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Method for increasing yield of antrodia cinnamomea and bioactive substance content of antrodia cinnamomea

A bioactive substance, Antrodia camphorata technology, applied in the directions of botanical equipment and methods, plant cultivation, bulk chemical production, etc., can solve the problems of long cultivation time, high cost, and difficulty in obtaining Antrodia camphorata basswood, etc., to slow down the growth rate. and the formation of biologically active substances, the effect of increasing yield and accelerating growth rate

Active Publication Date: 2015-08-26
福州惠生食用菌有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fruiting bodies of Antrodia camphorata obtained by this method contain similar components to those of wild Antrodia camphorata. However, the cost of this cultivation method is high and the cultivation time is long, which is not conducive to mass production and promotion.
Especially the camphor tree basswood is not easy to obtain, which makes the prices of Antrodia camphorata-related products remain high

Method used

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  • Method for increasing yield of antrodia cinnamomea and bioactive substance content of antrodia cinnamomea
  • Method for increasing yield of antrodia cinnamomea and bioactive substance content of antrodia cinnamomea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] A method for increasing the yield of Antrodia camphorata and the content of biologically active substances thereof, comprising the following steps:

[0045] 1) Prepare the five-leaved pine extract:

[0046] The roots, stems, and leaves of Pinus chinensis are crushed to a particle size of 35-60 μm at a low temperature of 5°C under vacuum conditions, mixed evenly, dissolved in 20% alcohol, and subjected to ultrasonic crushing to destroy the cell walls and dissolve the resin of Pinus pinus vinifera. The ultrasonic crushing conditions are temperature 25°C, time 25min; after ultrasonic crushing, supercritical fluid extraction is performed to extract pine resin. Obtain the five leaf pine extracts with different pH;

[0047] Wherein, when the ratio of root:stem:leaf is 93:6:1, a five-leaf pine extract with pH=5.5 is obtained;

[0048] When the ratio of root:stem:leaf is 50:35:15, a five-leaf pine extract with pH=6.0 is obtained;

[0049] When the ratio of root:stem:leaf is 40...

Embodiment 2

[0065] A method for increasing the yield of Antrodia camphorata and the content of biologically active substances thereof, comprising the following steps:

[0066] 1) Prepare the five-leaved pine extract:

[0067] The roots, stems and leaves of Pinus chinensis are crushed at a low temperature of 10°C under vacuum conditions to a particle size of 35-60 μm, mixed evenly, dissolved in 20% alcohol for ultrasonic crushing, and the cell walls are destroyed to dissolve the resin of the five-leaved pine. The ultrasonic crushing conditions are temperature 30°C, time 20min; after ultrasonic crushing, supercritical fluid extraction is performed to extract pine resin. Obtain the five leaf pine extracts with different pH;

[0068] Wherein, when the ratio of root:stem:leaf is 95:4:1, a five-leaf pine extract with pH=5.5 is obtained;

[0069] When the ratio of root:stem:leaf is 45:40:15, a five-leaf pine extract with pH=6.0 is obtained;

[0070] When the ratio of root:stem:leaf is 38:32:3...

Embodiment 3

[0085] A method for increasing the yield of Antrodia camphorata and the content of biologically active substances thereof, comprising the following steps:

[0086] 1) Prepare the five-leaved pine extract:

[0087] The roots, stems and leaves of Pinus chinensis are crushed at a low temperature of 8°C under vacuum conditions to a particle size of 35-60 μm, mixed evenly, dissolved in 20% alcohol for ultrasonic crushing, and the cell walls are destroyed to dissolve the resin of the five-leaved pine. The ultrasonic crushing condition is temperature 28°C, time 20min; after ultrasonic crushing, supercritical fluid extraction is performed to extract pine resin. Obtain the five leaf pine extracts with different pH;

[0088] Wherein, when the ratio of root:stem:leaf is 95:4:1, a five-leaf pine extract with pH=5.5 is obtained;

[0089] When the ratio of root:stem:leaf is 50:35:15, a five-leaf pine extract with pH=6.0 is obtained;

[0090] When the ratio of root:stem:leaf is 40:30:30, ...

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Abstract

The invention discloses a method for increasing the yield of antrodia cinnamomea and the bioactive substance content of the antrodia cinnamomea. Roots, stems and leaves of pinus pentaphylla are mixed according to different proportions and crushed through ultrasonic waves, pinus pentaphylla rosin is extracted through supercritical fluid extraction to obtain pinus pentaphylla extracting solutions with different pH values in the growth process of the antrodia cinnamomea, a cereal culture medium is adopted, the pH value of the growing environment of antrodia cinnamomea fungi is controlled to be 5-7 by adding the pinus pentaphylla extracting solutions with the corresponding pH values, the concentration of carbon dioxide is controlled to be 1-5%, and the oxygen concentration is controlled to be 5-15%. According to the method, the growth rate of the antrodia cinnamomea can be increased, and meanwhile the content of bioactive substances such as triterpenes, polysaccharide and adenosine is increased.

Description

technical field [0001] The invention relates to a cultivation method of Antrodia camphorata, in particular to a method for increasing the yield of Antrodia camphorata and the content of biologically active substances. Background technique [0002] Cinnamomum camphorata is a unique plant in Taiwan, which is highly valued and listed as a conservation plant. Antrodia cinnamomea only grows on cinnamomum cinnamomea, and other trees cannot grow cinnamomea cinnamomea. Antrodia cinnamomea is effective in inhibiting cancer, preventing cancer metastasis, hepatitis B, diabetes, It has effects on diseases such as high blood pressure, and also has considerable effects on anti-oxidation, anti-coagulation in blood, and gastrointestinal diseases. The composition of Antrodia camphorata is complex, and the important ingredients include: triterpenoids, superoxide dismutase, adenosine, β-D-glucan and other nutritional ingredients; among them, triterpenoids are one of the most important chemical...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C05F15/00
CPCA01G18/00C05F11/00C05F11/10Y02P20/54
Inventor 林志陈玉舜
Owner 福州惠生食用菌有限公司
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