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Harmful mycogone perniciosa and ergot sterane type triterpenoid prepared by fermentation as well as method

A technology of ergostane-type and verrucous mold, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc.

Inactive Publication Date: 2015-06-03
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no report on the preparation of ergostane-type triterpenes by the fungus Verrucous verrucosus

Method used

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  • Harmful mycogone perniciosa and ergot sterane type triterpenoid prepared by fermentation as well as method
  • Harmful mycogone perniciosa and ergot sterane type triterpenoid prepared by fermentation as well as method
  • Harmful mycogone perniciosa and ergot sterane type triterpenoid prepared by fermentation as well as method

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0023] Embodiment 1, the isolation and identification of bacterial strain harmful verruca

[0024] 1. Isolation of strains

[0025] Strain 88362 was isolated from the basidiocarp of Inocybe maculata Boud collected from the broad-leaved forest in Changbai Mountain, Jilin Province in August 2008. Put the basidiocarp into a sterile plastic bag, bring it back to the laboratory from the forest land, put it on the test bench to dry at room temperature for 2 hours, make the surface dry, and tear it off with sterilized tweezers under the alcohol lamp on the ultra-clean workbench For the epidermis of the cap, use a sterile scalpel to cut a little bacterial flesh and place it on a PDA slope for cultivation. After colonies grow, use an inoculation needle to cut small pieces of hyphae from the edge of the colony, transfer to another PDA slope, purify and store.

[0026] 2. Identification of strains

[0027] 1. Morphological identification

[0028] Strain 88362 grows faster on PDA mediu...

Embodiment 2

[0035] A method for preparing ergostane-type triterpenes by fermenting Verrucous verrucosus mold, comprising the steps of:

[0036] (1) Fermentation culture

[0037] ① Strain activation: Mycelium of Mycogone perniciosa 88362 was inoculated on a PDA plate and cultured at 25°C for 5 days. PDA plate: 200 grams of potato, 20 grams of glucose, 15 grams of agar plus distilled water to 1L, sterilized by high pressure steam at 121 ° C for 30 minutes to make a plate;

[0038] ②Seed cultivation:

[0039] After the bacteria overgrow the PDA plate, put five 1cm 2 Inoculate the 250mL Erlenmeyer flask containing 50mL seed culture medium with the bacteria block of the same size and cultivate it on a shaker at 25°C for 5 days (rotation speed: 200rpm) to obtain a seed culture solution. The seed culture medium per liter contains: Glucose 4 grams, 10 grams of malt extract, 4 grams of yeast extract, the balance is water, pH 6.0; add 50 mL of medium to each 250 mL Erlenmeyer flask, and steriliz...

Embodiment 3

[0048] A method for preparing ergostane-type triterpenes by fermenting Verrucous verrucosus mold, comprising the steps of:

[0049] (1) Insert Mycogone perniciosa 88362 into the seed culture medium, and cultivate it at 20°C for 3 days to obtain the seed culture solution, and insert the seed culture solution into the solid medium at a volume ratio of 1:30 , cultured statically at 20°C for 60 days to obtain a fermentation culture; the seed culture medium contained per liter: 4 grams of glucose, 10 grams of malt extract, 4 grams of yeast extract, and the balance was water, with a pH value of 6.0; the solid culture The base consists of 160 grams of rice and 240 mL of distilled water;

[0050] (2) adding ethyl acetate to the fermentation culture for extraction to obtain an extract, and distilling the extract under reduced pressure to obtain a crude extract;

[0051] (3) The crude extract is subjected to silica gel column chromatography (200-300 mesh column chromatography silica ge...

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Abstract

The invention discloses harmful mycogone perniciosa 88362 with the preservation number of CGMCC NO.8352. Ergot sterane type triterpenoid prepared by fermenting the harmful mycogone perniciosa has a structural formula shown in the specification. The invention provides a new strain, two new components and a method for preparing the new compounds by utilizing the new strain, and a material base is provided for developing and utilizing natural active substances from microorganisms.

Description

technical field [0001] The invention relates to a harmful Verrucospora fungus, an ergostane-type triterpene prepared by using the fungus and a preparation method thereof. Background technique [0002] Triterpenoids are a very important class of natural products, and a large number of literatures report their chemical structure, biological activity, biosynthesis and chemical synthesis. Its structure is varied, and some of them have significant antibacterial, antitumor, anti-HIV and other activities and the effect of improving immunity, showing potential drug development value, and have attracted the attention of organic chemists and biochemists. [0003] Harmful verrucous mold is the pathogen of brown rot of dung-grass saprophytic edible fungi such as mushrooms, straw mushrooms, and Pteris cerevisiae. Its pathogenesis may be that some metabolites secreted by it act on the mushroom body and cause brown rot. [0004] At present, there is no report on the preparation of ergost...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P33/00C07J9/00C12R1/645
Inventor 贺伟刘东泽姜文侠刘琦杨萍
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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