Method for culturing sugar-free tissue of jewel orchid

Abstract

The invention relates to a method for culturing sugar-free tissue of jewel orchid, which comprises the steps of: inoculating the small-segment tissue of the little sterile jewel orchid to a sugar-free induction culture medium, placing the sugar-free induction culture medium in a culture box with a top opening for carrying out tissue induction and culturing cluster buds, wherein the sugar-free induction culture medium comprises an MS culture medium, a porous inorganic material (4-10g / L), alpha-naphthylacetic acid (0.1-3.0mg / L) and 6-benzyladenine (2-4mg / L), and the pH value of the sugar-free induction culture medium is 5-6; and planting the induced cluster buds into a sugar-free strong stock culture medium, placing the sugar-free strong stock culture medium in the culture box with the top opening for culturing strong stocks, wherein the sugar-free strong stock culture medium comprises a B5 culture medium, a porous inorganic material (2-10g / L), peptone (1-5g / L), Hyponex 1 (0.1-3.0g / L), banana paste (20-150g / L) and active carbon (0.1-3g / L), and the pH value of the sugar-free strong stock culture medium is 5.5-6.0. The invention can improve the root-taking rate and the planting percent of the jewel orchid plant, and reduce the production and tissue culture cost.

Description

technical field [0001] The invention relates to a sugar-free tissue culture method of clematis clematis. Background technique [0002] Anoectochilus roxburghii (wall.) lindl is a perennial herb of the family Orchidaceae. It is also known as golden silkworm, golden thread orchid, golden thread orchid, etc. It is an endangered and rare medicinal plant, known as "golden clematis". "Grass", "magic medicine", "black ginseng" and other laudatory titles have a wide range of folk applications, and are widely used in the treatment of rheumatoid arthritis, hypertension, diabetes, kidney disease and other difficult and miscellaneous diseases, as well as for strengthening the body and post-illness. In terms of recovery from physical weakness, it is regarded as a rare and precious medicinal material in Zhejiang, Fujian, Taiwan and other provinces and Southeast Asia, especially in Taiwan Province, and is known as the "king of medicines". [0003] The seeds of clematis are tiny, consistin...

AI Technical Summary

Problems solved by technology

Under heterotrophic and multitrophic conditions, small plants mainly rely on sugar in the medium as a carbon source, which can easily cause microbial contamination;

[0006] (2) Due to the presence of sugar in the medium, the surface structure of the leaves will be poorly developed, the stomatal opening and closing functions will not be strong, the leaves will be small, and the chlorophyll content will be reduced, which will eventually inhibit and reduce the photosynthetic ability of the small plants;

[0007] (3) The cost of traditional group cultivation of seedlings is high, and it takes at least 4 months for group culture to grow into strong seedlings with a height of 7 cm, and the survival rate of plants is low

[0009] (5) The cost of traditional clematis tissue culture technology is high, and the cost of sugar accounts for 12%-15% of the cost of the medium (based on the addition of 30g of sugar per bottle of medium)