Nucleic acid detection method for large-scale screening of novel coronavirus infected people and application thereof
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A coronavirus, detection method technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, and the determination/inspection of microorganisms, which can solve problems such as bottlenecks
Pending Publication Date: 2020-10-30
THE FIFTH MEDICAL CENT OF CHINESE PLA GENERAL HOSPITAL
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[0006] The purpose of the present invention is to realize large-scale and rapid nucleic acid screening of whether the low-risk and healthy people around the epidemic area are infected with the new coronavirus after the outbreak of the new coronavirus infection in a local area, which is helpful for the prevention and control of the epidemic during the epidemic. Solve the "bottleneck" problem that restricts the daily throughput of new coronavirus nucleic acid detection in large-scale populations
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no. 1 example
[0016] 120 persons waiting for inspection from low-risk areas after the outbreak of the new coronavirus infection without fever and other related symptoms, first took one tube per person, took oropharyngeal swabs, and obtained individual sampling tube specimens.
[0017] Then, divide 120 individual sampling tube specimens into 5 groups according to a group of 24 tubes, namely A, B, C, D, and E. Take 200ul specimens from each tube in each group, put them in a 5ml centrifuge tube, and mix them evenly. , take 200ul specimens from each group, which is mixed test No. 1 specimen, which are A1, B1, C1, D1 and E1 respectively. The rest of the mixture is centrifuged at 3000 rpm, centrifuged for 10 minutes, and 200 μl of the supernatant after centrifugation is collected. Mixed test No. 2 specimens, respectively A2, B2, C2, D2 and E2, before the remaining supernatant is completely removed, reserve 200 μl for diluting the mixed cell sediment in the centrifuge tube after centrifugation, mix...
no. 2 example
[0020] 120 persons to be tested from low-risk areas after the outbreak of the new coronavirus infection without fever and other related symptoms, firstly divided into groups of 3, took oropharyngeal swabs, completed 3:1 mixed sampling and individual sampling, and obtained 40 Mixed sampling tube specimens and 120 individual sampling tube specimens.
[0021]Then, divide the 40 mixed sampling tube specimens into 4 groups according to a group of 10 tubes, namely A, B, C, and D. Take 200ul specimens from each tube in each group and place them in a 5ml centrifuge tube. After mixing evenly, each Take 200ul specimens from each group, which is mixed test No. 1 specimen, which are A1, B1, C1 and D1 respectively. The remaining mixture is centrifuged at 3000 rpm for 10 minutes, and 200 μl of supernatant after centrifugation is collected, which is mixed test No. 2. The samples were A2, B2, C2 and D2. Before the remaining supernatant was completely removed, 200 μl was reserved for diluting ...
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Abstract
The invention relates to a nucleic acid detection method for large-scale screening of novel coronavirus infected people and an application thereof. The method comprises the following steps: grouping to-be-detected crowds of novel coronavirus nucleic acid, and carrying out mixed sampling or independent sampling to obtain throat swab specimens; then quantitatively sampling, mixing and centrifuging the specimen, respectively reserving three mixed detection specimens, namely a mixture before centrifugation, a supernatant after centrifugation and a cell precipitate, rapidly extracting all nucleic acids, and finally finishing the on-machine detection and result interpretation of the novel coronavirus nucleic acid. A negative result represents that no novel coronavirus may be infected; accordingto a positive result, nucleic acid screening and verification are carried out in a layered mode, and finally a patient with novel coronavirus nucleic acid being positive is detected. According to theinvention, novel coronavirus nucleic acid detection is carried out under the combination of single collection / mixed collection, mixed detection and concentration, large-scale screening of crowds to bescreened infected by the novel coronavirus can be rapidly and efficiently completed, and the method is especially suitable for general nucleic acid screening of low-risk healthy crowds infected by the novel coronavirus.
Description
technical field [0001] The invention relates to the field of detection of virus infection, especially a nucleic acid detection method for rapid screening of whether a large-scale low-risk population is infected with a novel coronavirus. Background technique [0002] After the outbreak of novel coronavirus infection in some areas, it is necessary not only to isolate close contacts, sub-close contacts, and residential quarters, key places, and high-risk groups where the confirmed cases are located, and to conduct nucleic acid tests for new coronaviruses one by one, but also to fully lift the quarantine. Before returning to work and school, it is often necessary to conduct a thorough investigation of the new coronavirus nucleic acid on the entire region or urban population related to the outbreak. It is a very good way to carry out large-scale and rapid nucleic acid screening of whether low-risk and healthy people around the epidemic area are infected with the new coronavirus. ...
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