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Method for detecting monosaccharide and oligosaccharide in edible mushroom

A detection method and technology of edible fungus, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of poor selectivity, need for derivatization treatment, low sensitivity, etc., and achieve the effect of expanding sample types

Inactive Publication Date: 2011-01-26
SHANGHAI ACAD OF AGRI SCI
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  • Abstract
  • Description
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Problems solved by technology

However, these methods have disadvantages such as complicated operation, derivatization treatment, low sensitivity and poor selectivity.

Method used

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  • Method for detecting monosaccharide and oligosaccharide in edible mushroom
  • Method for detecting monosaccharide and oligosaccharide in edible mushroom
  • Method for detecting monosaccharide and oligosaccharide in edible mushroom

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Experimental program
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Embodiment Construction

[0012] Five samples of Agaricus bisporus from the producing areas of Shandong, Zhejiang, Wuhan, Shanghai and Fujian were analyzed and tested according to the following operation procedures.

[0013] The samples were dried and pulverized at 70°C. Take 0.1g of dry sample, add 10ml of deionized water, ultrasonically extract at 50°C for one hour, dilute the extract 1000 times, filter the diluted solution through a filter head, and take 1ml of the filtrate to test on the machine. The test results are shown in Table 2.

[0014] Table 2 Detection results of types and contents of monosaccharides and oligosaccharides in dry samples of Agaricus bisporus from different origins

[0015]

[0016] The method has good linearity in the range of 0.1 μg / ml to 20 μg / ml, and the R of the seven sugar standard curves 2 Between 0.9975 and 0.9999, the detection limit is 0.025μg / ml.

[0017] The protection scope of the present invention is not limited to the description made in the embodiment, an...

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Abstract

The invention provides a method for detecting the type and content of monosaccharide and oligosaccharide in edible mushroom, which is a method for measuring and analyzing by an ion chromatographic electrochemical detection means. The ion chromatogram obtained by the method can be used for determining the type and content of the monosaccharide in the edible mushroom according to peak migration time and peak area. The method realizes the accurate control over the monosaccharide and oligosaccharide components in edible mushroom.

Description

Technical field [0001] The invention relates to a technology for separation and detection of monosaccharides and oligosaccharides in edible fungi, in particular to the separation and detection of seven kinds of monosaccharides and oligosaccharides in edible fungi by ion chromatography. Background technique [0002] In recent years, with the increase of people's demand for healthy food, edible fungus, as a kind of agricultural product rich in various nutrients and unique flavor, has gradually attracted people's attention, and its production and consumption have gradually increased. As an important nutrient in food, sugar has physiological functions such as supplying heat, maintaining blood sugar constant, forming tissues, protecting liver and detoxifying, saving protein consumption in the body, and maintaining fat metabolism. The content of sugar also marks the nutritional value of the product; Important flavor substances in food, the type and content of natural small molecul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 刘海燕邢增涛邵毅张娟琴赵晓燕
Owner SHANGHAI ACAD OF AGRI SCI
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