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Novel Antrodia cinnamomea isolate and culture product thereof

A technology for isolates, Antrodia cintrodia, applied in the field of novel isolates of Antrodia cinnamomea and its culture products

Inactive Publication Date: 2015-12-09
GUAN WUN AGRI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Antrodia cinnamomea strains are the core focus of producing Antrodia cinnamomea products, but the efficacy and activity of different strains vary greatly, so it is extremely important to screen and obtain excellent Antrodia cinnamomea strains

Method used

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  • Novel Antrodia cinnamomea isolate and culture product thereof
  • Novel Antrodia cinnamomea isolate and culture product thereof
  • Novel Antrodia cinnamomea isolate and culture product thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment one, the solid fermentation product cultivation of Antrodia camphorata bacterial strain

[0034] Antrodia camphorata strains BCRC35398 and BCRC36711 were purchased from the Institute of Food Industry Development. Antrodia camphorata strains GW01, GW02, GW03, GW04 and GW05 are the strains of Antrodia camphorata isolated by the applicant.

[0035] The above 7 strains were taken out from the glycerol tubes of the -80°C freezer and activated, and then inoculated on potato dextrose agar (PDA) (Deveco, USA) and malt extract agar (MEB) (Deveco, USA) plates. After culturing at 25°C for 14-21 days, add sterile water, break it with a homogenizer, inoculate it in a pre-sterilized Erlenmeyer shaker flask containing 50 grams of grain matrix, cultivate it at 25°C for 30 days, and inoculate it with 50 The solid fermented product containing mycelia can be obtained by drying at ℃.

Embodiment 2

[0036] Embodiment two, the analysis of DMB, SY-1 and MBDD content in Antrodia camphorata solid fermented product

[0037] (1) Analytical method

[0038] Weigh 1 gram of Antrodia camphorata solid fermentation product into a centrifuge tube, add 5ml of methanol into the centrifuge tube, shake it in an ultrasonic oscillator for 15 minutes, draw up the methanol solution with an injection syringe, and filter it with a 0.45μm filter membrane group, then place the filtrate in In a dedicated tube for liquid chromatography. The contents of three active metabolites of Antrodia camphorata, including DMB, SY-1 and MBDD, were analyzed by HPLC.

[0039] HPLC analysis conditions are as follows:

[0040] Chromatography column: MN, UNCLEODURC18FTEC, 5μm,

[0041] Inner diameter 4.6mm×25em

[0042] Fluorescence detector: PDA (photodiodearray, photodiode array) UV180 ~ 300nm

[0043] Mobile phase solution: acetonitrile and water at a ratio of 90:10 (v / v) at the beginning of the analysis, th...

Embodiment 3

[0058] Embodiment three, the morphology and molecular identification of bacterial strain GW03

[0059] (1) Morphological observation:

[0060] Strain GW03 was inoculated on PDA (American Difco) and MEA (American Difco) medium, cultured at 25°C for 35 days, all of which would produce peach fruit rot aroma; the average colony diameter on the MEA plate for 35 days was about 55-60mm, The colony is white, light orange to light orange from the inside to the outside, the aerial hyphae are fluffy, powdery, cotton wool to wooly, and the back of the colony is light orange; the average diameter of the colony on the PDA plate for 35 days is about 45 -50mm, the colony is light yellow, orange to light orange from the inside to the outside, the aerial hyphae are fluffy, powdery, cotton wool to wooly, and the back of the colony is light yellow.

[0061] The strain GW03 was observed under an optical microscope, and a large number of conidia could be observed. The conidia are oval, rod-shaped...

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Abstract

The invention relates to a Antrodia cinnamomea isolate and a method for preparing an Antrodia cinnamomea culture product. The invention also provides a method for preparing 2,3-dimethoxy-5-methyl-p-benzoquinone (DMB), 4,7-dimethoxy-5-methyl-1,3-benzodioxole (SY-1) and / or 5-methyl-benzo[1,3]dioxole-4,7-diol (MBDD).

Description

technical field [0001] The present invention relates to a novel isolate of Antrodiacinnamomea, culturing said isolate to obtain a compound containing a large amount of 2,3-dimethoxy-5-methyl-p-benzoquinone (DMB), 4,7-di Methoxy-5-methyl-1,3-benzodioxole (SY-1) and 5-methyl-benzo[1,3]dioxole-4, Methods of culture products of 7-diol (MBDD), and compositions comprising said culture products. The present invention also relates to a method for preparing DMB, SY-1 and / or MBDD from said culture product. Background technique [0002] Antrodiacinnamomea (Zhang T.T. (Chang, T.T.) et al., 1995. Mycological Research (Mycological Research) 99: 756-758) is a Taiwan endemic fungus that only grows in Cinnamomum camphora, because it contains adenosine (adenosine), polysaccharides (polysaccharides) (Chen J.J. (Chen, J.J.) et al., 2007. Journal of Natural Products (Journal of Natural Products) 70:989-992), triterpenoids (triterpenoids) (Chen C.H. (Chen, C.H.) et al., 1995. Natural Product J...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A61K36/07A23L1/28C12R1/645
Inventor 林欣榜张家韵黄祥凤谢松源吴明德陈彦霖
Owner GUAN WUN AGRI BIOTECH
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