The invention provides a
sindbis virus XJ-160 defective
replicon and a construction method and application thereof. The construction method comprises the steps of constructing an XJ-160
virus particle type replicon carrier pVa-XJ, constructing XJ-160
particle type replicons pVaXJ-EGFP and pVaXJ-GLUC containing reporter genes, constructing a XJ-160 defective
replicon, utilizing Acl I
restriction enzyme to respectively perform single
digestion of the VaXJ-EGFP and the pVaXJ-GLUC and constructing defective particles pVaXJ-EGFP
delta and pVaXJ-GLUC
delta with 1139 base deletions at the positions of non-structural genes. The mechanism of the construction method is that due to the partial absence of non-structural genes regions, a large amount of the reporter genes cannot be expressed normally after cells are introduced into the defective replicon, and non-
structural protein expressed by
virus can remedy and react on expression of the large amount of the reporter genes caused by the defective replicon when
alphavirus infection exists in the cells. The
sindbis virus XJ-160 defective replicon can detect multiple types of
alphavirus, has not reaction on non-
alphavirus and is high in specificity and sensitivity and can detect 1PFU
virus. Operation is simple and quick, required instruments are few,
sustainability is strong, the virus is not required to be killed in detection, and the
sindbis virus XJ-160 defective replicon is suitable for quick identification of the alphavirus.