Elisa kit for detecting carbofuran in aquatic product

Abstract

The invention relates to an enzyme linked immunosorbent assay kit for detecting the residual amount of carbofuran in an aquatic product. The kit comprises a 96-hole elisa plate, an elisa secondary antibody, a carbofuran antibody working solution, carbofuran standard solutions with six concentrations, substrate color developing solutions, a stopping solution, a concentrated washing solution, a concentrated composite solution, a high-concentration standard, a cover plate membrane, a self-sealing bag, a description and a quality report. An indirect competitive ELISA method is adopted; holes formed in the elisa plate are pre-coated with conjugation antigens, carbofuran contained in a sample and the conjugation antigens pre-applied to the micro holes compete for combining carbofuran antibodies, and after the elisa secondary antibody is added, substrate color developing is performed with TMB; a sample absorbance value is compared with a standard curve and then multiplied by the corresponding dilution ratio, and then the residual amount of carbofuran in the sample can be obtained. Compared with an instrument assay technique, the kit has the advantages of being convenient to use, high in sensitivity and the like and can play an important role in residual amount detection of carfuran in the aquatic product.

Description

technical field [0001] The invention relates to a kit for detecting carbofuran residues, in particular to an enzyme-linked immunoassay (ELISA) kit for detecting carbofuran residues in aquatic products. An enzyme immunoassay developed after fluorescence and radioimmunoassays. Background technique [0002] Carbofuran is a carbamate insecticide and nematicide. Created by the United States in 1963 and promoted in 1967. The pure product is white crystal, the solubility in water is 700ppm at 25°C, it is relatively stable in neutral and acidic conditions, but unstable in alkaline medium, and the hydrolysis speed increases with the increase of PH value and temperature. According to China's pesticide toxicity classification standards, carbofuran is a highly toxic pesticide and cannot be used on vegetables and fruit trees. Carbofuran is the most harmful to birds, as long as a small bird feeds on a grain of carbofuran, it can be fatal. Birds or other insects killed by furan poisoni...

AI Technical Summary

Problems solved by technology

[0003] Another characteristic of carbofuran's environmental behavior is that it has a long residual period in the soil (degradation half-life is 1 to 2 months), and has a large mobility in the soil (water solubility is 700mg / L). 1. Sandy soil areas with shallow groundwater levels are likely to cause groundwater pollution. For this reason, the United States has imposed some regional restrictions on the use of carbofuran