Crude biological derivatives competent for nucleic acid detection

a nucleic acid detection and nucleic acid technology, applied in the field of biological unit lysates or admixtures of body fluids for rna analysis, can solve the problems of genomic dna loss and the inability to use genomic dna as an internal standard, and achieve the effects of reducing the number of cycles
US20050277121A1Inactive Publication Date: 2005-12-15APPL BIOSYSTEMS INC
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Patent Information

Authority / Receiving Office
US · United States
Current Assignee / Owner
APPL BIOSYSTEMS INC
Publication Date
2005-12-15
Estimated Expiration
Not applicable · inactive patent
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Abstract

The invention relates generally to the fields of making biological unit lysates or admixtures of body fluids and of RNA analysis. More specifically, it relates to direct methods for the detection of a specific sequence of RNA in a biological unit, for example a virus, cell or tissue sample, or a body fluid, for example saliva, sputum, blood plasma, etc. More generally, the invention may be used to enzymatically manipulate and protect the RNA in lysate or bodily fluids for a number of applications.
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Description

[0001] The government may own rights in the present invention pursuant to grant number R44 HL69718 from National Institutes of Health National Heart, Lung, and Blood Institute.BACKGROUND OF THE INVENTION

[0002] 1. Field of the Invention

[0003] The present invention relates generally to the fields of making biological unit lysates or admixtures of body fluids for RNA analysis. More specifically, it teaches a more direct method for the detection of a specific sequence of RNA in a biological unit, for example a virus, cell or tissue sample, or a body fluid, for example saliva, sputum, blood plasma, etc. More generally, the invention may be used to enzymatically manipulate and protect the RNA in lysate or bodily fluids for a number of applications.

[0004] 2. Description of Related Art

[0005] There are many molecular biology techniques that can be used to analyze RNA or RNA-containing samples. For example, reverse transcription followed by the polymerase chain reaction (RT-PCR) is one of...

Claims

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