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Bent-branch algae culturing solution and preparation method thereof

A culture method and culture solution technology are applied in the field of Campylobacter culture solution and preparation thereof, can solve problems such as harsh environmental requirements, and achieve the effects of low cost, easy implementation and simple preparation process

Inactive Publication Date: 2011-09-28
TAIYUAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The growth of Curvola is harsh on the environment and puts forward high requirements on the culture medium. At present, there is no public report on the research on its culture medium.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Add MgSO4 7H2O34mg, CaCl236mg, KNO30.30mg, NaHCO316mg, KH2PO40.40mg, FeCl with a mass concentration of 44% in 100ml of water 3 0.05ml, add appropriate amount of NaHCO 3 Adjust the pH to 7.90.

[0019] Put 0.5 g (wet weight) of Curvola sp. into the above-mentioned culture solution at a temperature of 19.0° C., light for 12 days / 12 nights, and an intensity of 2500 LX, and add 2 g of glucose. (Wet weight refers to the weight of the algae taken from the water and placed on filter paper for 10 minutes)

[0020] According to experiments, the average weight of bentclad algae reaches 0.50g, 0.58g, 0.65g, 0.74g after being cultured in the above culture medium for 0, 3, 6, and 9 days, and the weight increases by 48% after 9 days.

Embodiment 2

[0022] The preparation method of Curvolae culture solution: add MgSO to 1000ml water 4 ·7H 2 O345.53mg, CaCl 2 368.96mg, KNO 3 3.12 mg, NaHCO 3 164.71mg, KH 2 PO 4 4.17 mg FeCl with a mass concentration of 44% 3 0.05ml, add appropriate amount of NaHCO 3 Adjust the pH to 7.95.

[0023] The cultivation method of Curvolae is that 1 g (wet weight) of Bentula is attached to the non-woven substrate, put into the above-mentioned culture solution, the temperature is 19.2° C., the light is 12 days / 12 nights, the intensity is 3000 LX, and glucose 20 g is added.

[0024] According to experiments, the average weight of bentclad algae reached 1.00 g, 1.18 g, 1.38 g, and 1.49 g after being cultured in the above-mentioned culture solution for 0, 3, 6, and 9 days, and the weight increased by 49% after 9 days.

Embodiment 3

[0026] Add MgSO to 1000ml water 4 ·7H 2 O 350mg, CaCl 2 370mg, KNO 3 4.0mg, NaHCO 3 170mg, KH 2 PO 4 5.0mg FeCl with a mass concentration of 44% 3 0.05ml, add appropriate amount of NaHCO 3 Adjust the pH to 8.00.

[0027] Attach Bentcladon algae to the stone substrate, put it into the above-mentioned culture solution at a temperature of 20.0°C, light for 12 days / 12 nights, with an intensity of 4500 LX, and add 20 g of glucose.

[0028] According to experiments, the average weight of bentclad algae reached 2.00 g, 2.38 g, 2.78 g, and 2.94 g after being cultured in the above-mentioned culture solution for 0, 3, 6, and 9 days, and the weight increased by 47% after 9 days.

[0029] After testing and comparison, it can be seen that every 1000ml of water contains MgSO 4 ·7H 2 O 340-350mg, CaCl 2 360-370mg, KNO 3 3.0-4.0mg, NaHCO 3 160-170mg, KH 2 PO 4 4.0-5.0mg FeCl with a mass concentration of 44% 3 With a ratio of 0.05ml, a better cultivation effect can be achieved,...

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Abstract

The invention relates to a bent-branch algae culturing solution and a preparation method thereof, wherein each 1000 milliliter of water contains 340 to 350 milligrams of MgSO4.7H2O, 360 to 370 milligrams of CaCl2, 3.0 to 4.0 milligrams of KNO3, 160 to 170 milligrams of MaHCO3, 4.0 to 4.5 milligrams of KH2PO4 and 0.05 milliliter of FeCl with the mass concentration of 44 percent; 20 grams of glucose is used; and NaHCO3 is used for adjusting the pH value to be 7.9 to 8.0. The culturing condition is as follows: the temperature is 18 to 20 DEG C, the bent-branch algae is illuminated for 12 days and 12 nights, and the intensity is 2500 to 4500 LX. The bent-branch algae culturing solution disclosed by the invention can promote the bent-branch algae to grow, has a simple preparation process, is convenient to implement, has a low cost, and is applicable to conservation and large-scale culturing of bent-branch algae.

Description

technical field [0001] The invention relates to an algae culture solution, in particular to a curvy algae culture solution and a preparation method thereof. Background technique [0002] The characteristics of Compsopogon coeruleus: the algal body is tufted, up to 50cm long, thicker, dark olive green, and grows on other objects with a thin base. Lateral branches finely hairy, young part smooth, up to 190-450um in diameter, with dense cortex, composed of 2-3 layers of cells, the transverse wall of the axial cells in the older part is sometimes slightly constricted; the upper part of the branch often has several Alternate, double-branched branchlets; the old part of the branch is similar in shape and structure to the main branch, the young cortex cells are slightly quadrangular, horizontal, and then gradually become polygonal, irregularly arranged, 15-36um long, diameter 10-17um, algae cells often extend downward to form thinner colorless rhizoids. Axial cells subglobose or ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G33/00
CPCY02A40/80
Inventor 石瑛张猛谢树莲李砧陈燕飞王茜
Owner TAIYUAN NORMAL UNIV
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