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Salt-tolerant pyrene degrading bactericide applied to oil-containing silt

A pyrene-degrading bacteria and salt-tolerant technology, applied in the field of environmental remediation, can solve the problem of difficult biodegradation of pyrene, and achieve the effect of fast degradation and wide adaptability

Inactive Publication Date: 2015-10-07
中海石油环保服务(天津)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a salt-tolerant pyrene-degrading bacterial agent applied in oily mud sand for the problem that pyrene in high-salt oily mud sand is difficult to biodegrade at present

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Insert Bacteria 205 into beef extract-peptone medium (beef extract 5g, peptone 10g, NaCl 5g, water 1000ml, pH7.0 (or 7.1 or 7.2), sterilize at 121°C for 20 minutes), 30°C constant temperature shaker 150r / min for 48 hours. Centrifuge at a high speed of 5000r / min for 10 minutes, discard the supernatant, remove the lower bacterial cell pellet, and wash twice with pH 7.0 (or 7.1, 7.2, 7.3, 7.4 or 7.5) phosphate buffer. The obtained bacterial precipitates were mixed according to the formula in Table 1 to obtain a salt-tolerant pyrene-degrading bacterial agent applied in oily mud sand.

[0027] The composition and ratio of table 1, formula (each component is weight parts)

[0028]

[0029] Recipe 3

Embodiment 2

[0031] Experimental group: Mix 1 g of a salt-tolerant pyrene-degrading bacterial agent used in oily mud sand prepared by formula 1 with 10 g of salty oily mud sand, add 10 ml of nutrient solution (1 g of ammonium chloride, 1 g of dipotassium hydrogen phosphate, 1 g of phosphoric acid Potassium dihydrogen 1g dissolved in 1L distilled water);

[0032] Control group: take 10g of the same saline and oily mud sand as the experimental group, add 10ml of nutrient solution;

[0033] The saline mass concentration of the above-mentioned saline and oily mud sand is 0.5% (through testing, it contains NaCl 0.36%Na 2 SO 4 0.07%); 25 ℃ 60rpm shaker cultured for 20 days, the pyrene content of the experimental group was reduced from 63.2mg / kg to 40.7mg / g, the pyrene degradation rate reached 35.6%, while the pyrene degradation rate of the control group was 8.7%.

Embodiment 3

[0035] Experimental group: Mix 1 g of a salt-tolerant pyrene-degrading bacterial agent used in oily mud sand prepared by Formula 12 with 10 g of salty oily mud sand, and add 10 ml of nutrient solution (1 g of ammonium chloride, 1 g of dipotassium hydrogen phosphate, phosphoric acid Potassium dihydrogen 1g dissolved in 1L distilled water);

[0036] Control group: take 10g of the same saline and oily mud sand as the experimental group, add 10ml of nutrient solution;

[0037] The saline mass concentration of the above-mentioned saline and oily mud sand is 2.6% (through testing, it contains NaCl 1.9%Na 2 SO 4 0.4%); 30 ℃ 100rpm shaker cultured for 30 days, the pyrene content in the experimental group was reduced from 67.8mg / kg to 54.4mg / g, and the pyrene degradation rate reached 19.8%, while the pyrene degradation rate of the control group was 6.9%.

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Abstract

The invention discloses a salt-tolerant pyrene degrading bactericide applied to oil-containing silt. The salt-tolerant pyrene degrading bactericide is characterized by comprising Paracoccus sp. PH205 and a carrier; the Paracoccus sp. PH205 is preserved in the common microorganism center of China Committee for Culture Collection of Microorganisms on May 16th, 2014, and the preservation number is CGMCC No. 9174. Polycyclic aromatic hydrocarbon pyrene in the salt-containing and oil-containing silt can be degraded quickly by the aid of the salt-tolerant pyrene degrading bactericide; As proved by experiments, the salt-tolerant pyrene degrading bactericide has the advantages that the salt-tolerant pyrene degrading bactericide is high in pyrene degrading speed and wide in salinity application range, and can be used for the oil-containing silt with the salinity of 5% at most, and the content of the polycyclic aromatic hydrocarbon pyrene in the oil-containing silt can be reduced effectively.

Description

technical field [0001] The invention belongs to the technical field of environmental restoration, and relates to a salt-tolerant pyrene-degrading bacterial agent applied to oily mud sand. Background technique [0002] Oily sludge mainly comes from the oily sludge discharged during the normal production process of the oil field and the oily sludge formed by falling crude oil and soil caused by various reasons. It is a kind of solid waste with high oil content. Oily mud and sand are mainly produced through four channels: ① ground oil produced during drilling and other construction operations; The rapid development of the petroleum industry has produced a large number of oily mud sands, some typical oily mud sands have an oil content of more than 60%, and the environmental risk is extremely high. In particular, polycyclic aromatic hydrocarbons (Polycyclic Aromatic Hydrocarbons, PAHs) such as pyrene are difficult to degrade naturally, which poses a serious threat to the ecologi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/04C02F11/00
Inventor 王新新石烜陈宇白鹤马蒸钊曹兴涛
Owner 中海石油环保服务(天津)有限公司
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