Feed additive, premix and mixture containing like-bacteriocin and pediococcus acidilactici

A technology of Pediococcus lactis and feed additives, applied in the direction of bacteria, animal feed, animal feed, etc., can solve the problems of not being able to completely get rid of the dependence on antibiotic growth promoters, not being able to fully mobilize probiotics, and unsatisfactory use. Improve the intestinal microbial environment, prevent diarrhea, and eliminate drug residues

Active Publication Date: 2013-03-13
BEIJING DABEINONG TECH GRP CO LTD +1
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AI Technical Summary

Problems solved by technology

[0004] In recent years, the emergence of microecological preparations, enzyme preparations, Chinese herbal medicine preparations and organic acid additives has played a great role in solving this problem, but the use of many products is not ideal, such as: the use of microbial feed additives can play a role in solving this problem. The effect of supplementing or inoculating bacterial strains, but cannot fully

Method used

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  • Feed additive, premix and mixture containing like-bacteriocin and pediococcus acidilactici
  • Feed additive, premix and mixture containing like-bacteriocin and pediococcus acidilactici
  • Feed additive, premix and mixture containing like-bacteriocin and pediococcus acidilactici

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1) Preparation of Bacillus subtilis fermentation broth

[0034] MNB medium was used for fermentation at 30°C for 20 hours, the initial pH was 7, the inoculum size was 3%, and the shaker speed was 225rpm.

[0035] The formula of MNB medium is: glucose, 5.0g / L; peptone, 5.0g / L; meat extract, 3.0g / L; yeast extract powder, 1.0g / L; MgSO 4 7H2O, 0.5g / L; MnSO 4 ·H 2 O, 0.005g / L; pH, 7.0.

[0036] 2) Extraction of bacteriocinoids by ammonium sulfate salting-out method

[0037] Prepare the sterile filtrate from the cultivated Bacillus subtilis bacteria liquid, slowly add the ground ammonium sulfate powder into the sterile filtrate, and stir gently to ensure that the ammonium sulfate is completely dissolved, after stirring slowly for 1 hour, place it in a refrigerator at 4°C . Ammonium sulfate gradient treatment was 70%. After overnight, the solutions with different saturations were taken out and centrifuged separately (10000×g, 30min, 4°C) to collect the precipitate.

[0...

Embodiment 2

[0047] The preparation of embodiment 2 Pediococcus lactis bacteria powder

[0048] 1) Preparation of Pediococcus lactis CGMCC No.6500 seed solution

[0049] Inoculate the strains preserved on the slant of the test tube into an anaerobic tube containing 20mL of MRS liquid medium, and incubate at a constant temperature for 20 hours at 37°C until the number of viable bacteria reaches 10 7 CFU / mL, inoculate at 1% in a 250mL Erlenmeyer flask containing 100mL MRS liquid medium, culture anaerobically at 37°C for 20h, and wait for the number of viable bacteria to reach 107CFU / mL (MRS agar medium plate count) or more, as seeds liquid for use.

[0050] 2) Liquid submerged fermentation culture

[0051] The above-mentioned Pediococcus lactis liquid seed solution was added to a 50L fermenter with an inoculation amount of 1%. The culture parameters were: temperature, 37°C; stirring speed, 50rmp; pH value, 6.0±0.5; culture time, 20h. During the fermentation process, 0.5% of the total ferm...

Embodiment 3

[0059] Preparation of Example 3 Bacteriocins and Pediococcus lactis Mixed Feed Additive

[0060] Prepared by uniformly mixing 50% (wt) of the Bacillus bacteriocin dry powder prepared in Example 1 with 50% (wt) of the Pediococcus lactis powder prepared in Example 2. Its quality standards are: bacteriocinoid dry powder 102mg / g, viable lactic acid bacteria 12 billion / g, moisture ≤ 10%, arsenic ≤ 0.5mg / kg, lead ≤ 1.0mg / kg.

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Abstract

The invention discloses a new strain HT of pediococcus acidilactici and a feed additive containing bacillus like-bacteriocin and pediococcus acidilactici. The collection number of the new strain of pediococcus acidilactici is CGMCC No.6500. The freeze-dried powder of the like-bacteriocin of bacillus subtilis CGMCC No.6511 is sufficiently mixed with the powder of pediococcus acidilactici HT according to a proportion to obtain the feed additive. The feed additive is a green feed additive which improves the feed conversion rate, enhances the productivity and immunity of animals, prevents diarrhea, and realizes an effect of improving the quality of animal products.

Description

technical field [0001] The invention specifically relates to a feed additive containing bacteriocinoids and Pediococcus lactis, a premix and a batch material, and belongs to the field of microbial feed additives. Background technique [0002] Antibiotics not only solve the contradiction between fast growth and low disease resistance, but also bring huge economic benefits to the aquaculture industry, making antibiotics widely used as feed additives. However, the long-term use of antibiotics in feed has brought many negative effects such as drug residues, drug resistance and environmental pollution, which endanger human health. [0003] As food safety issues are getting more and more attention around the world, the call for reducing or stopping the use of antibiotics in animal husbandry is getting higher and higher. Feed additive raw materials that have medicinal properties and do not pollute the environment have become an inevitable trend. [0004] In recent years, the emer...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P21/00A23K1/16A23K1/00C12R1/01
Inventor 胡婷贠桂玲李曼韩科厅
Owner BEIJING DABEINONG TECH GRP CO LTD
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