Method for identifying bee pollen based on kaempferol 3-O-beta-D-glucose-(2-1)-beta-D-glucoside

A technology of glucoside and bee pollen, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems that have not been seen in the application of kaempferol in the identification of bee pollen, achieve accurate extraction methods and instrument analysis methods, increase extraction efficiency, and improve methods simple effect

Active Publication Date: 2014-12-03
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0018] At present, there is no application of kaempferol 3-O-β-D

Method used

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  • Method for identifying bee pollen based on kaempferol 3-O-beta-D-glucose-(2-1)-beta-D-glucoside
  • Method for identifying bee pollen based on kaempferol 3-O-beta-D-glucose-(2-1)-beta-D-glucoside
  • Method for identifying bee pollen based on kaempferol 3-O-beta-D-glucose-(2-1)-beta-D-glucoside

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] 1) Preparation of standard stock solution (2.0mg / mL): Accurately weigh 50.0mg of the standard substance kaempferol 3-O-β-D-glucose-(2→1)-β-D-glucoside, and place it in a volume of 25mL In the bottle, dissolve with methanol and dilute to volume, shake well, and prepare a 2.0 mg / mL standard stock solution for future use (storage conditions: sealed, stored at -20°C, stable for 6 months).

[0060] 2) Preparation of standard working solution: Take an appropriate amount of standard stock solution in a 10mL volumetric flask, dilute to volume with methanol successively, and prepare the following series of standard working solutions: 1ng / mL, 10ng / mL, 50ng / mL, 100ng / mL, 500ng / mL and 1000ng / mL, ready for use (preservation conditions: sealed, stored in a refrigerator at 4°C for use, stable for 1 month).

[0061] 3) Bee pollen sample pretreatment method: Weigh 12 parts of evenly crushed bee pollen samples, put about 1.0 per part into the extraction pool, set the temperature at 45°C,...

Embodiment 2

[0072] 1) Preparation of standard stock solution (2.0 mg / mL): same as Example 1.

[0073] 2) Preparation of standard working solution: same as Example 1.

[0074] 3) Pretreatment method of bee pollen samples: Weigh 12 parts of evenly crushed bee pollen samples, put about 1.5g of each into the extraction pool, set the temperature at 50°C, the pressure at 25MPa, and the entrainer concentration at 78%, The flow rate of ethanol was 0.4mL / min, the extraction time was 3.8 hours, and the flow rate of carbon dioxide was 4.0L / h. The extracted product was dilute to 50 mL with ethanol. Draw part of the bee pollen sample solution from the volumetric flask and dilute it 10 times with pure water, and take 1.0 mL for analysis.

[0075] 4) Liquid chromatography-tandem mass spectrometry conditions: the same as in Example 1

[0076] 5) Determination method

[0077] Qualitative determination: with embodiment 1.

[0078] Quantitative determination: with embodiment 1.

[0079] 6) Results: The ...

Embodiment 3

[0083] 1) Preparation of standard stock solution (2.0 mg / mL): same as Example 1.

[0084] 2) Preparation of standard working solution: same as Example 1.

[0085] 3) Pretreatment method of bee pollen samples: Weigh 12 parts of evenly crushed bee pollen samples, put about 2.0 g each into the extraction pool, set the temperature at 50°C, the pressure at 40 MPa, and the entrainer concentration at 80%, The flow rate of ethanol was 0.4mL / min, the extraction time was 4 hours, and the flow rate of carbon dioxide was 2.0L / h. The extracted product was dilute to 50 mL with ethanol. Draw part of the bee pollen sample solution from the volumetric flask and dilute it 10 times with pure water, and take 1.0 mL for analysis.

[0086] 4) Liquid chromatography-tandem mass spectrometry conditions: the same as in Example 1.

[0087] 5) Determination method

[0088] Qualitative determination: with embodiment 1.

[0089] Quantitative determination: with embodiment 1.

[0090] 6) Results: The ...

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Abstract

The invention relates to a method for identifying bee pollen based on kaempferol 3-O-beta-D-glucose-(2-1)-beta-D-glucoside. The method comprises the following steps: preparing a standard stock solution and a standard working solution, pretreating bee pollen, and detecting by using a liquid chromatogram-tandem mass spectrometry method. The detection result of the method shows that the standard curve relevance coefficient is greater than 0.9990, the addition recycling rate ranges from 73.1 percent to 98.4 percent, the relative standard error is less than 6.1 percent, the detection limit is 0.1mu g/g, and the quantitation limit is 0.3mu g/g. The result shows that the established extraction method and an instrument analysis method are accurate and stable and applicable to qualitative and quantitative analysis on kaempferol 3-O-beta-D-glucose-(2-1)-beta-D-glucoside.

Description

technical field [0001] The invention relates to a method for identifying bee pollen, in particular to a method for identifying bee pollen based on the content of kaempferol 3-O-β-D-glucose-(2→1)-β-D-glucoside. Background technique [0002] Bee pollen is the pollen collected by bees from the stamens of nectar plants, plus the bee's own glandular secretions, saliva and nectar to form an irregular oblate mass. [0003] Bee pollen contains a variety of amino acids, vitamins, mineral elements, unsaturated fatty acids, organic acids, proteins, lipids, carbohydrates, polyphenols and flavonoids, polysaccharides, enzymes, nucleic acids, hormones and other active substances. nutritional value. [0004] Bee pollen is rich in various active functions because of its various components. It not only has a special effect on the treatment of anemia, but also has a good effect on the treatment of prostate diseases; it also has the effects of anti-cancer, anti-radiation, lowering blood fat, a...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06
Inventor 周金慧李熠孙丽萍吴黎明徐响
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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