The invention belongs to the field of
cell measurement, and discloses a high-precision rod-shaped bacterial
cell length measurement method, which comprises the following steps: S101, segmenting an image containing bacterial cells to be measured to obtain a
mask image and a flow field image; s102, separating a
mask and a flow field of each bacterial
cell in the
mask image and the flow field image to obtain a mask of a single bacterial cell and a flow field of a single bacterial cell; s103, respectively acquiring a smooth curve skeleton of each bacterial
cell based on the mask and the flow field; s104, extending the smooth curve skeleton to the edge of the cell to obtain a
cytoskeleton; and S105, calculating the length of the
cytoskeleton. The high-precision rod-shaped bacterial cell
length measurement method provides a high-precision, rapid and automatic solution for rod-shaped bacterial cell
length measurement.