The invention discloses a thermosensitive
microcarrier as well as a preparation technology and an application method thereof. The thermosensitive
microcarrier can be used for amplifying, culturing and harvesting high-quality and high-activity cells used for
clinical treatment, and the high-quality and high-activity cells are applied to the fields of
cell therapy and
tissue engineering. The preparation technology comprises the following steps of:
grafting active groups by virtue of chemical surface treatment while a
high polymer material
cell culture microballoon sphere as a matrix, then carrying out reaction on the
high polymer material
cell culture microballoon sphere and
thermosensitive polymer to graft the
thermosensitive polymer, so as to obtain a thermosensitive
microcarrier used for
cell culture, and controlling the temperature during
cell culture, so that cells grow and are amplified while being attached onto the surface of the thermosensitive microcarrier; then lowering temperature and haversting cells with complete
surface protein. On one hand, a method for amplifying massive cells used for treatment in a short time, on the other hand, no pancreatin or traditional dissociative
reagent is used, so that damage on
surface protein and
ion channel of cells is reduced, the original function of the cells is maintained, and the thermosensitive microcarrier has strong clinical application value and economic value.