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Biological immune sensor and detection method thereof

A technology of biological immunity and detection method, applied in the field of analysis and detection, can solve the problems of difficult identification, long inspection time, complicated operation, etc., and achieve the effect of easy promotion

Inactive Publication Date: 2012-07-04
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0021] To sum up, with the continuous development of science and technology, more and more food microbial detection technologies have been developed, but the traditional detection methods of foodborne pathogens are not only cumbersome to operate, but also take a long time to detect, generally requiring 5-7 days of culture identification time, the time can not meet the requirements of rapid detection
Furthermore, the identification of bacteria is a time-consuming and complicated task. For example, the detection of Campylobacter jejuni requires 15 kinds of culture media and more than 20 steps of size, and the operation is very complicated.
Moreover, the relative instability of the biochemical characteristics of bacteria also brings great difficulties and uncertainties to the identification of bacteria. At the same time, due to factors such as unstable quality of some biochemical reagents, serum, etc. Inaccurate
At present, the commonly used bacterial identification cards at home and abroad, such as API, although the quality is stable, but there are many human factors in the identification, and the results of different operators are often different. The automatic identification of bacteria has good reproducibility, but it is limited to a few A biochemical reaction, the identification between similar strains is particularly difficult, and the detection efficiency is low

Method used

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  • Biological immune sensor and detection method thereof
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  • Biological immune sensor and detection method thereof

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Embodiment 1

[0052] Embodiment 1: the present invention will be further described below in conjunction with accompanying drawing

[0053] 1. Main solution preparation

[0054] 20×PBS buffer preparation

[0055]

[0056] Add water to dissolve, dilute to 2000ml, and pressurize at 121°C for 20min.

[0057] 1×PBST buffer preparation

[0058]

[0059] Mix well and store in a brown bottle.

[0060] Blocking solution preparation

[0061] Take 0.01g of bovine serum albumin (BSA) and dissolve it in 1ml of 1×PBST buffer, and prepare it for immediate use.

[0062] 2. The concentration of the obtained antigenic protein is 2.26mg / ml, which is distributed into clean and sterile Ep tubes and stored at -80°C. The results of the agar expansion test showed that the titer of the enteritis protein immune serum was 1:32, and the immune serum was also distributed into clean Ep tubes and frozen at -80°C. To be used for subsequent testing.

[0063] 3. The preparation of gold nanoparticles was realize...

Embodiment 2

[0067] Embodiment 2: A detection method of an immunosensor for detecting food-borne Vibrio parahaemolyticus, the operation steps of the method are:

[0068] 1. Synthesis of Au-MWCNT hybrid materials: Figure 4 The morphology photos of the prepared gold-coated carbon nanotubes under different magnifications are given respectively. Au has strong sulfurophilicity, so Au particles and sulfhydryl groups can be easily and strongly linked together. Through TEM and SEM, it can be observed that many spherical Au particles are arranged on the surface of sulfhydryl-modified MWCNT, and the shape and distribution of Au particles can be observed. The Au particles are arranged relatively closely, but the distribution is not very uniform. The average particle size measured is 15nm. TEM and SEM images show that Au particles are distributed along the MWCNTs. Because of the formation of Au-S bonds, Au particles can be arranged on the surface of MWCNTs. The Au-S covalent bond is strong, and t...

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Abstract

The invention discloses a biological immune sensor and a detection method thereof, which is a novel sensor technology achieving electrical signal detection of pathogen in foods mainly through an immune chemical reaction principle of specificity between an antibody and an antigen. The detection method of the biological immune sensor is accurate and fast, and a product of the biological immune sensor and the detection method of the biological immune sensor is cheap, portable and easy to popularize.

Description

technical field [0001] The invention belongs to an analysis and detection technology, and relates to the synthesis and detection application of nanometer materials, in particular to a bioimmune sensor and a detection method for detecting food pathogenic microorganisms. Background technique [0002] Food safety has always been a global issue, and it is the most concerned issue of governments and people in various countries. It is related to the health and safety of consumers. Although the governments of various countries have taken corresponding measures to control and detect food-borne pathogens, and ensure food safety in the form of legislation, due to the globalization of natural food source production, the application of new food production processes, Various factors such as changes in people's eating habits have reduced the safety of food to a certain extent. According to the World Health Organization (WHO), foodborne diseases caused by pathogenic microorganisms are one...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569
Inventor 杨良保刘洪林李民强刘锦淮
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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