30 results about "Molecular array" patented technology

The invention relates to the technical field of biological detection, and particularly discloses a marker combination for detecting Alzheimer's disease, which comprises the following markers: beta amyloid protein 1-40, beta amyloid protein 1-42, phosphorylated Tau217 protein, phosphorylated Tau181 protein, phosphorylated Tau231 protein, neurofilament light chain protein and colloidal fiber acidic protein. The invention also discloses application of the marker combination for detecting the Alzheimer's disease in single molecule detection. The application comprises the following steps: S1, preparing standard solutions with different concentrations of the markers; s2, detecting the standard solutions with different concentrations of each marker by adopting a single molecule array analysis technology, and counting corresponding single molecule signal numbers; and S3, drawing a standard curve of each marker according to the single molecule signal numbers corresponding to the standard solutions with different concentrations of each marker. The method has the advantages that the overall performance of the AD disease biomarker combination can be accurately evaluated, and the reliability and accuracy of AD disease markers in clinical application are improved.

The invention relates to the field of monomolecular immunodetection chips, and particularly discloses a preparation method of a monomolecular array chip of a composite resin substrate, which comprises the following steps: S1, pretreating a glass substrate to obtain a pretreated substrate; s2, the composite resin substrate material is heated to be molten, the temperature is maintained so that the composite resin substrate material can be kept in a molten state, and molten resin is obtained; s3, the surface of the pretreated substrate is evenly coated with molten resin, the coating thickness is 30-75 microns, and a coated substrate is obtained; and S4, pressing the nanoimprint mold on the surface of the coating of the coating substrate, and after the nanoimprint mold is cooled to 25 DEG C or below, removing the nanoimprint mold to obtain an imprint substrate. The single-molecule array chip has the advantages that the manufacturing cost of the single-molecule array chip can be reduced and the flexibility of the chip can be improved on the premise of ensuring high-sensitivity detection.

An assembly for securing a film to an object is provided. The assembly may comprise a lid comprising a surface configured to receive the film, where the surface comprises a plurality of vent openings through which positive pressure or vacuum may be applied to the film. The application of a vacuum may flatten the film against the surface. The assembly may also comprise a base comprising a cavity configured to receive and support a tray, and at least two receptacles configured to receive at least two pegs of the lid. The film may comprise a molecular array which may be immobilized on one side of the film.

Provided in some aspects are methods for light-controlled in situ surface patterning of a substrate. Compositions such as nucleic acid arrays produced by the methods are also disclosed. In some embodiments, a method disclosed herein comprises using photoresist for photocontrollable hybridization and / or ligation of nucleic acid molecules, wherein photoresist removal allows hybridization and / or ligation of nucleic acid molecules at the exposed area. A large diversity of barcodes can be created in molecules on the substrate via sequential rounds of light exposure, hybridization, and ligation.

The invention relates to high throughput nucleic acid sequencing with a single molecule sensor array. Embodiments of single molecule array sequencing (SMAS) devices and systems are disclosed herein. Each sensor in a sensor array of the SMAS device is capable of detecting a label attached to a nucleotide incorporated into a single nucleic acid strand that binds to a respective binding site. Each sensor may detect a single label (e.g., fluorescent, magnetic, organometallic, charged molecules, etc.) attached to the incorporated nucleotides. Also disclosed are methods of highly tunable nucleic acid (e.g., DNA) sequencing based on synthetic sequencing (SBS) of various examples of colonized amplified DNA immobilized on such SMAS devices using the SMAS devices and systems. Also disclosed are error correction methods that mitigate errors generated in sequencing individual nucleic acid strands (e.g., detecting or not detecting error markers).

The invention relates to the field of monomolecular immunodetection chips, and particularly discloses a preparation method of a monomolecular array chip based on a functionalized epoxy resin substrate, and the preparation method comprises the following steps: S1, pretreating a glass substrate to obtain a pretreated substrate; s2, the resin substrate material is heated to be molten, the temperature is maintained so that the resin substrate material can be kept in a molten state, and molten resin is obtained; s3, the surface of the pretreated substrate is evenly coated with molten resin, the coating thickness is 20-60 microns, and a coated substrate is obtained; and S4, pressing the nanoimprint mold on the surface of the coating of the coating substrate, and after the nanoimprint mold is cooled to 25 DEG C or below, removing the nanoimprint mold to obtain an imprint substrate. The single-molecule array chip has the advantages that the manufacturing cost of the single-molecule array chip can be reduced and the flexibility of the chip can be improved on the premise of ensuring high-sensitivity detection.

Provided in some aspects are methods for light-controlled in situ surface patterning of a substrate. Compositions such as nucleic acid arrays produced by the methods are also disclosed. In some embodiments, a method disclosed herein comprises using photoresist for photocontrollable hybridization and / or ligation of nucleic acid molecules, wherein photoresist removal allows hybridization and / or ligation of nucleic acid molecules at the exposed area. A large diversity of barcodes can be created in molecules on the substrate via sequential rounds of light exposure, hybridization, and ligation.

The present invention relates to the field of single-molecule immunoassay chips, and specifically discloses a method for preparing a single-molecule array chip with a composite resin substrate, comprising the following steps: S1, pretreating a glass substrate to obtain a pretreated substrate; S2, heating the composite resin substrate material to melting and maintaining the temperature to keep the composite resin substrate material in a molten state to obtain molten resin; S3, uniformly coating the molten resin on the surface of the pretreated substrate with a coating thickness of 30-75 μm to obtain a coated substrate; S4, pressing a nanoimprint mold onto the coating surface of the coated substrate, and after cooling to below 25°C, removing the nanoimprint mold to obtain an imprinted substrate. Its advantages are: it can reduce the manufacturing cost of the single-molecule array chip and improve the chip flexibility while ensuring high-sensitivity detection.

Provided in some aspects are methods for light-controlled in situ surface patterning of a substrate comprising a step of blocking or ablating oligonucleotide molecules in a boundary region separating a plurality of spot regions, and attaching oligonucleotides to oligonucleotide molecules in a first one or more of the spot regions.

Methods of collecting diffraction patterns from a microcrystal having an ordered array of a molecule are disclosed, which include using an exposure rate of at most 0.02 electrons per square angstrom per second on the microcrystal and using a direct electron detector to record electron diffraction patterns. Also disclosed are methods of determining a structural model for a molecule, identifying a material present in a trace amount within a sample, identifying a polymorph, and identifying the stereochemistry of a molecule.

The invention relates to the technical field of a cesium feed-in system in a magnetic confinement nuclear fusion negative neutral beam system, in particular to an optimization method for DSMC numerical simulation of a cesium feed-in system based on CUDA parallel. According to the technical scheme, the method comprises the following steps: setting a molecular initial state at a host end, and preparing initial conditions for analog computation; allocating a memory space for the molecular array and the related data structure at the equipment end; molecular motion is calculated in parallel through a GPU at an equipment end, and collision between molecules and a wall surface is processed. According to the method, the simulation efficiency is improved to the minute level through a CUDA parallel computing architecture, a grid-based molecular index and memory access mechanism is optimized, the statistical reliability is guaranteed by adopting a thread-independent random number generator, near-vacuum molecular index is accurately restored by means of a decoupled physical model, and the statistical reliability is improved. Therefore, an efficient and practical analysis tool is provided for cesium feed-in system engineering design on the premise that the numerical precision is guaranteed.

The invention relates to methods of determining whether a subject has, or is at risk of developing, a neurodegenerative disorder using ultra-sensitive techniques, in particular a single-molecular array detection method.

Provided in some aspects are methods for light-controlled in situ surface patterning of a substrate comprising a step of blocking or ablating oligonucleotide molecules in a boundary region separating a plurality of spot regions, and attaching oligonucleotides to oligonucleotide molecules in a first one or more of the spot regions.

Embodiments of single molecule array sequencing (SMAS) devices and systems are disclosed herein. Each sensor in the sensor array of the SMAS device is capable of detecting a label attached to a nucleotide incorporated into a single nucleic acid chain bound to a respective binding site. Each sensor can detect a single label (e.g., fluorescent, magnetic, organic metal, charged molecule, etc.) attached to the incorporated nucleotide. Also disclosed are methods for highly adjustable nucleic acid (e.g., DNA) sequencing based on synthetic sequencing (SBS) of multiple examples of cloned amplified DNA fixed on such a SMAS device using a SMAS device and system. Also disclosed are error correction methods that mitigate errors (e.g., detection or non-detection of erroneous labels) generated in sequencing individual nucleic acid chains.

The present disclosure relates to polymer coatings covalently attached to the surface of a substrate and the preparation of the polymer coatings, such as poly(N-(5-azidoacetamidylpentyl)acrylamide-co-acrylamide) (PAZAM), in the formation and manipulation of substrates, such as molecular arrays and flow cells. The present disclosure also relates to methods of preparing a substrate surface by using beads coated with a covalently attached polymer, such as PAZAM, and the method of determining a nucleotide sequence of a polynucleotide attached to a substrate surface described herein.

The invention belongs to the technical field of intelligent materials, and particularly relates to a response type porous material, a preparation method and application thereof, an intelligent film and a preparation method and application thereof. The response type porous material is a long-range ordered crystalline porous material and is stable in structure, ordered response groups / fragments are arranged in an ion channel, an ordered molecular array is formed, ion transmission between an external stimulation signal and a response site is more efficient, and the response type porous material can be applied to the fields of acid-base, temperature, solvent, ion recognition and the like. And the charge density in the structure and the size of the nano-fluid channel are stimulated to change, so that excellent selectivity and permeability are achieved, ion flow in the ion channel can be intelligently adjusted, a fine ion transmission function equivalent to that of an organism is realized, ions are selectively transmitted, specific ions are rapidly conducted, and ion flow is responsively controlled.

The application belongs to the technical field of intelligent materials, and particularly relates to a responsive porous material, a preparation method and application thereof, and an intelligent membrane, a preparation method and application thereof. The responsive porous material provided by the application is a long-range ordered crystalline porous material, which is stable in structure, has ordered response groups / fragments in the ion channel, forms an ordered molecular array, makes ion transmission between an external stimulation signal and a response site more efficient, changes the charge density and nanofluid channel size in the stimulation structure in acid-base, temperature, solvent and ion recognition scenes, has excellent selectivity and permeability, can intelligently adjust ion flow in the ion channel, realizes a fine ion transmission function comparable to a biological body, selectively transmits ions, rapidly conducts specific ions, and responsively controls ion flow.

Provided in some aspects are methods for light-controlled in situ surface patterning of a substrate. Compositions such as nucleic acid arrays produced by the methods are also disclosed. Further provided in some aspects are methods for light-controlled in situ refinement of nucleic acid array feature boundaries. In some embodiments, a method disclosed herein comprises using photoresist for photocontrollable removal, blocking, and / or inactivation of nucleic acid molecules at feature boundaries. A large diversity of barcodes can be created in molecules on the substrate via sequential rounds of light exposure, hybridization, ligation, and further interceded and / or followed by one or more rounds of photocontrollable refinement of array feature boundaries, to improve spatial array resolution and sensitivity.

Provided in some aspects are methods for light-controlled in situ surface patterning of a substrate. Compositions such as nucleic acid arrays produced by the methods are also disclosed. Further provided in some aspects are methods for light-controlled in situ refinement of nucleic acid array feature boundaries. In some embodiments, a method disclosed herein comprises using photoresist for photocontrollable removal, blocking, and / or inactivation of nucleic acid molecules at feature boundaries. A large diversity of barcodes can be created in molecules on the substrate via sequential rounds of light exposure, hybridization, ligation, and further interceded and / or followed by one or more rounds of photocontrollable refinement of array feature boundaries, to improve spatial array resolution and sensitivity.

Provided in some aspects are methods for light-controlled in situ surface patterning of a substrate. Compositions such as nucleic acid arrays produced by the methods are also disclosed. In some embodiments, a method disclosed herein comprises using photoresist for photocontrollable hybridization and / or ligation of nucleic acid molecules, wherein photoresist removal allows hybridization and / or ligation of nucleic acid molecules at the exposed area. A large diversity of barcodes can be created in molecules on the substrate via sequential rounds of light exposure, hybridization, and ligation.

The invention relates to a DOA (direction of arrival) estimation method based on a genetic algorithm combined with a molecular array. The DOA estimation method comprises the following steps: optimizing an array by adopting the genetic algorithm, generating a candidate solution and calculating a target function; calculating a digital beam by using the optimal weight vector, dividing the whole array into four sub-arrays according to the physical layout of the antenna array, improving the channel weighting precision, and reducing the angular resolution to be below # imgabs0 #; a sub-array division method is adopted to suppress a grating lobe effect, and the signal quality of a radar system is optimized; dML angle solution is carried out on each sub-array, a target azimuth angle and a target pitch angle are directly calculated from weighted channel data to adapt to a non-uniform array structure, and a DOA angle is obtained through peak detection; according to the method provided by the invention, the flexibility and the precision of the angulation process are improved, the grating lobe effect is effectively inhibited, and the problems of precision and stability of a traditional method under a non-uniform array condition are solved.

The present invention relates to the field of single-molecule immunoassay chips, and specifically discloses a preparation method of a single-molecule array chip based on a functionalized epoxy resin substrate, comprising the following steps: S1, pretreating a glass substrate to obtain a pretreated substrate; S2, heating a resin substrate material to melting and maintaining the temperature to keep the resin substrate material in a molten state to obtain molten resin; S3, uniformly coating the molten resin on the surface of the pretreated substrate with a coating thickness of 20-60 μm to obtain a coated substrate; S4, pressing a nanoimprint mold onto the coating surface of the coated substrate, and after cooling to below 25°C, removing the nanoimprint mold to obtain an imprinted substrate. Its advantages are: it can reduce the manufacturing cost of the single-molecule array chip and improve the chip flexibility on the premise of ensuring high-sensitivity detection.

The present invention relates to the field of single-molecule immunoassay chips, and specifically discloses a single-molecule array chip based on high-entropy alloy plasmons and a preparation method thereof. The production raw materials include a substrate chip and a high-entropy alloy coating material. The high-entropy alloy coating material is composed of a noble metal composite material and a synergistic composite metal material in a mass ratio of 1:0.12 to 3.75. The noble metal composite material is composed of gold and silver in a mass ratio of 1:0.08 to 3. The synergistic composite metal material is composed of ruthenium, palladium, and platinum in a mass ratio of 1:0.2 to 1.5:0 to 1.2. Its advantages are: it can significantly improve the signal-to-noise ratio of the single-molecule array chip and further improve the accuracy and reliability of this technology in single-molecule immunoanalysis.

The present invention relates to the field of single-molecule immunoassay chips, and specifically discloses a single-molecule array chip based on molybdenum disulfide doping and a preparation method thereof. The production raw materials include a substrate chip and a molybdenum disulfide doping coating material; the molybdenum disulfide doping coating material is composed of a synergistic metal material and molybdenum disulfide according to a mass ratio of 1:0.01 to 0.8; the synergistic metal material includes ruthenium. Its advantages are: it can significantly improve the signal-to-noise ratio of the single-molecule array chip, and further improve the accuracy and reliability of this technology in single-molecule immunoanalysis.

The invention relates to the field of monomolecular immunodetection chips, and particularly discloses a preparation method of a monomolecular array chip with a polycarbonate resin substrate, and the preparation method comprises the following steps: S1, pretreating a glass substrate to obtain a pretreated substrate; s2, the resin substrate material is heated to be molten, the temperature is maintained so that the resin substrate material can be kept in a molten state, and molten resin is obtained; s3, the surface of the pretreated substrate is evenly coated with molten resin, the coating thickness is 30-75 microns, and a coated substrate is obtained; and S4, pressing the nanoimprint mold on the surface of the coating of the coating substrate, and after the nanoimprint mold is cooled to 25 DEG C or below, removing the nanoimprint mold to obtain an imprint substrate. The single-molecule array chip has the advantages that the manufacturing cost of the single-molecule array chip can be reduced and the flexibility of the chip can be improved on the premise of ensuring high-sensitivity detection.

The present invention relates to the field of single-molecule immunoassay chips, specifically disclosing a multi-metal plasmon-functionalized single-molecule array chip and its preparation method. The production raw materials include a substrate chip and a multi-metal coating material. The multi-metal coating material comprises a composite noble metal coating material and a molybdenum disulfide-doped coating material in a mass ratio of 1:0.1-5; the noble metal coating material comprises gold and silver in a mass ratio of 5-10:1; and the molybdenum disulfide-doped coating material comprises a synergistic metal material and molybdenum disulfide in a mass ratio of 1:0.01-0.8; the synergistic metal material includes ruthenium. Advantages of this method include significantly improving the signal-to-noise ratio of the single-molecule array chip, further enhancing the accuracy and reliability of this technology in single-molecule immunoassays.

The invention discloses a plasmon supermolecule-based distributed Bragg reflector resonant cavity, which comprises a plasmon supermolecule distributed Bragg reflector and a dielectric cavity, and is characterized in that the plasmon supermolecule distributed Bragg reflector takes a gold nanoparticle supermolecule array layer and a dielectric layer as repetitive units, the number of the repetitive units is 1-100, the gold nanostructure supramolecular unit is formed by densely arranging N gold nanoparticles, and the distributed Bragg reflector resonant cavity is of a sandwich structure composed of two plasmon supramolecular distributed Bragg reflectors and a medium cavity. The resonant cavity has excellent performances of small cavity length, high quality factor, deep resonant depth and the like. Therefore, the distributed Bragg reflector resonant cavity of the plasmon supermolecule array can effectively improve the performance of a photoelectric device and reduce the size of the device at the same time.

The present invention discloses a distributed Bragg reflector resonant cavity based on plasmonic supramolecular structures, comprising a plasmonic supramolecular distributed Bragg reflector and a dielectric cavity. The plasmonic supramolecular distributed Bragg reflector comprises a gold nanoparticle supramolecular array layer and a dielectric layer as repeating units, with the number of repeating units ranging from 1 to 100. The gold nanostructured supramolecular unit is composed of N densely packed gold nanoparticles. The distributed Bragg reflector resonant cavity is a sandwich structure consisting of two plasmonic supramolecular distributed Bragg reflectors and a dielectric cavity. The resonant cavity exhibits excellent properties such as a short cavity length, a high quality factor, and a deep resonance depth. Therefore, this distributed Bragg reflector resonant cavity with a plasmonic supramolecular array can effectively improve the performance of optoelectronic devices while reducing their size.

The present invention relates to the field of single-molecule immunoassay chips, specifically disclosing a precious metal signal-enhanced plasma single-molecule array chip and its preparation method. The raw materials for production include a substrate chip and a composite coating material; the composite coating material comprises a precious metal composite material and a synergistic composite metal material in a mass ratio of 1:0.03-2; the precious metal composite material comprises gold and platinum in a mass ratio of 1:0.01-0.5; and the synergistic composite metal material comprises ruthenium and palladium in a mass ratio of 1:0.2-1.5. This method has the advantage of significantly improving the signal-to-noise ratio of the single-molecule array chip, further enhancing the accuracy and reliability of the technology in single-molecule immunoassay analysis.