Nucleic acid detection cassette based on magnetic bead transfer and valve controlled pipetting and detection method thereof

Abstract

The invention discloses a nucleic acid detection cassette based on magnetic bead transfer and valve controlled pipetting and a detection method thereof, a cassette body is divided into a nucleic acidextraction area and an amplification detection area, the amplification detection area is provided with a rotary valve tube, a PCR tube, a gas pump interface, a gas channel and a liquid channel, the gas channel respectively extends from the rotary valve tube to a liquid mixing tube and the gas pump interface. The liquid channel respectively extends from the rotary valve tube to a elution tube, theliquid mixing tube and the PCR tube; and a rotary valve for controlling the opening and closing of the gas channel and the liquid channel is arranged in the rotary valve tube. According to the presentinvention, the clinical collection throat swab without any treatment can be directly added, the nucleic acid extraction is achieved by externally driving a magnetic rod and a magnetic rod sleeve, theopening and the closing of the related liquid channel and the gas channel are achieved by controlling the rotary valve, the directional transfer of the liquid can be achieved under the effect of theexternal pump, and finally the real-time fluorescence PCR amplification detection is performed in the PCR tube, so that sample input and result output can be simply and efficiently realized.

Description

technical field [0001] The invention relates to a detection device and a detection method, in particular to a nucleic acid detection cartridge and a detection method based on magnetic bead transfer and valve-controlled pipetting. Background technique [0002] Thanks to the advantages of high sensitivity and high specificity of nucleic acid detection, it is more and more widely used in various fields of biomedicine, especially in the field of infectious disease detection. In the prevention and control of infectious diseases, timely and accurate diagnosis of suspected cases can win valuable time for the prevention and control of the epidemic. However, nucleic acid detection requires cumbersome nucleic acid extraction and time-consuming PCR amplification detection steps, which require professional experimenters to complete on different experimental equipment. The whole process takes several hours, which not only greatly reduces the efficiency of nucleic acid detection, but also...

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Problems solved by technology

However, nucleic acid detection requires cumbersome nucleic acid extraction and time-consuming PCR amplification detection steps, which require professional experimenters to complete on different experimental equipment. The whole process takes several hours, which not only greatly reduces the efficiency of nucleic acid detection, but also due to The transfer of samples between different devices during the process may also cause cross-contamination, which will affect the test results

[0003] Based on the above problems, some cartridge-type integrated nucleic acid detection systems have been launched on the market, which can integrate nucleic acid extraction and amplification detection in a cartridge or microfluidic chip. However, most of the cartridges currently use driver The working principle of the liquid, the cartridge structure is complicated, the cost is expensive, and the work efficiency is not high. For some special samples such as throat swab samples, it requires cumbersome steps such as sample inactivation and pathogen release in the early stage, and it is impossible to directly add samples for detection. The field of infectious disease detection has certain limitations

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