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Application of arbidol hydrochloride in preparation of anti-enterovirus medicines

A technology of arbidol hydrochloride and enterovirus, which is applied in antiviral agents, pharmaceutical formulas, medical preparations containing active ingredients, etc.

Inactive Publication Date: 2012-05-09
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Yet so far, also do not apply Arbidol hydrochloride to the prevention and treatment of hand, foot and mouth disease

Method used

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  • Application of arbidol hydrochloride in preparation of anti-enterovirus medicines
  • Application of arbidol hydrochloride in preparation of anti-enterovirus medicines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The toxic effect of embodiment 1 Arbidol hydrochloride on RD cells

[0015] (1) According to Mosmann, the MTT method was established to detect the cytotoxicity of drugs.

[0016] Disperse the well-grown cells into a single cell suspension with trypsin containing 0.02% EDTA, and then adjust the cells to 1×10 with cell culture medium containing 10% calf serum 5 / ml concentration in 96-well plate, 0.2ml per well. Set at 37°C, 5% CO 2 Cultivate in the incubator for 24h. After the cells grow into a single layer, discard the supernatant of the culture medium, and replace with a maintenance solution containing different concentrations of drugs, 200 μl per well. The final concentrations of the drugs were 62.50, 31.25, 15.63, 7.81, 3.91 μg / ml, respectively. Four wells were repeated for each concentration, and a normal cell control group was set up for the experiment. After continuing to culture for 48 h, the cell viability was detected by MTT assay. Results: The toxic ef...

Embodiment 2

[0017] Example 2 Pharmacodynamic study of Arbidol hydrochloride against enterovirus type 71

[0018] 1. The direct killing effect of Arbidol hydrochloride on enterovirus 71

[0019] 50TCID 50 EV71 was mixed with different concentrations of Arbidol hydrochloride solution, the final concentrations were 8, 4, 2, 1, 0.5 μg / ml, respectively, added to the RD cells that had grown into a monolayer at 4 °C for 6 hours, and after 2 hours of adsorption , discard the supernatant, add cell maintenance solution, 37 ° C, 5% CO 2 Continue to grow in the incubator. CPE was observed daily, and when the virus control lesions reached +++~++++, the virus inhibition rate was detected by MTT method. The therapeutic index was used as an evaluation index to measure the inhibitory effect of the drug on the virus. A virus control and a cell control were also set up. Taking TI as the evaluation index of the drug's inhibitory effect on EV71, TI=TC 50 / IC 50 , the larger the TI value, the stronge...

Embodiment 3

[0024] Example 3: Experimental research on anti-EV71 of Arbidol in vivo

[0025] 40 female BALB / c mice (7-9 g) were randomly divided into 4 groups: ① normal animal control group, fed with normal saline; ② virus infection model group, fed with normal saline after virus infection; ③ high-dose treatment group (0.1g·kg -1 d -1 ); ④ low dose treatment group (0.05g·kg -1 d -1 ).

[0026] Mice were anesthetized with ether and treated with 15LD 50The virus liquid was orally infected, each 0.05 mL, and the normal animal control group was orally infected with normal saline instead of the virus liquid. Two hours after infection, intragastric administration was started, administered twice a day, 0.1 mL each time, and administered continuously for 5 days. Observe for 15 days. The mice were fasted for 8 hours before being sacrificed, and the animals were sacrificed by removing the eyeballs, bloodletting, and dismounting the cervical vertebrae. The intestinal tissue was taken out an...

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Abstract

The invention discloses a new application of arbidol hydrochloride in preparation of anti-enterovirus medicines. The research disclosed in the invention indicates that the arbidol hydrochloride has the function of resisting hand-foot-mouth disease virus (enterovirus 71) in cells and animal bodies, and has effective antiviral actions in the dosage mode before and after the virus infection. When the compound is used as an antiviral drug for directly killing virus and interdicting and preventing virus infected cells, the treatment indexes (TI) are respectively 3.08 (directly killing virus), 23.55 (interdicting virus invasion) and 2.5 (inhibiting multiplication of virus). The invention provides a new effective way for treating hand-foot-mouth disease virus infection, and has practical application value.

Description

technical field [0001] The invention relates to an antiviral drug, specifically the application of arbidol hydrochloride in the preparation of a drug for treating hand, foot and mouth disease. Background technique [0002] Hand-foot-mouth disease (HFMD) is a common infectious disease caused by a variety of enteroviruses, among which enterovirus type 71 (EV71) and coxsackievirus type 16 (CoxAl6) are the most common. Toddlers and children are mainly affected. Most patients have mild symptoms, with fever and rashes or herpes on the hands, feet, mouth, buttocks and other parts as the main features. A small number of patients may develop encephalitis, meningitis, myelitis, peripheral neuritis, acute flaccid paralysis, myocarditis, etc., and individual severe cases progress rapidly and may die due to neurogenic pulmonary edema and cardiopulmonary failure. During the epidemic period of hand, foot and mouth disease, kindergartens, nurseries, schools may be collectively infected an...

Claims

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Application Information

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IPC IPC(8): A61K31/4045A61P31/14
Inventor 杨占秋王雨刘强凌佳馨熊海蓉刘媛媛罗凡侯炜肖红
Owner WUHAN UNIV
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