Novel coronal virus strain and medicinal use thereof

A coronavirus, a new type of technology, applied in antiviral agents, pharmaceutical formulations, viruses/bacteriophages, etc., can solve problems such as lack of support

Inactive Publication Date: 2003-10-22
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This finding is also not supported by other national laboratories

Method used

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  • Novel coronal virus strain and medicinal use thereof
  • Novel coronal virus strain and medicinal use thereof
  • Novel coronal virus strain and medicinal use thereof

Examples

Experimental program
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Embodiment 1

[0024] Throat swab specimens were taken from patients with confirmed SARS, treated with 50 micrograms / ml penicillin, 50 micrograms / ml streptomycin, and 1 microgram / ml amphotericin, and inoculated with Vero E6 cells and HEP-2 cells respectively. 2 Cultivate at 35°C, and observe the cytopathic effect (CPE) every day. After 7 days of culture, the supernatant of the diseased cells is ultracentrifuged with a sucrose gradient, and the virus particles are separated for negative staining observation with an electron microscope. The diameter of the coronavirus-like particles can be seen At 60-100nm, the virus morphology is consistent with the novel coronavirus (SARS-CoV).

Embodiment 2

[0026] Separate the Vero E6 cells infected with the virus and make the cell sheet. When the cell is pathological, take out the infected cell droplet and put it in CO 2Incubate at 35°C to allow the cells to adhere to the wall. Remove the slides, rinse with PBS, remove unattached cells, and air dry. Put it in cold acetone at -20°C for 30 minutes, and dry it in air. Drop 1:10 dilution of convalescent serum from a confirmed SARS patient three weeks later, put it into an incubator at 37°C for 30 minutes, wash with 0.01mol / L pH7.2 PBS three times, and absorb the liquid. Fluorescently labeled goat anti-human IgG antibody, purchased from CHEMICON, was added dropwise, placed in a warm box at 37°C for 30 minutes, washed three times with 0.01mol / L PBS pH7.2, and the liquid was sucked off. After indirect immunofluorescence staining, it can be seen that the edge of the infected cells is stained in a half-moon shape, and the fluorescence is obvious. Normal Vero E6 cells have no fluoresce...

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Abstract

The present invention relates to a new strain of coronavirus and its application. Said invention utilizes the throat-swabbed cell colture of SARS patient to separate out a new type coronavirus, called by name of SARS coronavirus Fudan I strain. By infecting Vero E 6 cell and patient's serum the indirect FA display is positive, after the influenza virus A and B, RS virus and pathogens of pneumococcus, etc. are excluded, the tests show that said separated coronavirus is a variant strain of new coronavirus. Said strain can be used for providing basis for preparing SARS diagnosis reagent and researchnig and producing medicine for resisting and curing SARS disease.

Description

technical field [0001] The invention belongs to the field of microbial viruses, and relates to new strains of novel coronaviruses and their medical applications. Background technique [0002] From December 6, 2002 to February 9, 2003, more than 300 cases of acute respiratory syndrome of unknown cause were found in Guangdong Province of my country, of which 5 cases died. The investigation found that the disease mainly affects the patient's family members and medical staff. The atypical pneumonia epidemic in Guangdong Province of my country was reported to the world on February 11, 2003. It was initially thought that the pathogen might be a newly emerged avian influenza virus. On February 19, 2003, researchers isolated an avian influenza virus (H5N1) from a child case in Hong Kong. The virus is similar to influenza viruses that originated in birds and caused a human outbreak in Hong Kong in 1997, but subsequent studies have ruled out this etiological inference. In late Febr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A61K39/215A61P11/00A61P31/14C12N7/00C12Q1/04G01N33/569
Inventor 姜庆五居丽雯李子华林玉尊俞顺章
Owner FUDAN UNIV
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