L-methionine product content determination method

A technology for measuring methionine, applied in the direction of polarization influence characteristics, etc., can solve the problems of expensive instrument, poor accuracy, high cost, etc., and achieve the effect of cheap instrument and low cost

Active Publication Date: 2013-12-04
NINGXIA UNISPLENDOUR TIANHUA METHIONINE CO LTD
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  • Application Information

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Problems solved by technology

However, there are certain technical problems in these determination methods: the direct determination by pre-column derivatization high-performance liquid chromatography and amino acid analyzer is accurate, but the equipment used is expensive and the cost is high; the determination accuracy of the formaldehyde method is relatively poor; Using non-aqueous titration method, all alkaline substances (including bases in proton theory) will be measured together, and the sample is required to be anhydrous

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  • L-methionine product content determination method
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  • L-methionine product content determination method

Examples

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Embodiment 1

[0025] A kind of assay method of L-methionine product content, it is specifically carried out according to the following steps:

[0026] ① Weigh 2.1154g of L-methionine reference substance with a known mass percentage of 99.0%, put it into a 100mL measuring bottle, add 5mol / L hydrochloric acid solution to dissolve, dilute to 100mL, shake well, and use it as the reference substance solution. And rinse the measuring tube several times with the reference substance solution, then slowly inject the reference substance solution into the measuring tube, place it in the polarimeter to detect the reading, and obtain the optical rotation of the reference substance solution as 0.995°; wherein, the precision of the polarimeter is 0.001°, the length of the optical rotation tube is 2dm, the measurement condition is the D line (589.3nm) of the sodium spectrum, and the temperature is 23°C;

[0027] ②Take an appropriate amount of the product containing at least 90% of the mass percentage of L-...

Embodiment 2

[0045] A kind of assay method of L-methionine product content, the selection of its reference substance and reference substance solution, mensuration are identical with embodiment 1, and other steps are specifically carried out according to the operating steps described in embodiment 1, and in this example , the quality of the sample to be tested is 2.0075g, the optical rotation of the sample solution to be tested is 0.926°, and the calculated result shows that the content of L-methionine in the sample to be tested is 97.09%.

Embodiment 3

[0047] A kind of assay method of L-methionine product content, the selection of its reference substance and reference substance solution, mensuration are identical with embodiment 1, and other steps are specifically carried out according to the operating steps described in embodiment 1, and in this example , the quality of the sample to be tested is 2.0351g, the optical rotation of the sample solution to be tested is 0.948°, and the calculated result shows that the content of L-methionine in the sample to be tested is 98.05%.

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Abstract

The invention relates to an L-methionine product content determination method. According to the L-methionine product content determination method, an L-methionine product sample is used for preparing a sample solution according to the optical activity of L-methionine and the fact that the optical activity is in direct proportion to a solution concentration within a certain concentration range, the optical activity of the sample solution is tested and contrasted with the optical activity of a reference solution with a corresponding concentration and known mass percentage composition under the same conditions, and then the content of the L-methionine in the product sample is obtained. The L-methionine product content determination method is simple, easy to implement, fast, accurate, low in cost and suitable for content determinination of L-methionine products, and has obvious advantages compared with other methods; moreover, instruments are cheap.

Description

technical field [0001] The invention relates to content determination of L-methionine products, in particular to a method for determining the content of L-methionine products. Background technique [0002] L-methionine, also known as L-2-amino-4-methylthiobutyric acid, is a white solid with a melting point of 281°C, soluble in water, dilute acid and dilute alkali, insoluble in ethanol, ether, etc. L-methionine is one of the amino acids that make up proteins, and is an essential amino acid and ketogenic amino acid for mammals. It has a detoxifying effect, can be used as an amino acid drug and a nutritional supplement, and can maintain the growth and development of the body and nitrogen balance. [0003] At present, there are pre-column derivatization high-performance liquid chromatography, direct determination of amino acid analyzer, formaldehyde method, etc. for the determination of amino acid content. For the determination of L-methionine, the Chinese Pharmacopoeia adopts ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/21
Inventor 罗延谷李青扬胡欣李文艳何咏梅朱丽利
Owner NINGXIA UNISPLENDOUR TIANHUA METHIONINE CO LTD
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