Method for preparing culture medium for Antrodia cinnamomea liquid fermentation

A technology of liquid fermentation and production method, applied in application, fertilizer mixture, fertilization device, etc., can solve the problems of high price and inability to meet large-scale industrial production

Inactive Publication Date: 2014-11-12
JINYES QINGDAO BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the Antrodia camphorata liquid fermentation medium mostly uses fine chemical raw materials such as glucose and peptone as the medium raw materials. Such raw materials are expensive and are only suitable for laboratory research and cannot meet the needs of large-scale industrial production.

Method used

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Examples

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Embodiment 1

[0011] A method for making a culture medium suitable for liquid fermentation of Antrodia antrodia, which is composed of white granulated sugar, ferment liquid filter slurry, soybean protein powder, potassium dihydrogen phosphate, magnesium sulfate, vitamin B1, and agar, and the mass content percentage of each component is: white granulated sugar 4%-8%, enzyme liquid filter slurry 12%-15%, soybean protein powder 4%-8%, potassium dihydrogen phosphate 0.1%-0.5%, magnesium sulfate 0.1%-0.5%, vitamin B10.01%-0.05 %, agar 1%-2%.

[0012] The production method of the culture medium: filter the enzyme solution to get the filtrate; add soybean protein powder, white sugar or golden sugar, agar, potassium dihydrogen phosphate, magnesium sulfate, vitamin B1 to the filtrate according to the ratio, and cook on low heat until the agar dissolves , set the volume to 1000mL; divide the prepared medium into test tubes while hot, 10mL each, plug the mouth of the test tube tightly with a cotton pl...

Embodiment 2

[0016] A method for making a culture medium suitable for liquid fermentation of Antrodia camphorata, which consists of golden granulated sugar, enzyme liquid filter pulp, enzyme powder leftovers, corn steep liquor, potassium dihydrogen phosphate, magnesium sulfate, vitamin B1, and agar, and the mass content of each component The percentages are: golden granulated sugar 4%-8%, enzyme liquid filter slurry 8%-10%, enzyme powder leftovers 3%-5%, corn steep liquor 3%-5%, potassium dihydrogen phosphate 0.1%-0.5%, sulfuric acid Magnesium 0.1%-0.5%, vitamin B10.01%-0.05%, agar 1%-2%.

[0017] The production method of the culture medium: filter the enzyme solution to get the filtrate; add soybean protein powder, white sugar or golden sugar, agar, potassium dihydrogen phosphate, magnesium sulfate, vitamin B1 to the filtrate according to the ratio, and cook on low heat until the agar dissolves , set the volume to 1000mL; divide the prepared medium into test tubes while hot, 10mL each, pl...

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Abstract

Provided is a method for preparing a culture medium for Antrodia cinnamomea liquid fermentation. The culture medium comprises white granulated sugar, enzyme liquid filter pulp, soyabean protein powder, monopotassium phosphate, magnesium sulfate, vitamin B1 and agar, or comprises brown sugar, the enzyme liquid filter pulp, enzyme powder residues, corn steep liquor, the monopotassium phosphate, the magnesium sulfate, the vitamin B1 and the agar.

Description

technical field [0001] The invention relates to a preparation method of a culture medium suitable for liquid fermentation of Antrodia camphorata. Background technique [0002] Antrodia camphorata, also known as camphor mushroom and Antrodia cinnamomea, is a medicinal filamentous fungus that grows on the rotten inner wall of the camphor tree in Taiwan. Studies have shown that Antrodia camphorata has good biological activities such as anti-tumor, liver protection, anti-inflammation, and immune regulation. Due to the extremely low natural yield of Antrodia camphorata and the artificial cultivation technology of fruiting bodies has not yet been industrialized, the fruiting bodies of Antrodia camphorata are very expensive in recent years. Therefore, it is the main method to obtain the fermentation products of Antrodia camphorata by liquid culture with biotechnology methods. [0003] At present, the Antrodia camphorata liquid fermentation medium mostly uses fine chemical raw mater...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00
Inventor 许安邦
Owner JINYES QINGDAO BIOTECH CO LTD
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