true and false proanthocyanidins in ginkgo biloba preparations, so it is a blank in detection

Method used

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  • Method for identifying proanthocyanidins in ginkgo leaf preparation
  • Method for identifying proanthocyanidins in ginkgo leaf preparation
  • Method for identifying proanthocyanidins in ginkgo leaf preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Detection of ginkgo leaf proanthocyanidins and comparison with grape seed proanthocyanidins

[0041] Take homemade Ginkgo biloba proanthocyanidin extract (95% proanthocyanidin content) and grape seed proanthocyanidin extract (96% proanthocyanidin content), respectively dissolve in methanol, filter, and perform HPLC detection:

[0042] Equipment: Aglient 1200 high performance liquid chromatograph; Diamonsil C18 chromatographic column 5μm 4.6×150mm

[0043] Chromatographic conditions:

[0044] (1) The stationary phase is a phenomenex diol column (250×4.6 mm, 5 μm);

[0045] (2) The mobile phase is (A) acetonitrile, (B) methanol / water / acetic acid (95 / 3 / 2, V / V / V); the gradient of the mobile phase is 0~10 min: 0~12 %B; 10~ 10.01 min: 12-15 %B; 10.01-30 min: 15-45 %B; 30-35 min: 45 %B; 35-40 min: 45-0 %B;

[0046] (3) The flow rate is 1.0 ml / min;

[0047] (4) Detection wavelength: fluorescence excitation wavelength 276 nm; emission wavelength 316 nm;

[0048] (...

Embodiment 2

[0050] Example 2 Analysis of proanthocyanidins in Ginkgo biloba leaves

[0051] Sample source: Ginkgo biloba leaves (manufacturer Zhejiang Kangenbei Pharmaceutical Co., Ltd., batch number 20100709)

[0052] Sample 1: Take 2 tablets, grind to fine powder, dissolve with methanol, filter, and obtain.

[0053] Sample 2: Take 2 tablets, grind them to fine powder, dissolve them with 16ml 20% acetone solution and ultrasonic assistance, filter to remove insoluble excipients; apply the filtrate to 30-60 mesh polyamide resin, and use 3 4 times the volume of 20% acetone solution to elute, discard the part; then use 3 to 4 times the volume of 80% acetone solution to elute to obtain the 80% acetone part; recover the 80% acetone part to dryness; the residue is washed with methanol Dissolved to obtain a methanol solution.

[0054] Sample 3: Take 2 tablets, grind them to fine powder, dissolve them with 16ml 20% acetone solution and ultrasonic assistance, filter to remove insoluble excipient...

Embodiment 3

[0057] Example 3 Analysis of Proanthocyanidins in Ginkgo Leaf Capsules

[0058] Sample source: Tianbaoning Capsules (health care products, produced by Hangzhou Kangenbei Pharmaceutical Co., Ltd., batch number 20110516)

[0059] Take 2 capsules, open the capsules, pour out the contents, grind to a fine powder, dissolve with 16ml 20% acetone solution, ultrasonic assistance, filter, and remove insoluble excipients. Load the filtrate on a 30-60 mesh polyamide resin, elute with 3-4 times the volume of 20% acetone solution after loading, discard the part; then elute with 3-4 times the volume of 80% acetone solution , to obtain 80% acetone part; recover 80% acetone part until there is no acetone smell, extract twice with an equal volume of butyl acetate, discard the organic phase, recover the water phase to dryness; dissolve the residue with methanol to obtain a methanol solution.

[0060] Carry out the separation of proanthocyanidins with the chromatographic condition among the emb...

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Abstract

The invention provides an analytic identification method for identifying that whether samples comprising a ginkgo leaf preparation, foods, healthcare products, medicines and / or cosmetics containing ginkgo leaf extracts and the like contain proanthocyanidins having other sources. The method is characterized in that a sample to be detected and a standard substance are pretreated through purifying with an polyamide resin, extracting with an organic solvent and the like; normal phase-high performance liquid chromatograms of the solution sample of the ginkgo leaf preparation to be detected and the solution substandard substance of a standard ginkgo leaf preparation are respectively established by adopting a phenomenex dihydric alcohol as a stationary phase, adopting a column having parameters of 250*4.6mm and 5mum, adopting (A)acetonitrile-(B)methanol / water / acetic acid (95 / 3 / 2, V / V / V) as a mobile phase, and adopting gradient eluting; and the chromatograms are contrasted and analyzed. The identification method has the advantages of high sensitivity, good resolution and reappearance, and obvious identification characteristic, and can accurately identify if the ginkgo leaf is added with above 0.5% of the proanthocyanidins having the other sources.

Description

technical field [0001] The invention relates to a method for analyzing and identifying an analyte or a sample to be analyzed containing proanthocyanidins, specifically a method for identifying whether proanthocyanidins from other sources are adulterated in ginkgo biloba preparations. Background technique [0002] Ginkgo biloba is one of the oldest tree species surviving in the world. Its preparations and health food have become one of the most popular plant products in the world market today. The medicinal value of Ginkgo biloba extract is very high. It has various functions such as antagonizing platelet activating factor, scavenging free radicals, anti-inflammation, anti-allergy, dilating blood vessels, protecting cardiovascular and cerebrovascular, improving peripheral blood circulation, lowering serum cholesterol and assisting anti-cancer, etc. pharmacological activity. Ginkgolides and flavonoids are the main components that produce the above pharmacological activities, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/14
Inventor 叶剑锋乔洪翔方玲王如伟盛卫国闵会马丽萍姚建标
Owner ZHEJIANG CONBA PHARMA
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