6 results about "Equine herpesvirus" patented technology

The invention relates to a loop-mediated isothermal amplification method for rapid inspection and quarantine of horse herpes viruses 1 and 4 of imported racing horses. The loop-mediated isothermal amplification method comprises the following steps: S1, obtaining a biological sample of the imported racing horses; s2, extracting a nucleic acid template from the biological sample; s3, mixing the nucleic acid template with a reaction system containing nucleic acid polymerase and a specific loop-mediated isothermal amplification primer group aiming at the equine herpes virus type 1 or type 4; s4, performing a nucleic acid amplification reaction under a constant temperature condition; and S5, detecting an amplification product, and judging whether the imported racing horse is infected with the horse herpes virus type 1 or type 4 or not. The loop-mediated isothermal amplification method for rapid inspection and quarantine of the imported racing horse herpes viruses type 1 and type 4 can effectively meet the requirements of rapid customs clearance and epidemic disease prevention and control of imported racing horses.

The application discloses a use of hyperoside in resisting equid herpesvirus 8 (EHV-8) infection. Through qPCR, Western Blot and other experimental techniques, it is revealed on multiple levels that the hyperoside can significantly inhibit the replication of EHV-8, and the mechanism of the hyperoside in resisting the EHV-8 infection is clarified from the aspect of blocking the internalization of the EHV-8, so that the hyperoside can be applied in preparing an anti-EHV-8 medicine.

The present invention relates i.a. to an EHV (Equine Herpesvirus) comprising a Feline Herpes Virus (FHV) Antigen encoding sequence inserted into ORF70 (US4) and / or ORF1 / 3. Furthermore, the present invention relates to methods for immunizing a feline comprising administering to such feline an immunogenic composition of the present invention. Moreover, the present invention relates to methods for the treatment or prophylaxis of clinical signs caused by Feline Herpes Virus in a feline.

The invention relates to a method for detecting equine herpes viruses type 1 and type 4 through visual loop-mediated isothermal amplification based on color change. The method comprises the following steps: S1, extracting nucleic acid of a sample to be detected; s2, preparing a reaction system containing an LAMP primer group designed for horse herpes virus, DNA polymerase and a color indicator; s3, performing amplification reaction on the reaction system under a constant-temperature condition; and S4, judging a detection result by observing the color change of the reaction system. The method aims at overcoming the defects that in the prior art, an horse herpes virus detection method is tedious in operation and long in consumed time, or professional equipment is needed and the like, and particularly provides a more efficient and convenient horse herpes virus detection means aiming at the requirements of on-site rapid detection and basic application.

The present invention is directed to an immunogenic composition comprising an antigen of Equine Herpes Virus type 1 (EHV-1) and an antigen of Equine Herpes Virus type 4 (EHV-4). The immunogenic composition reduces viremia and other clinical reactions in horses infected with EHV-1 when compared to non-treated horses but also when compared to EHV-1 vaccinated horses.

The invention relates to the field of molecular biology diagnosis, in particular to a loop-mediated isothermal amplification method and kit for detecting equine herpes viruses 1 and 4, and the method comprises the following steps: S1, designing and constructing loop-mediated isothermal amplification (LAMP) primer groups aiming at gB gene specific sequences of equine herpes viruses 1 and 4, each primer group comprising six primers F3, B3, FIP, BIP, LoopF and LoopB; s2, preparing an LAMP (loop-mediated isothermal amplification) reaction system, wherein the reaction system comprises the primer group, a buffer solution, dNTPs (deoxyribonucleoside triphosphates), divalent metal ions and DNA (deoxyribonucleic acid) polymerase; s3, adding nucleic acid of a sample to be detected into the reaction system; s4, carrying out LAMP reaction under the condition of constant temperature; and S5, detecting an LAMP reaction amplification product. The loop-mediated isothermal amplification method for detecting the equine herpes viruses 1 and 4 and the kit thereof can be used for efficiently and accurately detecting the equine herpes viruses 1 and 4, so that the limitations of the traditional detection method in the aspects of time, operation complexity and sensitivity are overcome; and a powerful technical means is provided for rapid diagnosis and prevention and control of horse herpes virus diseases.