Nucleic acid detection method for large-scale screening of novel coronavirus infected people and application thereof

Abstract

The invention relates to a nucleic acid detection method for large-scale screening of novel coronavirus infected people and an application thereof. The method comprises the following steps: grouping to-be-detected crowds of novel coronavirus nucleic acid, and carrying out mixed sampling or independent sampling to obtain throat swab specimens; then quantitatively sampling, mixing and centrifuging the specimen, respectively reserving three mixed detection specimens, namely a mixture before centrifugation, a supernatant after centrifugation and a cell precipitate, rapidly extracting all nucleic acids, and finally finishing the on-machine detection and result interpretation of the novel coronavirus nucleic acid. A negative result represents that no novel coronavirus may be infected; accordingto a positive result, nucleic acid screening and verification are carried out in a layered mode, and finally a patient with novel coronavirus nucleic acid being positive is detected. According to theinvention, novel coronavirus nucleic acid detection is carried out under the combination of single collection / mixed collection, mixed detection and concentration, large-scale screening of crowds to bescreened infected by the novel coronavirus can be rapidly and efficiently completed, and the method is especially suitable for general nucleic acid screening of low-risk healthy crowds infected by the novel coronavirus.

Description

technical field [0001] The invention relates to the field of detection of virus infection, especially a nucleic acid detection method for rapid screening of whether a large-scale low-risk population is infected with a novel coronavirus. Background technique [0002] After the outbreak of novel coronavirus infection in some areas, it is necessary not only to isolate close contacts, sub-close contacts, and residential quarters, key places, and high-risk groups where the confirmed cases are located, and to conduct nucleic acid tests for new coronaviruses one by one, but also to fully lift the quarantine. Before returning to work and school, it is often necessary to conduct a thorough investigation of the new coronavirus nucleic acid on the entire region or urban population related to the outbreak. It is a very good way to carry out large-scale and rapid nucleic acid screening of whether low-risk and healthy people around the epidemic area are infected with the new coronavirus. ...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to realize large-scale and rapid nucleic acid screening of whether the low-risk and healthy people around the epidemic area are infected with the new coronavirus after the outbreak of the new coronavirus infection in a local area, which is helpful for the prevention and control of the epidemic during the epidemic. Solve the "bottleneck" problem that restricts the daily throughput of new coronavirus nucleic acid detection in large-scale populations

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