Fast instrumental analysis method for aflatoxins in foods

A technology of aflatoxin and aflatoxin, applied in instruments, scientific instruments, analytical materials, etc., can solve the problems of inaccurate characterization and cumbersome processing, and achieve the effects of high recovery rate, simple processing method and accurate analysis method

Inactive Publication Date: 2012-02-01
PONY TESTING INT GRP SHANGHAI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to overcome the shortcomings of traditional detection methods such as cumbersome processing and inaccurate qualitative determination, and provide a method with simple steps that can detect multiple aflatoxins at the same time, which can meet the needs of detecting multiple aflatoxins

Method used

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  • Fast instrumental analysis method for aflatoxins in foods
  • Fast instrumental analysis method for aflatoxins in foods
  • Fast instrumental analysis method for aflatoxins in foods

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] With 0.1% formic acid acetonitrile-water (10:90, V / V) as a solvent, prepare a series of standard solutions with concentrations of aflatoxin mixed solution of 0.1, 0.5, 1.0, 2.0, 5.0 μg / mL respectively, under the above chromatographic conditions The peak area was determined by injection, and quantified by external standard method. Standard solution extracted ion chromatogram as figure 1 , with the peak area y as the ordinate and the concentration x as the abscissa, a linear regression was performed, and the results are shown in Table 1.

[0030] The retention time of table 1 four kinds of aflatoxins, monitoring ion, linear equation and correlation coefficient

[0031]

[0032] Based on the sample mass of 5g and constant volume of 20mL, the detection limits of Aspergillus flavus B1 and G1 in the sample are both 0.2 μg / kg, and the detection limits of Aspergillus flavus B2 and G2 are both 0.04 μg / kg.

Embodiment 2

[0034] Accurately weigh 5g of the crushed flour sample (accurate to 0.01g), extract and measure according to the above-mentioned experimental method, select the flour sample, and carry out three parallel determinations. The experiment numbers are A-1, A-2, and A-3, respectively. The analysis results are shown in Table 2. The first parallel sample (Experiment No. A-1) was measured 5 times in parallel, and the analysis results are shown in Table 3. It can be seen from Table 2 that the relative standard deviations of the four targets of Aspergillus flavus B1, B2, G1 and G2 in the parallel samples are 1.1-4.1%. It can be seen from Table 3 that the relative standard deviation of the repeatability test is between 3.9% and 6.4%.

[0035] Table 2 Test results of four kinds of target objects to be tested in flour samples

[0036]

[0037] Table 3 Flour sample test repeatability

[0038]

Embodiment 3

[0040] The standard solution in Example 1 was added to the flour sample in Example 2, and the experiment was carried out according to the above-mentioned sample pretreatment method and instrumental analysis and detection method. The sample was spiked at two concentrations, and the average value was measured three times for each spiked sample, and the spiked recovery rate of the sample was calculated according to the actual amount added and the measured results. The results are shown in Table 4. It can be seen from Table 4 that the recovery rate of the sample addition is in the range of 83.3-91.6%.

[0041] The recovery rate of standard addition of table 3 flour samples

[0042]

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Abstract

The invention belongs to the technical field of food detection, and particularly relates to a fast instrumental analysis method for aflatoxins in foods. The invention discloses a fast instrumental analysis method for aflatoxins B1, B2, G1 and G2 in foods. The method comprises the following steps of: weighing a certain amount of sample; adding an acetonitrile-water solution in a certain ratio; performing ultrasonic extraction; and selecting specific parent ions and daughter ions by using a super-efficient liquid phase chromatography-mass spectrometer under a gradient elution condition to measure the content of the four types of aflatoxins. The method is simple and is high in efficiency and the precision of measurement results is high. By the method, four monomers of the aflatoxins are completely separated, and the recovery rate is high; and compared with the prior art, the method has the advantages of high speed and accuracy of detection and simple pretreatment, and can be used for accurately determining the nature and the quantity to meet requirements on research and detection.

Description

technical field [0001] The present invention relates to aflatoxin B 1 , B 2 , G 1 , G 2 Rapid instrumental analysis method, especially concerning aflatoxin B in food 1 , B 2 , G 1 , G 2 detection method. Background technique [0002] Aflatoxins (AFT) are a class of toxic mycotoxins produced by the metabolism of Aspergillus flavus and Aspergillus parasiticus fungi. Aflatoxins were found. The current national standard for the detection of aflatoxins is mainly GB / T 5009.23-2006. However, the pretreatment of this method needs to be derivatized, which is cumbersome, and it is easy to cause large losses in the process of processing, and the detection cycle is long, which often cannot meet the rapid and accurate detection requirements of customers. However, there is no rapid instrument for aflatoxin in food yet. Analytical method. Therefore, the establishment of a fast, accurate, convenient and economical detection method is conducive to monitoring the content of aflatox...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/72G01N30/06
Inventor 宋薇
Owner PONY TESTING INT GRP SHANGHAI CO LTD
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