106results about How to "Increase positive charge" patented technology

A conductive polymer is formed enzymatically in the presence of a polynucleotide template. The method includes combining at least one redox monomer with a polynucleotide template and a redox enzyme, such as horseradish peroxidase, to form a reaction mixture. The monomer aligns along the template before or during the polymerization. Therefore, the polynucleotide template thereby affects the molecular weight and conformation of the conductive polymer. When the conductive polymer is complexed to a polynucleotide duplex, the conformation of the polynucleotide duplex can be modulated by changing the oxidation state of the conductive polymer.

Methods of forming electrostatically charged gelatin are provided. Gelatin is extracted by an acid extraction method (12) thereby producing a gelatin dispersion (14), the pH of which is adjusted to the isoelectric point of the gelatin. The pH of the gelatin dispersion (14) is then further adjusted (22) so as to cause the gelatin to assume an electrostatic charge.

There is described a method of promoting the attachment, survival and / or proliferation of a stem cell in culture, the method comprising culturing a stem cell on a positively-charged support surface. There are also provided a cell composition prepared according to the method of the invention.

A device and method for purifying waste water and stormwater flows by passage through a filter, which can be manufactured in various configurations. The filter has a broad range of thickness ranging from 1 mm to 20 meters. The filter is comprised of fibers from a melt of composition of 50% silicon dioxide, 15% aluminum oxide, 15% calcium oxide, 10% magnesium oxide, and other various oxides at lower percentages. At least the majority of fibers having a mean diameter of 4 microns and a mean length of 3 mm. The fiber solids of content of the material are at most 35% of the volume at a flow rate greater than 1 inch per hour to remove various pollutants from the water flow.

A planar, rigid substrate made from a porous, inorganic material coated with cationic polymer molecules for attachment of an array of biomolecules, such as DNA, RNA, oligonucleotides, peptides, and proteins. The substrate has a top surface with about at least 200 to about 200,000 times greater surface area than that of a comparable, non-porous substrate. The cationic polymer molecules are anchored on the top surface and in the pores of the porous material. In high-density applications, an array of polynucleotides of a known, predetermined sequence is attached to this cationic polymer layer, such that each of the polynucleotide is attached to a different localized area on the top surface. The top surface has a surface area for attaching biomolecules of approximately 387,500 cm2 / cm2 of area (˜7.5 million cm2 / 1×3 inch piece of substrate). Each pore of the plurality of pores in the top surface of the substrate has a pore radius of between about 40 Å to about 75 Å. Not only does the cationic coating in and over the pores of the substrate greatly increase the overall positive charge on the substrate surface, but also given the size of the pores provides binding sites to which biomolecules can better attach.

The present invention provides for a static charge generating assembly. The static charge generating assembly is comprised of: (a) a support structure built from a plurality of friction fit building elements; (b) positive and negative static charge generating wheels; (c) a belt wrapped around the static charge generating wheels; (d) a power source electrically coupled to a driving means for rotating at least one of the static charge generating wheels and the belt at a preselected rotational speed; and (e) positive and negative electrodes secured to the support structure adjacent to the belt. Each of the electrodes is mounted on at least one of the friction fit building elements positioned adjacent to a static charge generating wheel operable to collect static charge. Separate positive and negative static charge collectors are used for storing the negative and positive static charges from the electrodes.

A protection device and a method for manufacturing integrated circuit devices protect against plasma charge damage, and related charge damage during manufacture. The protection device comprises a dynamic threshold, NMOS / PMOS pair having their respective gate terminals coupled to the semiconductor bulk in which the channel regions are formed. With proper metal connection, the structure protects against plasma charge damage on the integrated circuit device during manufacture, and can also be operated to protect against abnormal voltages during operation of the circuit.

The invention discloses a method for preparing high-concentration nano aluminum hydroxide aqueous dispersion, which comprises the following steps of: (1) adding monoacid serving as a dispersing agent into deionized water, and regulating the pH value to be 2 to 6.5; (2) adding nano aluminum hydroxide powder with stirring for dispersing; and (3) adding a surface modifier to modify the surface of the nano aluminum hydroxide with stirring, and finally preparing the high-concentration nano aluminum hydroxide aqueous dispersion with the concentration of between 30 to 66 weight percent, wherein the step (2) and the step (3) are simultaneously or alternately performed. The prepared nano aluminum hydroxide aqueous dispersion has the characteristics of high concentration, low viscosity, high stability and the like, and is particularly suitable to be applied to blotting materials.

The invention discloses a high-performance forward osmosis membrane as well as a preparation method and an application thereof. The method comprises steps as follows: the upper surface of a base membrane is soaked in a mixed water solution of PAMAM (polyamidoamine) dendrimer and MPD (m-phenylenediamine) for 2-5 min, then, the base membrane is taken out, and the solution on the surface of the basemembrane is sucked dry with filter paper; the upper surface of the base membrane is continuously soaked in a TMC (trimesoyl chloride) solution for 1-3 min, then, the solution is discarded, and the high-performance forward osmosis membrane is obtained. In the prepared high-performance forward osmosis membrane, PAMAM can increase the relative free volume and the hydrophilcity of a PA (polyamide) layer, so that the water flux of the forward osmosis membrane is improved; besides, free amido on PAMAM in the polyamide layer can adsorb heavy metal ions and increase the positive charge property of themembrane, so that the repulsive interaction on the heavy metal ions in water is enhanced, and a promoting function is produced on the forward osmosis membrane for removing the heavy metal ions in water.

The invention provides reduction-responsive hyperbranched poly-beta-amino ester with efficient gene delivery capability and a preparation method and application thereof. The polymer is polymerized byan A2 + B3 + C2 Michael addition method so that the polymer has a hyperbranched structure. Compared with a linear structure, the branched structure can enhance the interaction between the polymer andnucleic acid molecules, significantly improve the gene condensation ability of the polymer, and increase cellular uptake by enhancing the interaction with cell membranes. The main chain of the polymerhas a reduction-responsive group (disulfide bond), and under the action of GSH in damaged vascular endothelial cells, poly-beta-amino ester can be quickly degraded in cytoplasm to release an entrapped gene drug (ICAM-1 siRNA) so that efficient transfection of genes is realized, and meanwhile, the toxicity of the material is reduced. Due to the properties of the poly-beta-amino ester, the poly-beta-amino ester has a huge development prospect in biomedical materials, especially in the field of gene delivery.

The invention discloses a nano manganese oxide composite material as well as a preparation method and an application of the composite material in anion pollutant processing, and belongs to the field of a nano composite material of an environmental function. The composite material is formed by utilizing an anion exchange resin to immobilize hydrated manganese oxide particles, and the loading manganese amount is 2-40%. The anion exchange resin is a strongly basic anion exchange resin of a functional group of positive charge. The particle diameter of the hydrated manganese oxide particle is 2-50 nm. The average particle diameter of the composite material is 0.5-1.5 mm, the specific surface area of the composite material is 5-30 m<2> / g, the pore volume of the composite material is 0.005-0.4 cm<3> / g, and the zero charge point of the composite material is 9.5-11. The manganese oxide is loaded on the nanoporous material of which the surface is bound with the functional group of positive charge, so that the surface electrical property of the manganese oxide is controlled, and the adsorptive property of the composite material to the anion pollutants is improved.

The invention relates to a method for removing bromide ions out of bromide ion-containing drinking water. The method is characterized by comprises the following steps of: before the bromide ion-containing drinking water is treated by using an ozone-biologic activated carbon process, adding 0.5-1mg / L potassium permanganate into raw water, preoxidizing for 3-5 minutes, and then sequentially adding 12-18mg / L polymeric aluminum by Al and 8-12mg / L ferric trichloride by Fe for coagulating, wherein the mass concentration ratio of polymeric aluminum to ferric trichloride is 3:2. The method is simple to operate and safe and reliable, the bromide ion content (100-100mu g / L) of the raw water is reduced by 45-70%, and the purpose of controlling the bromine disinfection by-product generation in the later ozonation bromate and disinfection period is reached; therefore, the method has wide application prospects.

A liquid developer contains, for example, a nonpolar liquid, thermoplastic resin, optional colorant, and an inorganic filler

A conductive polymer is formed enzymatically in the presence of a polynucleotide template. The method includes combining at least one redox monomer with a polynucleotide template and a redox enzyme, such as horseradish peroxidase, to form a reaction mixture. The monomer aligns along the template before or during the polymerization. Therefore, the polynucleotide template thereby affects the molecular weight and conformation of the conductive polymer. When the conductive polymer is complexed to a polynucleotide duplex, the conformation of the polynucleotide duplex can be modulated by changing the oxidation state of the conductive polymer.

The application provides polypeptide Pv26-3 with antimicrobial activity, belonging to the technical field of genetic engineering drugs. In the polypeptide Pv26-3, tryptophan W replaces the 7th, 11th,21st and 25th sites in the amino acid sequence of a parent molecule Pv26, and lysine K replaces amino acids at the 1st, 12th, 20th and 24th sites, thereby enhancing the hydrophobicity and positive charge of the parent molecule Pv26. Since a hydrophobic group plays a key role in the process of inserting antimicrobial peptide into a cell membrane, the improvement of hydrophobicity is beneficial to the antimicrobial effect of the antimicrobial peptide; the antimicrobial peptide is bonded to the membrane by electrostatic interaction, so as to promote the combination of the hydrophobic group into the membrane, and then the membrane structure is destroyed to cause cell death, so that positive charge is the key factor affecting the binding of the antimicrobial peptide and the membrane; by improving hydrophobicity and positive charge, the binding ability of the antimicrobial peptide and the membrane can be enhanced, the antimicrobial activity and antibacterial effect of antimicrobial peptide can be improved, and at the same time, the action concentration of antimicrobial peptide is lowered.

The present invention provides methods and compositions for attenuating expression of a target gene in vivo. In general, the method includes administering RNAi constructs (such as small-interfering RNAs (i.e., siRNAs) that are targeted to particular mRNA sequences, or nucleic acid material that can produce siRNAs in a cell), in an amount sufficient to attenuate expression of a target gene by an RNA interference mechanism. In particular, the RNAi constructs include one or more modifications to improve serum stability and cellular uptake and to avoid non-specific effect.

The invention relates to a preparation method and application of a polyacrylamide / keramite nanotube composite papermaking filler. The preparation method comprises the following steps of: adding a keramite nanotube into aqueous solution of acrylamide monomer or mixed solution of water and ethanol of acrylamide, then loading acrylamide monomer molecules to a cavity of the keramite nanotube by using vacuum negative pressure, and preparing the keramite nanotube / acrylamide composite monomer; and then through a free radical solution polymerization reaction, polymerizing the keramite nanotube / acrylamide composite monomer, and a polyacrylamide molecule chain penetrating through the keramite nano cavity, so as to prepare the olyacrylamide / keramite nanotube composite papermaking filler. The olyacrylamide / keramite nanotube composite papermaking filler is added into the papermaking pulp, so that the amount of fillers in the paper can be increased; and the effect on physical strength of the paper caused by adding the fillers can be reduced.

The invention discloses a sewage treatment flocculant. The sewage treatment flocculant comprises the following raw materials in parts by weight: 105-115 parts of xylogen, 75-82 parts of propyl aldehyde, 26-29 parts of diethylenetriamine, 35-38 parts of sodium hydroxymethanesulfonate, 580-600 parts of isopropanol, 12-15 parts of 3-chloro-2-hydroxypropyl trimethyl ammonium chloride, 25-28 parts of polymeric aluminum ferric sulfate, 12-15 parts of alum, 23-26 parts of polymeric ferric chlorosulfate and 25-28 parts of polyacrylic acid. The invention further discloses a preparation method of the sewage treatment flocculant. The sewage treatment flocculant is applied to the treatment of heavy metal sewage, the flocculating performance is good, the removal effect of heavy metals is good, and the scaling can be prevented.

The invention relates to the technical field of leather making techniques and provides a wet-state dyeing finishing and fixation method for leathers based on a zirconium-aluminum-titanium complex. The zirconium-aluminum-titanium complex is added in a later period of dyeing and fat-liquoring; the zirconium-aluminum-titanium complex is a multi-metal cation tanning agent which may combine with leather fibers so as to increase positive charge on leather surface, to improve tinting strength of the leather surface and color brightness and to basically solve the shading problem, color fullness of cationic crust leathers is improved, consumption of dyes and formic acid in traditional fixation methods is lowered, dyeing rate and fastness of dyes are increased, the content of dyes in spent dye liquor is further decreased, wastewater treatment is easier, and a leather making process is greener.

A liquid developer containing a liquid, colorant, and a wax charge acceptance additive. When free of colorant, the developer is considered colorless or slightly colored.

The present invention relates to a catalyst for preparation of styrene and ethylbenzene by a side chain alkylation reaction of toluene and application thereof, and mainly solves the problems of low utilization rate of a C1 raw material, low conversion rate of toluene and poor stability of a current catalyst used for the side chain alkylation reaction of toluene to produce ethylbenzene and styrene. According to the invention, the catalyst uses a modified X or Y molecular sieve as an active component, and at least one of group IIIA elements, at least one of VII B elements, at least one of alkali metal elements, at least one of the alkaline earth elements and at least one of lanthanide elements as modified components. A technical scheme, in which toluene and C1 raw materials contact with the catalyst to be subjected to a reaction to produce ethylbenzene and styrene, well solves the problems, and the catalyst can be used in the industrial production of preparation of ethylbenzene and styrene by the side chain alkylation reaction of toluene and methanol.

The invention relates to antibacterial hydrogel. The antibacterial hydrogel consists of unsaturated polyester, dimethyl diallyl ammonium chloride, di(ethylene glycol) ethyl ether acrylate and photoinitiator, wherein the weight of the dimethyl diallyl ammonium chlorid is 1 time to 2 times of the weight of the unsaturated polyester, the weight of the di(ethylene glycol) ethyl ether acrylate is 2 times to 4 times of the weight of the unsaturated polyester, and the weight of the photoinitiator is 0.1 time to 0.5 time of the weight of the unsaturated polyester. The unsaturated polyester is initiated by methoxypolyethylene glycols, and is obtained by performing ring-opening polymerization on succinic anhydride, ethylhexyl glycidyl ether and glycidyl methacrylate. The antibacterial hydrogel disclosed by the invention is high in hydrophilicity and antibacterial performance, and long in antibacterial time.

The invention relates to a modified composite fiber membrane and a layer-by-layer self-assembly method and application thereof. The layer-by-layer self-assembly method of the modified composite fibermembrane includes the following steps that a micro / nano high-molecular fiber membrane is prepared; the surface of the micro / nano high-molecular fiber membrane is subjected to plasma modification, anda bottom plate is obtained; a polyelectrolyte A and a polyelectrolyte B which have opposite charges are alternately assembled on the bottom plate with a soaking layer-by-layer self-assembly technologyuntil the required layer number reaches, and the modified composite fiber membrane is obtained. Compared with a traditional layer-by-layer self-assembly method, the layer-by-layer self-assembly method of the modified composite fiber membrane is easy and convenient to operate and high in efficiency, materials which are originally hydrophobic and are not suitable for the fields of biological medicine, environmental protection and the like can have good biocompatibility, the performance of promoting cell reproduction and the like, a selection range of the bottom plate is remarkably widened, andmeanwhile an application range of the layer-by-layer self-assembly method of the modified composite fiber membrane is widened.

Production of heating agent for tumor magnetic thermal therapy is carried out by preparing Fe3O4 nanometer particles from wetting chemical coprecipitation, regulating reactant proportion and increasing volume of reacting system to regulate size of Fe3O4 nanometer particles, washing reactant for 3-4 times from magnetic separation, diluting to 2-4g / lwater based Fe3O4 magnetic liquid from water, stirring at 90-100íÒC and pH=2-3, inducing into air, oxidizing for 4-5hrs, forming water based gamma-Fe3O4 magnetic liquid, cooling, adding into glutamic acid 5-10wt%, surface modifying, keeping pH=3+-0.1 and stirring for 4-5hrs, washing for 3-4 times from magnetic separation, obtaining stabilized nanometer heating agent, and dissolving into different concentration 0-20g / l.

The invention discloses an efficient sewage treatment flocculant which comprises the following raw materials in parts by weight: 100-110 parts of lignin, 70-80 parts of propionaldehyde, 25-30 parts of triethylene tetramine, 32-35 parts of formaldehyde sodium bisulfite addition compound, 600-620 parts of normal propyl alcohol, 10-12 parts of glycidyl trimethyl ammonium chloride, 23-26 parts of poly-ferric aluminum sulfate, 25-28 parts of PPFC, 4-8 parts of aluminium chloride and 22-26 parts of sodium polystyrene sulfonate. The invention further discloses a preparation method of the efficient sewage treatment flocculant. The efficient sewage treatment flocculant obtained through the preparation method is excellent in flocculating performance and heavy metal removal effect and can prevent scaling.

The invention relates to an in-situ polymerization method modified anion exchange resin as well as a preparation method and application thereof. The modified anion exchange resin is prepared by usingan anion exchange resin with a tertiary amine group [-N(CH3)2] as an organic framework, epoxy chloropropane and dimethylamine as a modifier and ethylenediamine as a cross-linking agent through modification. According to the modified anion exchange resin provided by the invention, the content of amino is increased, weak alkali groups are also increased, the Zeta potential of the modified anion exchange resin is also remarkably increased, and in addition, the isoelectric point of the modified anion exchange resin is 6.62 which is far greater than that of an unmodified resin material, so that theadsorption treatment function on Cr(VI) is greatly improved, and the modified anion exchange resin has the advantages of being stable in quality, excellent in adsorption property, low in cost, simplein solid-liquid separation, large in mechanical strength, high in adsorption capacity, good in regeneration effect, free of secondary pollution, and the like.

The invention discloses a magnetic adsorbent and a preparation method thereof, and belongs to the field of composite materials. According to the magnetic adsorbent, the cationic property of chitosan and the amphoteric structure and property of gelatin are combined, the magnetism of ferroferric oxide and the characteristic that CTAB serves as a cationic surface active agent are introduced, and a magnetic adsorption material with efficient adsorption and regeneration functions is prepared. The preparation method is simple, the magnetic adsorbent is easy to separate from a solution and not prone to loss, the separation process is time-saving and efficient, cyclic utilization can be achieved, and the magnetic adsorbent has wide application prospects in the aspect of dye adsorption.

The invention designs and synthesizes a fatty-acid-modified ultra-short-sequence antibacterial peptide analogue. The antibacterial peptide analogue is fatty-acid-modified polypeptide containing threeamino acids; arginine and tryptophan are arranged and combined in different modes to obtain an ultra-short sequence polypeptide containing three amino acids; amidation is performed on the C tail end of the ultra-short-sequence polypeptide and an N tail end of the ultra-short sequence polypeptide is modified with a C8-C18 fatty acid chain. The sequence is shorter, the design is simple, and the manufacturing cost is low. The in-vitro antibacterial experiments show that the ultra-short-sequence antibacterial peptide analogue has strong antibacterial activity on common strains, the antibacterial activity on gram-positive bacteria is superior to that on gram-negative bacteria. The ultra-short-sequence antibacterial peptide analogue has the broad application prospects in preparation of clinicalantibacterial drugs.