130 results about "Protein Depositions" patented technology

Compositions and methods of treating a defect in a patient are disclosed, including expanding a culture of autologous cells in vitro to form cultured cells, collecting the cultured cells for introduction into the patient, and depositing the cultured cells with ancillary proteins.

The invention relates to methods of treating protein degradation disorders, such cellular proliferative disorders (e.g., cancer) and protein deposition disorders (e.g., neurodegenerative disorders). The invention provides methods and pharmaceutical compositions for treating these diseases using aggresome inhibitors or combinations of aggresome inhibitors and proteasome inhibitors. The invention further relates to methods and pharmaceutical compositions for treating multiple myeloma. New HDAC/TDAC inhibitors and aggresome inhibitors are also provided as well as synthetic methodologies for preparing these compounds.

The present invention relates to compositions and methods for the modulated release of one or more proteins or peptides. The composition is comprised of a biocompatible polymeric matrix, a protein and/or peptide, and a sparingly water-soluble or essentially insoluble particle. The protein is deposited by adsorption or some other mechanism onto the sparingly water-soluble biocompatible particle wherein the protein-particle combination is dispersed within the polymeric matrix. The deposition of the protein onto the particle acts to modulate the release of the protein or peptide from dosage forms including long-acting dosage systems.

The present invention relates to the use of direct-write lithographic printing of proteins and peptides onto surfaces. In particular, the present invention relates to methods for creating protein and peptide arrays and compositions derived therefrom. Nanoscopic tips can be used to deposit the peptide or protein onto the surface to produce a pattern. The pattern can be dots or lines having dot diameter and line width of less than 1,000 nm. The tips and the substrate surfaces can be adapted for the peptide and protein lithography.

The present invention relates to immunogenic but non-depositing-forming polypeptides or peptides homologous to amyloid β, prion, amylin or α-synuclein which can be used alone or conjugated to an immunostimulatory molecule in an immunizing composition for inducing an immune response to amyloid β peptides and amyloid deposits, to prion protein and prion deposits, to amylin and amylin deposits, to α-synuclein and deposits containing α-synuclein, or to polyglutamine repeats and deposits of proteins containing polyglutamine repeats. Described are also antibodies directed against such peptides, their generation, and their use in methods of passive immunization to such peptides and deposits.

The invention provides a method for detecting 31 common blood pressure-lowering and blood sugar-lowering drugs in blood by an ultra high performance liquid-mass spectrometry, and is characterized in that the method comprises the following steps: (1) confirmation of detected target compounds; (2) sample processing: processing a sample by an organic solvent protein-deposition method; (3) primary screening detection: carrying out primary screening detection by a multiple reaction monitoring (MRM) mode of the ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) in different periods of time; (4) reexamining of the sample which is positive by the primary screening detection in the step (3): after treating and concentrating the sample which is positive by the screening detection, carrying out UPLC-MS/MS detection; and optionally, (5) reexamining of the sample which is positive by the reexamining detection in the step (4): reexamining by a high performance liquid chromatography-high resolution time-of-flight tandem mass spectrometry (HPLC-QTOF). According to a fact that the drugs have different retention time in the set detection conditions, a method for sectional detection is creatively completed. The required time for totally completing the detection of 31 drugs can be within 11 minutes.

The invention provides a method for testing a spectrum of amino acid and organic acid in urine, wherein, a conventional protein deposition treatment is carried out on biological samples; capillary electrophoresis is adopted as a separate method; and a mass spectrometry detector which has good universality and high sensitivity is adopted as a detector. The method has the advantages of high precision and recovery, derivatization omission, simple operation, high sensitivity and good selectivity and universality and can simultaneously analyze 22 amino acids and 5 organic acids in the biological samples.

The invention provides a method for detecting 25-hydroxy-vitamin D through a high-sensitivity high-throughput ultra-performance liquid chromatography-tandem mass spectrometry device. The method comprises adding an internal standard substance of 25-hydroxy-vitamin D into a serum sample, adding a zinc sulfate solution and a methanol solution into the mixture so that protein deposition is realized, carrying out full mixing, adding a n-hexane solvent into the mixture, carrying out extraction, carrying out full mixing, carrying out centrifugation, taking the supernatant, carrying out nitrogen blowing drying, adding a compounded solution into the supernatant to obtain a sample to be detected, detecting the sample to be detected through an ultra-performance liquid chromatography-tandem quadrupole mass spectrometry device and carrying out quantitation through an internal standard curve method based on 25-hydroxy-vitamin D2 and/or 25-hydroxy-vitamin D3 quantitative and qualitative ion pair retention time as qualitative basis. The method has advantages of simple pre-treatment process, good specificity, good matrix interference resistance, short detection time, high throughput, high detection precision, high sensitivity and low cost.

The invention discloses a grass carp feed premix and application thereof. The grass carp feed premix comprises limited amino acid, compound vitamins and compound mineral salts. In the invention, plant raw materials are used as a main protein source, such as vegetable meals, cotton meals, soybean products, and the like; and if 1 percent of grass carp feed premix is added to prepare a submerged grass carp compound feed or an expanding feed for breeding grass carps, the protein deposition rate reaches 32 percent, the protein efficiency is improved to 1.9, the feed coefficient is reduced to 1.9, and the feed cost is saved y7.31-11.68 percent. The grass carp feed premix has significance for improving the utilization of the feed by the grass carps, promoting the growth, and the like.

The invention discloses a tilapia feed premix and an application thereof. The tilapia feed premix is composed of limiting amino acid, a vitamin complex and composite mineral salt. In the invention, plant raw materials such as rapeseed meal, cottonseed meal, soybean products and the like are taken as main protein sources, and 1% of the tilapia feed premix is added to the raw materials to prepare a tilapia submerged compound feed or an extruded feed for cultivating tilapia, which can achieve protein deposition rate up to 48%, improve protein efficiency to 2.85, lower feed coefficient to 1.35 and save feed cost by 12.20%-13.90%. The tilapia feed premix of the invention is of great practical significance for improvement of feed utilization of the tilapia, growth promotion and the like.

The invention relates to a feed capable of enhancing growth performance and protein deposition of a carp, solving the problem that protein deposition rate of the existing feed is low. The feed capable of enhancing growth performance and protein deposition of carp comprises the following components in parts by weight: 17-19 parts of fish meal, 29-40 parts of soybean meal, 32-45 parts of middlings, 0.8-1.1 parts of soybean lecithin, 2-3 parts of fish oil, 1-2 parts of soybean oil, 1.5-2.5 parts of monocalcium phosphate, 0.4-0.6 part of choline, 0.2-0.5 part of lysine, 0.1-0.25 part of sulfur-containing amino acid, 0.4-0.6 part of premix and 0.7-1.6 parts of alpha-ketoglutaric acid. The feed provided by the invention is applied to the field of carp cultivation.

The invention discloses a feed capable of reducing Yanan pig back-fat thickness and improving Yanan pig lean meat deposition rate. The feed comprises following components in percentage by weight: 55 to 75% of starch type feed, 15 to 20% of protein feed, 1 to 3% of grease, 8 to 15% of fiber feed, 1 to 5% of composite mineral elements and 0.3 to 0.6% of vitamin composite. Through years of practice, researches on nutrition requirement of Yanan pigs, nutrition and meat quality, and exploitation and utilization of novel feed resources, the components and ratio of the Yanan pig feed are scientifically adjusted, so that the utilization rate of the feed is increased, the immunity ability and the health conditions of the Yanan pigs are improved, the back-fat thickness of the Yanna pigs is reduced, and the protein deposition rate and lean meat ratio of the Yanan pigs are increased. Thus the Yanan pork has the advantages of tenderness, redness, and fresh and delicious taste, and the economic efficiency of the breeders is prominently increased.

The invention discloses a small molecular polypeptide TAT-siP-PTPN1-HA and application thereof in preparation of a medicament for treating or preventing senile dementia. An artificially synthesized TAT protein transduction structural domain and an siP-PTPN1-HA fusion protein polypeptide TAT-siP-PTPN1-HA are used, TAT can carry the siP-PTPN1-HA protein polypeptide to pass through a blood-brain carrier via blood to be absorbed by neurons, and by applying the small molecular polypeptide TAT-siP-PTPN1-HA to an in-vivo senile dementia model, the biological action of blocking the binding of miR-124 and protein tyrosine phosphatase, non-receptor type 1, PTPN1 3'-non-coding sequence (3'-untranslated region, 3'-UTR) can be effectively realized, the expression of PTPN1 is further effectively reversed, excessive phosphorylation of microtube-associated protein tau and amyloid protein deposition, as well as animal learning and memory disorders represented by an senile dementia model animal are improved, and a new direction is provided for further development of the medicaments for clinical treatment of senile dementia.

The present invention relates to a compound represented by formula (E). The present invention also relates to a compound represented by the formula (E) for use in the treatment or prevention of diseases linked to protein aggregation and/or neurodegenerative diseases. Moreover, the present invention relates to pharmaceutical and diagnostic compositions comprising the compound of the invention as well as to a kit. Furthermore, the present invention relates to a method of imaging deposits of aggregated protein. A kit for preparing a detectably labelled compound of the present invention is also disclosed. Formula (E) wherein X, Y and L are independently nondirectionally selected from —C(R¹)(R²)—, —C(R³)═, —N(R⁴)—, —N═, —N⁺(R⁵)═, —O— and —S—; M and Z are independently nondirectionally selected from formula (I) and formula (II).

The invention discloses a feed capable of improving feed conversion efficiency and protein deposition rate of fresh water fishes and an application of same. The feed includes: white fish meal, casein,soybean oil, fish oil, corn starch, a vitamin premix, choline chloride, a mineral salt premix, CMC (carboxyl methyl cellulose), and cellulose. The feed, even in low content of fish meal, is high in conversion efficiency, thus reducing breeding feed cost. Fresh water fishes fed on the feed in the invention are effectively reduced in fat deposition rate and reduced in liver to body weight ratio, sothat health of liver is improved.

The invention discloses a concentrate feed containing chitosan oligosaccharide for weaning pig, and belongs to the technical field of pig feed. The feed comprises: calcium hydrophosphate, rock flour, table salt, lysine, choline chloride, organic iron, organic selenium, biological chromium, organic manganese, organic zinc, copper sulphate, ferrous sulphate, zinc sulfate, manganese sulfate, 1% of sodium selenite, 2% of calcium iodate, magnesium sulfate, phytase, vitamin A, vitamin D3, vitamin E, vitamin K3, vitamin B1, vitamin B2, vitamin B6, vitamin B12, nicotinamide, calcium pantothenate, chitosan oligosaccharide powder, rice bran meal and bentonite. The chitosan oligosaccharide is added in the feed, and the feed can clear away the oxygen radical in the body, reduce the damage of cells caused by oxidation, protect the intestinal tract of animal, reduce the disease such as diarrhea, reduce the stress of pigs caused by weaning, improve the protein deposition of animal, improve the daily weight gain effectively, and create considerable economic benefit.

A method of perlecan isolation (from the EHS tumor) which produces “clean” (i.e. substantially “pure”) perlecan is disclosed. Clean perlecan is thus produced in sufficient quantities for use in a number of different in vitro and in vivo assays. In addition, this isolation method exploits a newly discovered aggregating property of a ^˜220 kDa heparan sulfate proteoglycan (HSPG) observed during gel filtration chromatography, which allows it to be effectively separated from non-aggregating perlecan. The method employs specific cation exchange, anion exchange, molecular sieve chromatography and immobilized GAG affinity chromatography. It is demonstrated that there are no other contaminating proteins in the perlecan and HSPG preparations, and that the perlecan core protein is intact. Improved, clean perlecan based, rodent models of fibrillar amyloid protein deposition, accumulation and/or persistence in tissues are disclosed.

The invention relates to a premix additive for fattening pigs. The premix additive comprises the following components by weight percent: 6% to 8% of lysine hydrochloride, 3% to 6% of threonine, 0.6% to 2% of DL-methionine, 0.2% to 0.7 of tryptophan, 1.5% to 1.8% of choline chloride, 1% to 1.5% of compound trace elements, 0.6% to 0.8% of vitamin, 0.4% to 0.8% of glycine betaine, 0.2% to 0.7% of magnesium glycinate, 0.2% to 0.4% of sodium selenite, 0.1% to 0.3% of chromium yeast and 79% to 83.1% of carrier. Compared with the prior art, the premix additive provided by the invention has the advantages that the feed quality is improved, the immunity of each pig body is enhanced and the digestive absorption of each pig body is promoted; after the glycine betaine, the magnesium glycinate, the sodium selenite and the chromium yeast are mixed with common raw materials such as corn, bean pulp and bran, the anti-stress ability of each pig body is enhanced, and thus the deposition rate of protein in pork is perfected so as to improve the pork quality; and in addition, the premix additive is sufficient in raw material source, low in cost and good in effect.