8 results about "Peptide array" patented technology

Provided herein is a peptide array comprising a plurality of flagellin peptides corresponding to highly conserved peptide regions. For example, the peptide array comprises a plurality of Lachnospiraceae flagellin peptides, which can be selected from a hinge region of Lachnospiraceae flagellin. Also provided is antibody of fragment thereof that binds to one or more of the plurality of the flagellin peptides in the peptide array. The peptide array and antibody are useful in determining an immunosignature from a biological sample from a subject. The immunosignature is useful in diagnosis of an immune-mediated disease, in monitoring progression of an immune-mediated disease, in identifying subjects susceptible to certain treatments, and in monitoring treatment response.

Provided herein is a method of generating a binding pattern from a sample obtained from a subject. In some embodiments, the method includes detecting binding of antibodies from the sample to an array of peptides that each comprise at least subsequences of B. burgdorferi protein sequences listed in SEQ ID NOS: 1-45 to produce a detected binding data set, and identifying one or more binding patterns in the detected binding data set. In some embodiments, the method includes classifying the binding pattern as indicative of the subject having Lyme disease, as indicative of the subject not having Lyme disease, or as indicative of the subject having a non-Lyme febrile disease. Provided also herein are other methods and related reaction mixtures, compositions, kits, devices, systems, and computer readable media.

Disclosed herein are formulations, substrates, and arrays. Also disclosed herein are methods for manufacturing and using the formulations, substrates, and arrays. Also disclosed are methods for identifying peptide sequences useful for diagnosis and treatment of disorders, and methods for using the peptide sequences for diagnosis and treatment of disorders, e.g., celiac disorder. In certain embodiments, substrates and arrays comprise a porous layer for synthesis and attachment of polymers or biomolecules.

The present disclosure relates generally to biomarkers and peptide arrays, and, more particularly, to a method of using a peptide array to identify biomarkers for an autoimmune disease such as, e.g., Celiac disease. Furthermore, a set of novel biomarkers for Celiac disease, having high sensitivity and specificity, are disclosed in addition to method of treatment using the novel biomarkers.

The application discloses a coating matrix for realizing in-situ synthesis and biological detection of high-density polypeptide arrays through one-step acid hydrolysis and application thereof, and relates to the technical field of biotechnology.The technical scheme points of the coating matrix are as follows: the structural composition of the coating matrix comprises a hydrophilic hydrogel matrix, and side-chain-protected polyamino acids are synthesized on the hydrophilic hydrogel matrix; the polyamino acids are one of hydroxyl group-protected serine [O'-X]S or hydroxyl group-protected threonine [O'-X]T, so that the coating matrix is modified by polymers with the structure of [O'-X]S)n or [O'-X]T)n; the coating matrix has low wettability to aprotic polar solvents used in polypeptide array synthesis, and the contact angle θ of the coating matrix is greater than 25°; the coating matrix has an amino group at the end, and can be chemically reacted with an electrophilic reagent; and the coating matrix can be treated by trifluoroacetic acid to remove the protecting group X, so as to be converted into a coating matrix with high wettability, and the contact angle θ of the coating matrix is less than 5°.

Disclosed herein are formulations, substrates, and arrays. Also disclosed herein are methods for manufacturing and using the formulations, substrates, and arrays. Also disclosed are methods for identifying peptide sequences useful for diagnosis and treatment of disorders, and methods for using the peptide sequences for diagnosis and treatment of disorders, e.g., celiac disorder. In certain embodiments, substrates and arrays comprise a porous layer for synthesis and attachment of polymers or biomolecules.

Disclosed herein are isolated compositions including at least 2 of mutant peptides selected from the group consisting of SEQ ID NOS: 1-149, or polypeptides comprising the mutant peptides; wherein the composition comprises mutant peptides encoded by 2 or more genes. Also disclosed are methods for personalized treatment of breast cancer involving creating a peptide array of mutant peptides comprising the mutations in protein-encoding regions of the high-frequency cancer genes or the exome in a subject and screening the peptide array with a biological sample from the subject to detect antibodies in the biological sample that bind to the array, to detect antigenic targets for therapy in treating the subject.

The present disclosure relates generally to biomarkers and peptide arrays, and, more particularly, to a method of using a peptide array to identify biomarkers for an autoimmune disease such as, e.g., celiac disease. Furthermore, a set of novel biomarkers for celiac disease, having high sensitivity and specificity, are disclosed in addition to method of treatment using the novel biomarkers.