Method for measuring vitamin C by gold nano particulate spectrophotometry

Abstract

The invention discloses a method for determining vitamin C by a golden nanometer particle spectrophotometry way. In a 5mL colorimetric cylinder, sequentially 0.1-2.0 mL HAuCl4 in concentration 95.64 Mu g / mL, 0.02-0.50 mL Sodium Citrate in concentration 1%, and 0.001-2.0mL vitamin C solvent in concentration 0.38mg / mL are added, mixed evenly, then some redistilled water is added to the graduation, mixed fully again; the absorption value is measured on a spectrophometer; meanwhile a blank test is conducted. The determining method is simple and rapid, the instrument used is cheap, and the reagent is easily available.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a method for measuring vitamin C by gold nanoparticle spectrophotometry. Background technique [0002] At present, methods for determining vitamin C include derivative spectrometry, classic iodometric method, spectrophotometry, catalytic kinetic photometry, chromatography, 2,4-dinitrophenylhydrazine colorimetry, flow injection (FIA) Photometric method, electrochemical method and so on. Most of these methods have a number of significant disadvantages. For example, the calibration substance arsenic trioxide used in the iodometric method is a highly toxic substance, and the operation process is relatively cumbersome; the operation of the 2,4-dinitrophenylhydrazine colorimetric method is complicated, the specificity is poor, and it is easily affected by coexisting substances. The measured values ​​are often high. Moreover, the oxidation of activated carbon to ascorbic acid is b...

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Problems solved by technology

For example, the calibration substance arsenic trioxide used in the iodometric method is a highly toxic substance, and the operation process is relatively cumbersome; the operation of the 2,4-dinitrophenylhydrazine colorimetric method is complicated, the specificity is poor, and it is easily affected by coexisting substances. Measured values ​​are often high

Moreover, the oxidation of activated carbon to ascorbic acid is based on the interface reaction of oxygen adsorbed on its surface. If the amount added is too low, the oxidation will not be complete, and the measurement result will be low. The working state of the injection (FIA) photometric test instrument and the control of test conditions are important factors affecting the test results. The concentration of the test sample needs to be controlled within a certain range to obtain good results. The completeness and stability of the determination of the reaction are important for the test The results also have some influence

There is no report on the determination of vitamin C by gold nanoparticle spectrophotometry