Artificial compound feed for improving collagen deposition of schizothorax grahami and application of artificial compound feed
By adding proline, hydroxyproline, eucommia, broad bean and yeast extract to the feed of Kunming schizothorax and optimizing the ratio of feed ingredients, the problem of how to promote collagen deposition in Kunming schizothorax was solved, and the effect of significantly improving the quality of fish meat was achieved.
Patent Information
- Application Number
- CN202511030018.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-07-25
- Publication Date
- 2025-10-03
AI Technical Summary
Existing research has not yet found an effective way to rationally match and utilize feed additives to reduce nutritional antagonism, balance amino acids, and promote collagen deposition in Kunming schizothorax.
A feed formula with appropriate amounts of proline, hydroxyproline, eucommia, broad bean and yeast extract is used, including an animal protein source, a plant protein source, a fat source, a sugar source and a premix, to optimize the ratio of nutrients and prepare a 1.5 mm pellet feed.
Significantly improves collagen deposition in Kunming schizothorax, improves fish meat quality, is low-cost and suitable for feeding.
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Abstract
Description
Technical Field
[0001] The invention relates to the field of fish feed, in particular to an artificial compound feed for improving collagen deposition in Kunming schizothorax. Background Art
[0002] Collagen contains a variety of essential amino acids, and its metabolic byproducts participate in vital life processes, playing a crucial role in cellular nutrient absorption and transport, cell division and proliferation, immunity, and cancer prevention. Collagen is considered the most nutritious ingredient in health foods, supporting improved brain function, maintaining normal endocrine function, and regulating immune function. Consequently, collagen-rich animal foods are often considered a nutritional supplement and hold high market value.
[0003] The Kunming schizothorax, belonging to the Cypriniformes, Cyprinidae, Schizothorinae, and Schizothorax, is a fish endemic to my country's plateau. It is primarily found in the upper reaches of the Yangtze River, the middle and lower reaches of the Jinsha and Yalong Rivers, and the upper reaches of the Wujiang River. Its tender, delicious, nutritious meat, and rich collagen content make it a popular commodity and a key commercial fish in the region.
[0004] Using nutritional manipulation to promote collagen deposition in fish is an effective way to improve fish nutrition and quality. For example, broad beans are widely used in grass carp feeds, significantly increasing the collagen content in grass carp muscle, thereby improving the nutritional value and taste of the fish. The addition of proline, hydroxyproline, vitamin C, glutamine, and α-ketoglutaric acid to feed can promote collagen deposition in the swim bladder of yellow croaker. However, the rational combination and utilization of these feed additives to reduce nutritional antagonism, balance amino acids, and promote collagen deposition in animals, particularly in Kunming schizothorax, remains largely unreported. Summary of the Invention
[0005] The invention aims to provide an artificial feed for Kunming schizothorax, which significantly improves the collagen deposition of Kunming schizothorax and also has a certain promoting effect on its protein deposition.
[0006] In order to achieve the purpose of the present invention, the present invention provides an artificial compound feed for improving collagen deposition in Kunming schizothorax, by adding appropriate amounts of proline, hydroxyproline, eucommia, broad bean, and yeast extract, thereby improving collagen deposition in Kunming schizothorax; the feed is mainly prepared from the following raw materials: animal protein source raw materials, plant protein source raw materials, fat source raw materials, sugar source, premix, and auxiliary materials; wherein the nutritional components of the feed are composed of the following by weight percentage: crude protein 40.825% to 41.407%, crude fat 8.132% to 8.633%, and the remainder being carbohydrates, crude fiber, ash, and moisture.
[0007] The animal protein source raw material is fish meal; the plant protein source raw material is one or more of fermented soybean meal, soybean protein concentrate and corn gluten meal; the fat source raw material is one or more of fish oil, soybean oil and soybean lecithin; the sugar source raw material is one or more of α-starch and cassava starch; the premix is a mixture of eucommia ulmoides, broad beans, yeast extract, proline and hydroxyproline; and the auxiliary materials are a mixture of choline chloride, microcrystalline cellulose, multivitamins, multiminerals, monocalcium phosphate, antioxidants and mildew inhibitors.
[0008] As a preferred embodiment of the present invention, the feed is prepared from the following raw materials in weight percentage: 20-32% fish meal, 21-30% fermented soybean meal, 0-12% soybean protein concentrate, 0-9% corn gluten meal, 1-2% fish oil, 2-3% soybean oil, 1-3% soybean lecithin, 0-14.6% α-starch, 0-13.6% cassava starch, 4-7% microcrystalline cellulose, 1% choline chloride, 1% monocalcium phosphate, 1% multivitamins, 1% mixed minerals, 0.2% antioxidant, 0.2% mildew inhibitor, and 6%-12% premix.
[0009] As a preferred embodiment of the present invention, the feed is prepared from the following raw materials in the following weight percentages: 28% fish meal, 24% fermented soybean meal, 4% soybean protein concentrate, 6% corn gluten meal, 1% fish oil, 2% soybean oil, 2% soybean lecithin, 13.6% tapioca starch, 5% microcrystalline cellulose, 1% choline chloride, 1% calcium dihydrogen phosphate, 1% multivitamins, 1% mixed minerals, trace antioxidants, mildew inhibitors, and 10% premix.
[0010] As a preferred embodiment of the present invention, the feed contains 5% by weight of a premix, which includes 2.5% of Eucommia ulmoides, 4.2% of broad beans, 1% of yeast extract, 1.5% of proline, and 0.8% of hydroxyproline.
[0011] Furthermore, the feed is prepared by the following method: other raw materials and auxiliary materials except fish oil, soybean oil, and soybean lecithin are pre-crushed and passed through a 60-mesh sieve, and accurately weighed according to the formula; first, eucommia ulmoides, broad beans, yeast extract, proline, and hydroxyproline are individually mixed step by step to form a premix; second, after various feed ingredients are mixed step by step, corresponding oils are added respectively and kneaded thoroughly by hand, and then an appropriate amount of water is added and kneaded thoroughly by hand; finally, the mixed feed raw materials are placed in an automatic granulator to prepare feed pellets of about 1.5 mm in size, and the pellets are naturally air-dried to obtain the finished feed.
[0012] The invention also discloses application of the artificial feed for Kunming schizothorax. The artificial feed for Kunming schizothorax is used for feeding Kunming schizothorax and significantly improves collagen deposition of Kunming schizothorax.
[0013] Proline and hydroxyproline are the main amino acids in collagen, accounting for about one-third of the total amino acid content in collagen. They are crucial for the biosynthesis, structure, and strength of collagen. Yeast extract facilitates the absorption and utilization of amino acids by animals.
[0014] The present invention has the advantages of a rational and scientific formula, low cost, and is particularly suitable for feeding Kunming schizothorax. The invention adds appropriate proportions of hydroxyproline, proline, broad bean, eucommia bark, and yeast extract to the formulated feed for Kunming schizothorax, thereby increasing the crude protein content, soluble collagen, insoluble collagen, and total collagen content in the muscle of Kunming schizothorax, thereby improving muscle quality. DETAILED DESCRIPTION
[0015] In order to make the purpose, technical solutions and advantages of the present invention more clear, they are further described below with reference to the embodiments, but the embodiments do not limit the present invention in any form.
[0016] Example 1
[0017] Compound feed group I: An artificial compound feed for improving collagen deposition in Kunming schizothorax, prepared from the following raw materials in percentage by weight: 20% fish meal, 30% fermented soybean meal, 12% soybean protein concentrate, 2% fish oil, 2% soybean oil, 2% soybean lecithin, 6.6% tapioca starch, 8% α-starch, 7% microcrystalline cellulose, 1% choline chloride, 1% calcium dihydrogen phosphate, 1% multivitamins, 1% mixed minerals, 1.5% eucommia ulmoides, 2.7% broad beans, 0.5% yeast extract, 0.8% proline, 0.5% hydroxyproline, 0.2% antioxidant, and 0.2% mildew inhibitor.
[0018] Compound feed group II: An artificial compound feed for improving collagen deposition in Kunming schizothorax, prepared from the following raw materials in weight percentage: fish meal 24%, fermented soybean meal 27%, soybean protein concentrate 8%, corn gluten meal 3%, fish oil 1%, soybean oil 3%, soybean lecithin 1%, tapioca starch 10.6%, α-starch 4%, microcrystalline cellulose 8%, choline chloride 1%, calcium dihydrogen phosphate 1%, multivitamins 1%, mixed minerals 1%, eucommia ulmoides 1.5%, broad beans 2.7%, yeast extract 0.5%, proline 0.8%, hydroxyproline 0.5%, antioxidant 0.2%, and mildew inhibitor 0.2%.
[0019] Compound feed group III: An artificial compound feed for improving collagen deposition in Kunming schizothorax, prepared from the following raw materials in percentage by weight: 28% fish meal, 24% fermented soybean meal, 4% soybean protein concentrate, 6% corn gluten meal, 1% fish oil, 2% soybean oil, 2% soybean lecithin, 13.6% tapioca starch, 9% microcrystalline cellulose, 1% choline chloride, 1% calcium dihydrogen phosphate, 1% multivitamins, 1% mixed minerals, 1.5% eucommia ulmoides, 2.7% broad beans, 0.5% yeast extract, 0.8% proline, 0.5% hydroxyproline, 0.2% antioxidant, and 0.2% mildew inhibitor.
[0020] Compound feed group IV: An artificial compound feed for improving collagen deposition in Kunming schizothorax, comprising the following raw materials in percentage by weight: 32% fish meal, 21% fermented soybean meal, 9% corn gluten meal, 1% fish oil, 2% soybean oil, 1% soybean lecithin, 13.6% α-starch, 10% microcrystalline cellulose, 1% choline chloride, 1% calcium dihydrogen phosphate, 1% multivitamins, 1% mixed minerals, 1.5% eucommia, 2.7% broad bean, 0.5% yeast extract, 0.8% proline, 0.5% hydroxyproline, 0.2% antioxidant, and 0.2% mildew inhibitor.
[0021] Effect detection of feed of the present invention:
[0022] As shown in Table 1, the breeding experiment was conducted in a recirculating aquaculture system in the Aquatic Research Laboratory of the College of Animal Science and Technology of Yunnan Agricultural University. Kunming schizothorax were purchased from the Germplasm Resource Base of the Yunnan Aquatic Technology Extension Station (Kunming, Yunnan). 480 experimental fish (23.29±0.642 g) were randomly divided into 18 breeding tanks (100 cm×50 cm×50 cm, length×width×depth), 40 fish per tank. Four groups of isonitrogenous and isolipid feeds were prepared according to the formula of Example 1 (Table 1), numbered I, II, III, and IV. The measured protein levels in each group of feeds were 40.977%, 40.825%, 41.192%, and 41.407%, respectively; the fat levels were 8.385%, 8.564%, 8.132%, and 8.422%, respectively. Groups of three cages were randomly assigned to the four dietary treatment groups. During the 8-week rearing period, fish were fed twice daily, with feed amounts recorded. Fish growth was recorded by weighing, and their health and environmental changes were observed and recorded. Water quality was regularly tested during the experiment. During the experiment, the water temperature was 26-30.0°C, ammonia nitrogen <0.20 mg / L, nitrite <0.01 mg / L, dissolved oxygen >7.0 mg / L, and pH = 7.4-7.8. Daily feed intake, water temperature, food intake, and health were recorded.
[0023] Table 1 Test feed formula and nutritional components
[0024]
[0025] Note: 1. American white fish meal, imported from the United States, crude protein content 72.37%, crude fat content 11.22%
[0026] 2. Yeast extract was purchased from Hunan Yunbang Biotechnology Co., Ltd., catalog number: YB123624
[0027] 3. Mineral premix, providing per kilogram of feed: NaF, 1 mg; KI, 0.4 mg; CoCl2·6H2O, 25 mg; CuSO4·5H2O, 5 mg; FeSO4·H2O, 40 mg; ZnSO4·H2O, 25 mg; MnSO4·H2O, 30 mg; MgSO4·7H2O, 600 mg; Ca(H2PO4)·2H2O, 1500 mg; NaCl, 50 mg
[0028] 4 Vitamin premix, providing per kilogram of feed: VB1, 25mg; VB2, 45mg; VB6, 20mg; VB12, 0.1mg; VK3, 10mg; VD3, 5mg; inositol, 800mg; pantothenic acid, 60mg; niacin, 200mg; folic acid, 20mg; biotin, 1.2mg; α-tocopherol, 120mg.
[0029] 5. Nutritional composition is measured value.
[0030] After the experiment, all fish in each experimental group were weighed, and their growth and feed utilization were calculated. Four fish were randomly selected from each cage and stored at -20°C for body composition analysis. Six fish were randomly selected from each replicate for blood collection. Blood was drawn from the tail vein, centrifuged, and the supernatant serum was stored at -20°C for serum free hydroxyproline determination. After blood collection, the fish were slaughtered, and muscle was collected and stored at -20°C for collagen analysis.
[0031] Table 2 Effects of different feed formulas on the growth of Schizothorax kunmingensis
[0032]
[0033] Note: Values are expressed as mean ± standard error, n = 3. Values with different lowercase letters within the same row are significantly different (P < 0.05).
[0034] As shown in Table 2, the survival rate (SR) was above 95% among all experimental treatment groups, with no significant differences (P>0.05). Furthermore, there were no significant differences in initial weight (m0) and final fatness (CF) among all experimental treatment groups. Notably, the specific growth rate (SGR) and final weight (m1) of Group III fed Formula Feed were significantly higher than those of Groups I and II fed Formula Feed (P<0.05). Conversely, the feed conversion efficiency (FCR) was significantly lower in Group III fed Formula Feed than in Group IV fed Formula Feed. This indicates that, among all experimental groups, Group III had the most effective growth-promoting effect, improving the growth performance and feed utilization of Kunming schizothorax.
[0035] Table 3 Effects of different feed formulas on the approximate composition of Kunming schizothorax
[0036]
[0037] Note: Values are expressed as mean ± standard error, n = 3. Values with different lowercase letters within the same row are significantly different (P < 0.05).
[0038] The whole-body composition analysis in Table 3 shows that protein deposition in the Kunming schizothoracis in Group III was significantly higher than in the other groups (P < 0.05), and the ash content in Group I was significantly higher than in Group III (P < 0.05). This indicates that Group III had the best protein deposition effect among all experimental groups.
[0039] Table 4 Effects of different feed formulas on Hyp and collagen in Kunming schizothorax tissues
[0040]
[0041] Note: Values are expressed as mean ± standard error, n = 3. Values with different lowercase letters within the same row are significantly different (P < 0.05).
[0042] Collagen analysis in Table 4 indicates that serum free Hyp levels in Kunming schizothorax were not significantly different among all experimental treatment groups (P>0.05). Muscle Hyp and collagen levels in Group III were significantly higher than those in Groups I and II (P<0.05). This suggests that Group III had the best collagen deposition-promoting effect among all experimental groups, increasing collagen content in Kunming schizothorax muscle. Overall, these indicators indicate that Group III improved growth performance, protein deposition, and collagen content in Kunming schizothorax, thereby enhancing the meat quality of the fish.
[0043] The feed formula was further optimized, mainly by making full use of the advantages of compound feed group III, using a constant ratio of plant protein, animal protein, fat, and glycogen, expanding the embodiment, and further optimizing the premix ratio, as shown in Example 2 below.
[0044] Example 2. Compound Feed Group III: An artificial compound feed for improving collagen deposition in Schizothorax kunmingensis, comprising the following raw materials in percentage by weight: 28% fish meal, 24% fermented soybean meal, 4% soy protein concentrate, 6% corn gluten meal, 1% fish oil, 2% soybean oil, 2% soy lecithin, 13.6% tapioca starch, 9% microcrystalline cellulose, 1% choline chloride, 1% calcium dihydrogen phosphate, 1% multivitamins, 1% mixed minerals, 1.5% eucommia ulmoides, 2.7% broad beans, 0.5% yeast extract, 0.8% proline, 0.5% hydroxyproline, 0.2% antioxidant, and 0.2% mildew inhibitor.
[0045] Compound feed group V: An artificial compound feed for improving collagen deposition in Kunming schizothorax, comprising the following raw materials in percentage by weight: 28% fish meal, 24% fermented soybean meal, 4% soybean protein concentrate, 6% corn gluten meal, 1% fish oil, 2% soybean oil, 2% soybean lecithin, 13.6% tapioca starch, 7% microcrystalline cellulose, 1% choline chloride, 1% calcium dihydrogen phosphate, 1% multivitamins, 1% mixed minerals, 2% eucommia ulmoides, 3.5% broad beans, 0.8% yeast extract, 1.2% proline, 0.5% hydroxyproline, 0.2% antioxidant, and 0.2% mildew inhibitor.
[0046] Compound feed group VI: An artificial compound feed for improving collagen deposition in Kunming schizothorax, comprising the following raw materials in percentage by weight: 28% fish meal, 24% fermented soybean meal, 4% soybean protein concentrate, 6% corn gluten meal, 1% fish oil, 2% soybean oil, 2% soybean lecithin, 13.6% tapioca starch, 5% microcrystalline cellulose, 1% choline chloride, 1% calcium dihydrogen phosphate, 1% multivitamins, 1% mixed minerals, 2.5% eucommia ulmoides, 4.2% broad beans, 1% yeast extract, 1.5% proline, 0.8% hydroxyproline, 0.2% antioxidant, and 0.2% mildew inhibitor.
[0047] Compound feed group VII: An artificial compound feed for improving collagen deposition in Kunming schizothorax, comprising the following raw materials in percentage by weight: 28% fish meal, 24% fermented soybean meal, 4% soybean protein concentrate, 6% corn gluten meal, 1% fish oil, 2% soybean oil, 2% soybean lecithin, 13.6% tapioca starch, 3% microcrystalline cellulose, 1% choline chloride, 1% calcium dihydrogen phosphate, 1% multivitamins, 1% mixed minerals, 3% eucommia ulmoides, 5.2% broad beans, 1.5% yeast extract, 1.5% proline, 0.8% hydroxyproline, 0.2% antioxidant, and 0.2% mildew inhibitor.
[0048] Effect detection of feed of the present invention:
[0049] As shown in Table 5, the breeding experiment was conducted in a recirculating aquaculture system in the Aquatic Research Laboratory of the College of Animal Science and Technology of Yunnan Agricultural University. Kunming schizothorax were purchased from the Germplasm Resource Base of the Yunnan Aquatic Technology Extension Station (Kunming, Yunnan). 300 test fish (49.13±0.689 g) were randomly divided into 18 breeding tanks (100 cm×50 cm×50 cm, length×width×depth). Four groups of isonitrogenous and isolipid feeds were prepared according to the formula of Example 2 (Table 5), numbered III, V, VI, and VII. The measured protein levels in each group of feeds were 41.192%, 41.315%, 40.931%, and 41.515%, respectively; the fat levels were 8.132%, 8.427%, 8.633%, and 8.326%, respectively. Three cages were randomly assigned to one of the four dietary treatments. During the 8-week rearing period, fish were fed twice daily, with feed amounts recorded. Fish growth was recorded by weighing, and their health and environmental changes were observed and recorded. Water quality was regularly tested during the experiment. During the experiment, the water temperature was 26-30.0°C, ammonia nitrogen <0.20 mg / L, nitrite <0.01 mg / L, dissolved oxygen >7.0 mg / L, and pH = 7.4-7.8. Daily feed intake, water temperature, food intake, and health were recorded.
[0050] Table 5 Test feed formula and nutritional composition
[0051]
[0052] Note: 1. American white fish meal, imported from the United States, crude protein content 72.37%, crude fat content 11.22%
[0053] 2 Yeast extract was purchased from Hunan Yunbang Biotechnology Co., Ltd., product number: YB123624
[0054] 3. Mineral premix, providing per kilogram of feed: NaF, 1mg; KI, 0.4mg; C o Cl2·6H2O, 25mg; CuSO4·5H2O, 5mg; FeSO4·H2O, 40mg; ZnSO4·H2O, 25mg; MnSO4·H2O, 30mg; MgSO4·7H2O, 600mg; Ca(H2PO4)·2H2O, 1500mg; NaCl, 50mg
[0055] 4 Vitamin premix, providing per kilogram of feed: VB1, 25mg; VB2, 45mg; VB6, 20mg; VB12, 0.1mg; VK3, 10mg; VD3, 5mg; inositol, 800mg; pantothenic acid, 60mg; niacin, 200mg; folic acid, 20mg; biotin, 1.2mg; α-tocopherol, 120mg.
[0056] 5. Nutritional composition is measured value.
[0057] After the experiment, all fish in each experimental group were weighed, and their growth and feed utilization were calculated. Four fish were randomly selected from each cage and stored in a -20°C freezer for body composition analysis. Six fish were randomly selected from each replicate for blood collection. Blood was drawn from the tail vein, centrifuged, and the supernatant serum was stored at -20°C for serum free hydroxyproline determination. After blood collection, the fish were slaughtered, and muscle was randomly selected from three fish, placed in sealed bags, and stored in a -20°C freezer for determination of muscle and swim bladder collagen content. Muscle was collected from the remaining three fish and fixed in formaldehyde for subsequent sectioning.
[0058] Table 6 Effects of different feed formulas on the growth of Schizothorax kunmingensis
[0059]
[0060] Note: The values are expressed as mean ± standard error, n = 3.
[0061] As shown in Table 6, there were no significant differences (P>0.05) in survival rate (SR), initial body weight (m0), final body weight (ml), specific growth rate (SGR), fatness fraction (CF), and feed conversion ratio (FCR) among all experimental treatment groups. This indicates that the changes in the four diet groups (III, V, VI, and VII) had no effect on the growth performance of Kunming schizothorax.
[0062] Table 7 Effects of different feed formulas on the approximate composition of the body of Kunming schizothorax
[0063]
[0064] Note: Values are expressed as mean ± standard error, n = 3. Values with different lowercase letters within the same row are significantly different (P < 0.05).
[0065] The whole body composition analysis in Table 7 shows that the protein content of Kunming schizothoracis in Group VI of Formulated Feed was the highest, significantly higher than that in Group III of Formulated Feed (P < 0.05), indicating that Group VI of Formulated Feed had the best protein deposition effect among all experimental groups.
[0066] Table 8 Effects of different feed formulas on Hyp and collagen in Kunming schizothorax tissues
[0067]
[0068] Note: Values are expressed as mean ± standard error, n = 3. Values with different lowercase letters within the same row are significantly different (P < 0.05).
[0069] Collagen analysis in Table 8 indicates that serum free Hyp levels in Kunming schizothorax were not significantly different among all experimental treatment groups (P>0.05). Muscle Hyp and collagen levels were highest in Group VI, significantly higher than those in Group I (P<0.05). This suggests that Group VI had the best collagen deposition-promoting effect among all experimental groups, increasing collagen content in Kunming schizothorax muscle.
[0070] Based on the above indicators, it can be seen that the formula feed group VI can increase the protein deposition and collagen content of Kunming schizothorax, thereby improving the meat quality of Kunming schizothorax.
[0071] Based on the results of Example 1 and Example 2, it can be seen that this feed formula can improve the protein deposition of Kunming Schizothorax whole fish, and also increase the deposition of collagen in the muscle, thereby improving the meat quality of Kunming Schizothorax.
Claims
1. An artificial feed for improving collagen deposition in Schizothorax kunmingensis, characterized in that: Appropriate amounts of proline, hydroxyproline, eucommia barbata, broad bean, and yeast extract are added to improve collagen deposition in Kunming schizothorax. The feed is mainly prepared from the following raw materials: animal protein source raw materials, plant protein source raw materials, fat source raw materials, sugar source, premix, and auxiliary materials. The nutritional components of the feed are as follows by weight percentage: crude protein 40.825% to 41.407%, crude fat 8.132% to 8.633%, and the remainder is carbohydrates, crude fiber, ash, and moisture.
2. An artificial feed for improving collagen deposition in Schizothorax kunmingensis according to claim 1, characterized in that: The animal protein source raw material is fish meal; the plant protein source raw material is one or more of fermented soybean meal, soybean protein concentrate and corn gluten meal; the fat source raw material is one or more of fish oil, soybean oil and soybean lecithin; the sugar source raw material is one or more of α-starch and cassava starch; the premix is a mixture of eucommia ulmoides, broad beans, yeast extract, proline and hydroxyproline; and the auxiliary materials are a mixture of choline chloride, microcrystalline cellulose, multivitamins, multiminerals, monocalcium phosphate, antioxidants and mildew inhibitors.
3. An artificial feed for improving collagen deposition in Schizothorax kunmingensis according to claim 1, characterized in that: The feed comprises the following raw materials in percentage by weight: 20-32% fish meal, 21-30% fermented soybean meal, 0-12% soybean protein concentrate, 0-9% corn gluten meal, 1-2% fish oil, 2-3% soybean oil, 1-3% soybean lecithin, 0-14.6% α-starch, 0-13.6% cassava starch, 4-7% microcrystalline cellulose, 1% choline chloride, 1% calcium dihydrogen phosphate, 1% complex vitamins, 1% mixed minerals, 0.2% antioxidant, 0.2% mildew inhibitor, and 6%-12% premix.
4. The artificial feed for improving collagen deposition in Schizothorax kunmingensis according to claim 1, characterized in that: The feed is prepared from the following raw materials in the following weight percentages: 28% fish meal, 24% fermented soybean meal, 4% soybean protein concentrate, 6% corn gluten meal, 1% fish oil, 2% soybean oil, 2% soybean lecithin, 13.6% tapioca starch, 5% microcrystalline cellulose, 1% choline chloride, 1% calcium dihydrogen phosphate, 1% multivitamins, 1% mixed minerals, antioxidants, mildew inhibitors, and 10% premix.
5. The artificial feed for improving collagen deposition in Schizothorax kunmingensis according to claim 1, characterized in that: The feed contains 5% by weight of a premix, which comprises 2.5% of eucommia bark, 4.2% of broad bean, 1% of yeast extract, 1.5% of proline and 0.8% of hydroxyproline.
6. An artificial compound feed for improving collagen deposition in Schizothorax kunmingensis according to any one of claims 1 to 5, characterized in that: The feed is prepared by the following method: the raw materials and auxiliary materials other than fish oil, soybean oil and soybean lecithin are pre-crushed and passed through a 60-mesh sieve, and accurately weighed according to the formula; first, eucommia ulmoides, broad beans, yeast extract, proline and hydroxyproline are individually mixed step by step to form a premix; second, after the various feed ingredients are mixed step by step, the corresponding oils are added respectively and kneaded thoroughly by hand, and then an appropriate amount of water is added and kneaded thoroughly by hand; finally, the mixed feed raw materials are placed in an automatic granulator to prepare feed pellets of about 1.5 mm in size, and the pellets are naturally air-dried to obtain the finished feed.
7. The use of the artificial feed for Kunming schizothorax according to any one of claims 1 to 5, characterized in that: The artificial feed for Kunming schizothorax is used for feeding Kunming schizothorax, and significantly improves the collagen deposition of the Kunming schizothorax.