Antrodia cinnamomea growth medium and preparation method and application thereof

A growth medium, the technology of Antrodia cintrodia, applied in the field of bioengineering, can solve the problems of high cost, long culture period, and low yield of mycelia biomass triterpenes, and achieve preservation, high biomass, and intracellular triterpene yields high effect

Active Publication Date: 2014-03-05
ZHEJIANG UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using these methods to cultivate Antrodia Cinnamomea not only has high cost and long culture period, but also produces low mycelial biomass and intracellular triterpenes.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Cut 200g of potatoes into thin strips, add 700ml of water and boil for 20min, filter through gauze to remove residue, add 200ml of 2% camphor leaves boiling liquid, 15g of glucose, 3g of sodium chloride, 0.01g of camphor oil, and make up to 1000ml of water, Adjust the pH to 6.5, pack 120ml of culture medium into 300ml Erlenmeyer flasks, bandage the mouth of the bottle and sterilize with high-pressure steam at 121°C for 20 minutes, take it out after the sterilization is completed, and put it aside after cooling to obtain the Antrodia camphorata growth medium . The 2% camphor leaf boiling liquid is obtained by boiling camphor leaves and water at a weight ratio of 2:98 for 10 to 15 minutes.

[0019] Use this medium to cultivate Antrodia cinnamomea mycelium: Inoculate 1-2cm-sized Antrodia cinnamomea strains into the above-mentioned medium, cultivate at 150r / min, 26°C for 5 days until a large amount of mycelium is produced, and take out the mycelium after the cultivation is ...

Embodiment 2

[0023] Cut 150g of potatoes into thin strips, add 700ml of water to boil for 15min, filter through gauze to remove residue, add 100ml of 2% camphor leaves boiling liquid, 12g of glucose, 1g of sodium chloride, 0.005g of camphor oil, and make up water to 1000ml. Adjust the pH to 7.0, divide into 300ml Erlenmeyer flasks according to 120ml of medium in each bottle, wrap the mouth of the bottle, and then sterilize with high-pressure steam at 121°C for 20 minutes. After the sterilization is completed, take it out, cool it and place it for later use, and then you can get Antrodia antrodia growth medium . The 2% camphor leaf boiling liquid is obtained by boiling camphor leaves and water at a weight ratio of 2:98 for 10 to 15 minutes.

[0024] Use this medium to cultivate Antrodia cinnamomea mycelium: Inoculate 1-2cm-sized Antrodia cinnamomea strains into the above-mentioned medium, and cultivate at 100r / min at 25°C for 6 days until a large amount of mycelium is produced, and take out...

Embodiment 3

[0028] Cut 250g of potatoes into thin strips, add 700ml of water and boil for 25min, filter through gauze to remove residue, add 250ml of 2% camphor leaves boiling liquid, 18g of glucose, 5g of sodium chloride, 0.03g of camphor oil, and add water to 1000ml, Adjust the pH to 6.8, pack 120ml of culture medium into 300ml Erlenmeyer flasks, bandage the mouth of the bottle and sterilize with high pressure steam at 121°C for 20 minutes, take it out after the sterilization is completed, and put it aside after cooling to obtain the Antrodia camphorata growth medium . The 2% camphor leaf boiling liquid is obtained by boiling camphor leaves and water at a weight ratio of 2:98 for 10 to 15 minutes.

[0029] Use this medium to cultivate Antrodia cinnamomea mycelia: Inoculate 1-2cm-sized Antrodia cinnamomea strains into the above-mentioned medium, and cultivate at a speed of 150r / min at 27°C for 3 days until a large amount of mycelium is produced, and take out the mycelium after the cultiv...

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Abstract

The invention discloses an antrodia cinnamomea growth medium and a preparation method and application thereof, belonging to the field of bioengineering technology. The antrodia cinnamomea growth medium consists of the following raw materials: 100-250ml of 2% boiled liquid of cinnamomum camphora leaves, 150-250g of potato, 12-18g of glucose, 1-5g of sodium chloride, 0.005-0.03g of cinnamomum camphora oil and water with the rest volume of 1,000ml; the pH is 6.5-7.0. Compared with the prior art, the antrodia cinnamomea growth medium disclosed by the invention has the beneficial effects that (1) the medium is simple to prepare and convenient to store, the adopted technology is mature, and the medium contains all nutritional ingredients required for the antrodia cinnamomea growth and is suitable for the culture and large-scale industrial fermentation production of antrodia cinnamomea; (2) the cultured antrodia cinnamomea mycelia have high biomass and high intracellular triterpene output, wherein the biomass of mycelia reaches 12.1-14.2g/1,000ml, and the intracellular triterpene output reaches 33.32-46.54mg/g.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a growth medium of Antrodia camphorata and its preparation method and application. Background technique [0002] Antrodia camphorata, also known as Antrodia cinnamomea, Antrodia cinnamomea, and red camphor mushroom, is a fungus of the genus Polyporaceae. It is a rare medicinal fungus native to Taiwan Province of my country. It is mainly distributed in Taoyuan and Miaoli in Taiwan Province of my country. , Nantou, Kaohsiung, and Pingtung in deep mountains and dense forests above 1000 m above sea level, saprophytic in the decayed hollow interior of Taiwan's protected tree species Cinnamomum camphora or on the wet surface of fallen trunks, the growth is extremely slow, and the natural yield is scarce. In recent years, due to the miraculous curative effect of Antrodia camphorata on liver diseases and its anti-tumor, anti-virus, anti-oxidation, anti-inflammation, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G1/00
Inventor 刘士旺张蕾毛建卫尤玉如沙如意韩金龙符丹妮黄俊吴元锋
Owner ZHEJIANG UNIVERSITY OF SCIENCE AND TECHNOLOGY
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