The invention discloses a virus nucleic acid extraction kit based on nano magnetic beads. The lysis liquid comprises guanidine thiocyanate/guanidine hydrochloride, a nonionic surfactant, tris (hydroxymethyl) aminomethane hydrochloride and ethylenediamine tetraacetic acid, and the nonionic surfactant comprises one or more of triton X-100, Tween 20 and ethyl phenyl polyethylene glycol. The binding liquid is isopropanol, the volume ratio of isopropanol to pyrolysis is 1: 1, and the magnetic beads are silicon oxide hydroxyl nano magnetic beads. According to the kit, a nano magnetic bead extractionmethod is adopted, isopropanol is used as a binding liquid, making sample splitting, binding of nucleic acid and magnetic beads to be done in one step. The steps are simple, convenient and easy to implement, and the automatic extraction process of virus nucleic acid can be completed quickly at high flux within 9 min. The kit disclosed by the invention can be used for extracting RNA and DNA of viruses in sample, such as plasma, serum, nasopharynx swabs, urine, secretion liquid, virus concentrate, cell culture liquid supernatant and virus preservation liquid.