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Chosenia stem section tissue culture and rapid propagation method

A technology of tissue culture, fast propagation and willow drilling, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as low operability and repeatability, general growth conditions, etc., to improve the efficiency of breeding seedlings Effect

Active Publication Date: 2015-02-04
INST OF FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the problems of normal growth, poor operability and low repeatability in the existing tissue culture methods of willow willow, the present invention aims to provide a method for tissue culture and rapid propagation of willow willow stem segments

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Screening medium for inducing clustered buds

[0032] WPM, DKW, 2 / 3DKW and 1 / 2NRM were used as the basic medium respectively to screen out the suitable basic medium for the induction culture of the stem segment of the willow plant. Use the above-mentioned 4 kinds of basal medium to prepare medium with the same hormone types and concentrations. The hormones used are 6-BA (6-benzylaminopurine, benzylaminopurine) with a final concentration of 1.0 mg / L plus NAA with a final concentration of 0.1 mg / L. (Naphthaleneacetic acid, 1-Naphthaleneacetic acid), namely prepare the following medium (pH value is 5.80):

[0033] 1. WPM+6-BA1.0mg / L+NAA0.1mg / L medium

[0034] 2. DKW+6-BA1.0mg / L+NAA0.1mg / L medium

[0035] 3. 2 / 3DKW+6-BA1.0mg / L+NAA0.1mg / L medium

[0036] 4. 1 / 2NRM+6-BA1.0mg / L+NAA0.1mg / L medium

[0037] The stem section (3cm long, with an axillary bud) of the current year was induced to grow buds. The culture conditions were 10h-12h of light per day, and the tem...

Embodiment 2

[0041] Embodiment 2 screening dedifferentiation medium

[0042] Using WPM, DKW, 2 / 3DKW and 1 / 2NRM as the basic medium respectively, the basic medium suitable for the dedifferentiation culture of the stem segment of Zhuantian willow was screened. Prepare medium with the same types of supplements and the same concentration as above-mentioned 4 basic mediums, the supplements used are 6-BA with a final concentration of 2.0 mg / L, 0.1 mg / L NAA, 4.5 g / L agar and 20 g / L glucose , that is to prepare the following medium (pH value is 5.80):

[0043] 1. WPM+6-BA 2.0mg / L+NAA 0.1mg / L+agar 4.5g / L+glucose 20g / L medium

[0044] 2. DKW+6-BA 2.0mg / L+NAA 0.1mg / L+agar 4.5g / L+glucose 20g / L medium

[0045] 3. 2 / 3DKW+6-BA 2.0mg / L+NAA 0.1mg / L+agar 4.5g / L+glucose 20g / L medium

[0046]4. 1 / 2NRM+6-BA 2.0mg / L+NAA 0.1mg / L+agar 4.5g / L+glucose 20g / L medium

[0047] The clustered shoots obtained in Example 1 were inoculated on the above four mediums for dedifferentiation culture, the culture conditions w...

Embodiment 3

[0051] Embodiment 3 Carry out the rapid propagation of the tissue culture of willow stem section by the method of the present invention

[0052] On April 20, 2014, in Huanren County, Benxi City, the current year's branches of Zantian willow were collected, and the diameter of the branches was required to be more than 3mm. There is an axillary bud growing, and the tissue culture and rapid propagation of the stem of Zhuantian willow begins:

[0053] (the pH value of the shoot-inducing medium used below, the dedifferentiation medium, the differentiation medium, and the rooting medium are all 5.80.)

[0054] 1) Disinfection of explants: Disinfect the harvested stem segments with 0.3% by mass mercuric chloride solution for 7 minutes.

[0055] 2) cluster bud induction culture: inoculate 100 stem segments after disinfection on the cluster bud induction medium, the cluster bud induction medium is based on DKW medium as the basic medium, containing 1.0mg / L (final concentration, The s...

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Abstract

The invention belongs to the field of plant tissue culture, and discloses a chosenia stem section tissue culture and rapid propagation method which comprises the following steps: collecting chosenia one-year stem sections for successive 1) sterilization of explants, 2) induction culture of cluster buds, 3) proliferation culture of the cluster buds, 4), rooting culture of the cluster buds and 5) acclimatization and transplant to obtain chosenia tissue culture seedling. The method breaks through the bottleneck that chosenia only can breed by seeds and the survival rate is low, can help to break the chosenia endangered statu, and forms chosenia mass rapid reproduction situation. At the same time, by the use of tissue culture and rapid propagation technology, culture conditions are controllable, anniversary test or production are feasible, growth is fast, cycle is short, repeatability is strong, under breeding conditions in factories, millions of strains of finished and virus-free seedlings can be bred by each chosenia strain in one year, and chosenia seedling breeding efficiency can be greatly improved.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a method for tissue culture and rapid propagation of willow stem segments. Background technique [0002] Chosenia arbutifolia (Chosenia arbutifolia), also known as Korean willow and red-tipped willow, is a large deciduous tree in the family Salicaceae, which belongs to a single genus and a single species. This species is now on the verge of extinction. It is an endangered tree species at the Vulnerable (V) level, and it is a national second-level key protected wild plant. Zantian willow is mainly distributed in northeast Asia, starting from the Arctic Circle in the north, passing through East Siberia in the south, and entering my country. It is distributed in eastern Russia and its islands, northeastern China, northern to central Japan, and northern North Korea. Due to the deterioration of the ecological environment, some areas have disappeared, and some areas ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 季萍倩孙振元李振坚韩蕾刘俊祥王正超
Owner INST OF FORESTRY CHINESE ACAD OF FORESTRY
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