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Nucleic acid detection kit for novel coronavirus COVID-19 and use method thereof

A technology of COVID-19 and detection kits, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, and microorganism measurement/inspection, which can solve the problems of missed diagnosis, low accuracy of detection results, unfavorable epidemic situation, etc., and achieve improvement Accuracy, the effect of shortening the time of diagnosis

Active Publication Date: 2020-05-08
HEMOSMART MEDICAL TECH LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the successful isolation of the new coronavirus COVID-19, the nucleic acid detection of the new coronavirus has become a key link in the diagnosis of cases, and the nucleic acid detection kits for the new coronavirus have appeared on the market, but the kits on the market have the problem of low accuracy of test results , it often happens that a positive case may be tested as a negative result in one test, which is likely to cause missed diagnosis during use, which is not conducive to the prevention and control of the epidemic

Method used

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  • Nucleic acid detection kit for novel coronavirus COVID-19 and use method thereof
  • Nucleic acid detection kit for novel coronavirus COVID-19 and use method thereof
  • Nucleic acid detection kit for novel coronavirus COVID-19 and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] 1. Experimental materials / equipment / reagents:

[0049] 1. Experimental materials:

[0050] Clinical sample RNA extraction kit: Qiagen RNeasy Mini Kit (50) 74104;

[0051] Novel coronavirus COVID-19 nucleic acid detection kit, the main components of the kit are shown in Table 1;

[0052]

[0053] Among them, the 2019 novel coronavirus negative quality control product is obtained by taking an appropriate amount of RNase-Free ddH 2 O is subpackaged into centrifuge tubes under a sterile environment, that is, a negative quality control product is obtained.

[0054] Inspection method: Visual inspection of product appearance, colorless and clear liquid, no sediment, no suspended matter, no floc.

[0055] For the preparation process of the positive quality control product of the novel coronavirus image 3 .

[0056] Production Process:

[0057] (1) Centrifuge 1 μg of dry plasmid powder at 12,000 rpm for 2 min. Add 50 μL ddH 2 O, briefly centrifuge after shaking for 2...

Embodiment 2

[0091] The sequence of the first positive quality control product as shown in SEQ ID NO.10, the second positive quality control product as shown in SEQ ID NO.11, the third positive quality control product as shown in SEQ ID NO.12 Respectively replace the samples in Example 1, detect according to the method of Example 1, each positive quality control product is repeated three times, and the detection results are as follows: Figure 4As shown, where gene E is the third positive quality control product, gene N is the first positive quality control product, and gene ORFlab is the second positive quality control product.

Embodiment 3

[0093] Get 10 negative throat swab samples to detect according to the method of embodiment 1, test result is as follows: Figure 5A with 5B As shown, all 10 samples were negative.

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Abstract

The invention relates to a nucleic acid detection kit for novel coronavirus COVID-19. The kit comprises a first primer pair and a first probe which correspond to Cov-n, and a second primer pair and asecond probe which correspond to Cov-ORF1ab. The kit disclosed by the invention can be used for simultaneously carry out multiple-gene and multiple-site detection on the novel coronavirus, so that thedetection accuracy is improved; and in addition, when the multiple fluorescence PCR reaction is carried out, novel coronavirus detection information can be provided through one-time detection within70 minutes, so that the diagnosis time of the novel coronavirus COVID-19 is shortened. The kit can be used for in vitro qualitatively detecting throat swabs, sputum and bronchoalveolar lavage fluid samples of suspected pneumonia cases infected by the novel coronavirus, suspected aggregated case patients, and other diagnosis people who are required to perform novel coronavirus infection diagnosis or identification.

Description

technical field [0001] The present invention specifically relates to a novel coronavirus COVID-19 nucleic acid detection kit and a method of use thereof. Background technique [0002] Novel coronavirus pneumonia is an acute infectious pneumonia, and its pathogen is a new type of coronavirus not previously found in humans, the novel coronavirus COVID-19. Epidemiological surveys show that the incubation period of the new coronavirus is 1-14 days, and the incubation period is contagious. Asymptomatic infections may also become the source of infection, and the crowd is generally susceptible. In order to control the epidemic, we can only take measures to suspend work, production, and school across the country. If it is possible to quickly detect whether a patient is infected with the new coronavirus COVID-19, it will be of great significance to the control of the epidemic. With the successful isolation of the new coronavirus COVID-19, the nucleic acid detection of the new corona...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/6851C12R1/93
CPCC12Q1/6851C12Q1/701C12Q2600/16C12Q2531/113C12Q2537/143C12Q2563/107C12Q2545/113
Inventor 颜菁杨朝吕晓冬丁文斐
Owner HEMOSMART MEDICAL TECH LTD
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