Food-borne pathogenic bacteria detection immuno-sensor and preparation method thereof

Abstract

The invention discloses a food-borne pathogenic bacteria detection immuno-sensor and a preparation method thereof. The method comprises the following steps of: performing array spreading on DNA, attaching gold sol particles to the skeleton of the DNA, modifying specific antibodies on a DNA conductive chain, immobilizing the specific immune protein antibodies by using the strong adsorption property of metal granules, and fixing the ordered array structure of the DNA on the electrode layer. The invention provides the accurate and quick analysis method; and the sensor is cheap, portable and easy for popularization and use.

Description

technical field [0001] The invention relates to the technical field of new materials, including the synthesis of nanomaterials and the construction of devices, and in particular to a sensor for the rapid detection of pathogenic comb organisms in food safety and a preparation method thereof. Background technique [0002] Food safety has always been a global issue, and it is the most concerned issue of governments and people in various countries. It is related to the health and safety of consumers. Although the governments of various countries have taken corresponding measures to control and detect food-borne pathogens, and ensure food safety in the form of legislation, due to the globalization of natural food source production, the application of new food production processes, Various factors such as changes in people's eating habits have reduced the safety of food to a certain extent. According to the World Health Organization (WHO), food-borne diseases caused by pathogenic...

AI Technical Summary

Problems solved by technology

[0021] To sum up, with the continuous development of science and technology, more and more food comb biological detection technologies have been developed, but the traditional detection methods of foodborne pathogens are not only cumbersome to operate, but also take a long time to detect. It takes 5-7 days for culture and identification, which cannot meet the requirements of rapid detection in terms of time

Furthermore, the identification of bacteria is a time-consuming and complicated task. For example, the detection of Campylobacter jejuni requires 15 kinds of culture media and more than 20 steps of size, and the operation is very complicated.

Moreover, the relative instability of the biochemical characteristics of bacteria also brings great difficulties and uncertainties to the identification of bacteria. At the same time, due to factors such as unstable quality of some biochemical reagents, serum, etc. Inaccurate

At present, the commonly used bacterial identification cards at home and abroad, such as API, although the quality is stable, but there are many human factors in the identification, and the results of different operators are often different. The automatic identification of bacteria has good reproducibility, but it is limited to a few A biochemical reaction, the identification between similar strains is particularly difficult, and the detection efficiency is low

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