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Leukocyte classification hemolytic agent and kit thereof

A white blood cell classification and hemolytic agent technology, applied in the field of blood cell analysis, can solve the problems of inability to directly analyze basophils, increase equipment complexity and detection cost, and achieve fast and accurate analysis methods and the effect of inhibiting continuous damage

Active Publication Date: 2013-09-25
NANJING PERLONG MEDICAL EQUIP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this system needs to heat the sample at 60-75°C, which increases the complexity of the equipment and the detection cost, and cannot directly analyze basophils

Method used

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  • Leukocyte classification hemolytic agent and kit thereof
  • Leukocyte classification hemolytic agent and kit thereof
  • Leukocyte classification hemolytic agent and kit thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A kind of hemolytic agent, it is characterized in that, described hemolytic agent comprises nonionic surfactant, anionic surfactant, organic acid and preservative, described nonionic surfactant concentration 12.1g / L, anionic surfactant concentration are 2.0g / L, organic acid concentration 1.0ml / L and preservative concentration 0.5g / L.

[0029] The nonionic surfactant is polyoxyethylene laurylphenol ether, the anionic surfactant is sodium dodecylsulfonate, and the mixture of the organic acid formic acid and acetic acid. The preservative is sodium azide. The pH of the hemolytic agent is 2.0. A white blood cell classification kit, said kit comprising:

[0030] a. The hemolytic agent mentioned above;

[0031] b. Diluent, the composition includes: chloride salt, sodium sulfate, disodium hydrogen phosphate, sodium dihydrogen phosphate and preservatives, also including EDTA-Na 2 ;

[0032] c. Inhibitors, comprising: alkali metal salts, salts containing carbonate or bicarbo...

Embodiment 2

[0040] A kind of hemolytic agent, described hemolytic agent comprises nonionic surfactant, anionic surfactant, organic acid and preservative, described nonionic surfactant concentration 6.2g / L, anionic surfactant concentration are 1.2g / L , The organic acid concentration is 1.3ml / L and the preservative concentration is 0.3g / L.

[0041] The nonionic surfactant is saponin, the anionic surfactant is sodium dodecylbenzenesulfonate, and the organic acid is formic acid. The preservative is Proclin300. The pH of the hemolytic agent is 4.0.

[0042] A white blood cell classification kit, said kit comprising:

[0043] a. The hemolytic agent mentioned above;

[0044] b. Diluent, the composition includes: chloride salt, sodium sulfate, disodium hydrogen phosphate, sodium dihydrogen phosphate and preservatives, also including EDTA-Na 2 ;

[0045] c. Inhibitors, comprising: alkali metal salts, salts containing carbonate or bicarbonate ions, and preservatives.

[0046] The preservative i...

Embodiment 3

[0048] On the XFA9500 automatic five-classification blood cell analyzer produced by Nanjing Pulang Medical Equipment Co., Ltd., using the kit of Example 1 of the present invention, the detection results of the same blood measurement 20 times are shown in Table 1:

[0049] Table 1

[0050] Measurement times

WBC

RBC

HGB

HCT

MCV

PLT

LYM%

MON%

NEU%

EOS%

BSA%

1

8.51

4.55

151

47.3

95.6

156

24.5

7.8

63.3

3.0

1.4

2

8.60

4.51

151

47.6

95.5

155

24.7

7.5

63.4

3.0

1.4

3

8.66

4.50

152

47.5

95.7

154

24.9

7.3

63.3

2.9

1.6

4

8.59

4.49

151

47.8

95.4

154

24.2

7.4

64.5

2.4

1.5

5

8.4

4.46

153

47.9

95.1

153

23.9

7.5

65.0

2.9

0.7

6

8.57

4.52

150

47.5

95.6

158

23.6...

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PUM

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Abstract

The invention discloses a hemolytic agent for a blood cell analyzing device. The hemolytic agent can decompose erythrocytes and blood platelets in blood. The invention also discloses a leukocyte classification kit. The leukocyte classification kit comprises the hemolytic agent, a dilute and an inhibitor. The agent provided by the invention is the assorted agent suitable for XFA9500 fully-automatic five-classification blood cell analyzer produced by Nanjing Perlong medical equipment limited company; the agent contains no dye, so that the agent is environment-friendly; by adopting the agent, lymphocyte, monocyte, neutrophile granulocyte, eosinophilic granulocyte and basophilic granulocyte can be directly detected; furthermore, the analysis method is fast and accurate.

Description

technical field [0001] The invention relates to the field of blood cell analysis, in particular to a hemolyzing agent for leukocyte classification and a kit thereof. Background technique [0002] Normal peripheral blood leukocytes are generally divided into five subgroups, namely lymphocytes (LYM), monocytes (MON), neutrophils (NEU), eosinophils (EOS) and basophils ( BASO). In the field of clinical analysis, accurate counting of different subgroups plays a very important role in diagnosis and research, and the changes in the content of each subgroup of white blood cells are used as important indicators for diagnosis and treatment in clinic. For example, viral infections can cause lymphocytosis, while parasitic infections and allergic diseases can cause eosinophilia. [0003] With the continuous development and improvement of the level of science and technology, hospitals currently use automatic blood cell analyzers to analyze white blood cells in peripheral blood. However...

Claims

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Application Information

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IPC IPC(8): G01N33/49
Inventor 尹小东管中来
Owner NANJING PERLONG MEDICAL EQUIP
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