A method for removing sterols in herbivorous insect artificial feed

A kind of artificial feed and herbivorous technology, which is applied in the forming or processing of animal feed, animal feed, animal feed, etc., can solve the problems of nutritional composition change, etc., and achieve the effect of short removal cycle, wide application range and less equipment

Active Publication Date: 2019-11-08
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because these methods of removing sterols are only to extract sterols and a part of non-sterol nutrients together with extractants extensively, without further separation, which will cause changes in other nutrients in the raw materials

Method used

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  • A method for removing sterols in herbivorous insect artificial feed
  • A method for removing sterols in herbivorous insect artificial feed
  • A method for removing sterols in herbivorous insect artificial feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Taking corn seed meal as an example, a method for removing sterols in the artificial diet of herbivorous insects includes the following steps:

[0038] In the first step, weigh 100g of corn seed powder and place it in a 900mL beaker, add 300mL of dehydrated ethanol with a purity of analytical grade, then put a magnetic bar with a size of 4cm×0.8cm, and place the beaker on a magnetic stirrer 600r / min, stirred for 2h and then stood still for 3h, sucked out the supernatant and placed it in the reagent bottle; then added 300mL of dehydrated ethanol with a purity of analytical grade to the beaker, stirred the mixed solution, stood still, Extract the supernatant, repeat the above steps for a total of four times; combine the absolute ethanol supernatants taken out five times;

[0039] In the second step, add 300mL of analytically pure n-hexane to the lower precipitate of the first step, and repeat the extraction five times by using the method of extracting the supernatant in t...

Embodiment 2

[0053] Taking wheat flour as raw material to carry out desterolization test according to the method of embodiment 1.

Embodiment 3

[0055] Using soybean flour as raw material, the desterolization test was carried out according to the method in Example 1.

[0056] In three tests, the removal rate of sterol is as table 1:

[0057] Table 1 Sterol removal rate

[0058]

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PUM

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Abstract

The invention provides a method of removing sterols in artificial feed for phytophagous insects. The method comprises the steps of: 1) adding anhydrous ethanol to a container filled with plant seed powder, stirring the mixture and allowing the mixture to stand, and sucking a supernatant away; 2) adding n-hexane, stirring the mixture and allowing the mixture to stand, and sucking a supernatant away; 3) evaporative-drying the supernatants in the steps 1) and 2), and performing elution separation in a column chromatography manner; 4) separating the liquid, after elution in the step 3) into two samples, one with the sterols, the other without the sterols; 5) through thin-layer chromatography, separating the sample with the sterols in the step 4), and respectively scraping zones with and without the sterols; 6) extracting the sterols in the step 5), and performing elution separation in a column chromatography manner; and 7) mixing the substances without the sterols in the steps 1) to 6), and removing the solvent to prepare a sterol-free feed raw material. The method is low in technical requirement, is easy to carry out, is short in removal period and is free of loss of other nutrients except the sterols.

Description

technical field [0001] The invention relates to the field of insect artificial feed, in particular to a method for removing sterols from herbivorous insect artificial feed. Background technique [0002] Sterols are the general term for four-membered ring lipids, most of which are similar in structure. The basic skeleton is cyclopentane polyhydrophenanthrene nucleus, which consists of three six-membered rings and one five-membered ring. The difference between different sterols lies in the C-3 position The functional groups connected, the side chains connected to the C-17 position, and the position of the double bond on the side chain are as follows: [0003] [0004] Sterols include animal sterols (cholesterol) and phytosterols. At present, more than 250 sterols have been identified, and they are mainly in the free state, sterol esters (fatty acid esters and phenolic esters), steryl glycosides and acylated steryl glycosides, etc. form exists. Common free sterols include ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K50/90A23K40/00A23K10/30A23L5/20
Inventor 郭义张礼生刘晨曦王孟卿陈红印
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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