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Culture medium of rapid propagation and tissue culture of Prunus serrulata Lindl. and method of rapid propagation and tissue culture

A technology for rapid propagation of tissue culture and red-leaf cherry blossoms, which is applied in horticultural methods, botanical equipment and methods, cultivation, etc., can solve the problems of small multiplication coefficient, slow multiplication speed, and long seedling growth period, and achieve the improvement of multiplication coefficient and multiplication Short period, good cultivation effect

Inactive Publication Date: 2009-11-25
HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the tissue culture rapid propagation medium and tissue culture rapid propagation method of other woody plants are used for tissue culture rapid propagation of cherry blossoms, the tissue culture seedlings will not stretch, deform, present rosettes, and the internodes will be short. Slow multiplication rate, small multiplication coefficient, long seedling cycle and poor seedling quality

Method used

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  • Culture medium of rapid propagation and tissue culture of Prunus serrulata Lindl. and method of rapid propagation and tissue culture
  • Culture medium of rapid propagation and tissue culture of Prunus serrulata Lindl. and method of rapid propagation and tissue culture
  • Culture medium of rapid propagation and tissue culture of Prunus serrulata Lindl. and method of rapid propagation and tissue culture

Examples

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Effect test

Embodiment 1

[0030] The Red Leaf Cherry Blossom Tissue Culture Rapid Propagation Medium is prepared by dissolving the following substances in distilled water according to the type and quality of each liter.

[0031] Ammonium nitrate 500 mg, potassium sulfate 520 mg, potassium dihydrogen phosphate 150 mg, magnesium sulfate heptahydrate 350 mg, calcium nitrate tetrahydrate 750 mg, calcium chloride dihydrate 90 mg, manganese sulfate tetrahydrate 22.3 mg, zinc sulfate 8.6 mg , boric acid 6.2 mg, sodium molybdate dihydrate 0.25 mg, potassium iodide 0.83 mg, cobalt chloride hexahydrate 0.025 mg, copper sulfate pentahydrate 0.025 mg, ferrous sulfate heptahydrate 28 mg, sodium edetate dihydrate 38 mg , inositol 100 mg, niacin 0.5 mg, pyridoxine hydrochloride 0.5 mg, thiamine hydrochloride 0.1 mg, glycine 2 mg, 6-benzyl adenine 0.5 mg, sucrose 30 g, agar 6 g. The pH of the medium was adjusted to 5.6 with 1M sodium hydroxide.

[0032] Tissue culture and rapid propagation of red leaf cherry blossoms...

Embodiment 2

[0041] The preparation of red leaf cherry tissue culture rapid propagation medium: each liter contains 550 mg of ammonium nitrate, 570 mg of potassium sulfate, 170 mg of potassium dihydrogen phosphate, 400 mg of magnesium sulfate heptahydrate, 800 mg of calcium nitrate tetrahydrate, dihydrate chloride 100 mg of calcium, 56 mg of ferrous sulfate heptahydrate, 76 mg of sodium edetate dihydrate, 1.0 mg of 6-benzyl adenine; other ingredients and concentrations are the same as in Example 1; the pH of the culture medium is adjusted to 5.8. The cultivation method is the same as in Example 1 except that the dark treatment time is 7 days, and the survival rate is 90%.

Embodiment 3

[0043] The preparation of red leaf cherry tissue culture rapid propagation medium: each liter contains 525 mg of ammonium nitrate, 545 mg of potassium sulfate, 160 mg of potassium dihydrogen phosphate, 375 mg of magnesium sulfate heptahydrate, 775 mg of calcium nitrate tetrahydrate, dihydrate chloride 95 milligrams of calcium, 42 milligrams of ferrous sulfate heptahydrate, 57 milligrams of sodium edetate dihydrate, and 0.8 milligrams of 6-benzyl adenine; other ingredients and concentrations are the same as in Example 1; the pH of the medium is adjusted to 5.7.

[0044] The cultivation method is the same as in Example 1 except that the mass ratio of peat:perlite is 4:1, and the survival rate is 87%.

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Abstract

The invention belongs to the field of rapid propagation and tissue culture method of plant, more particularly relates to a culture medium of Prunus serrulata Lindl. and a method of rapid propagation and tissue culture. The culture medium consists of a large number of elements, micro elements, ferric salt, organic components, sycrose, agar and a plant growth regulating agent, a large number of elements thereof comprise: ammonium nitrate, potassium sulfate, monopotassium phosphate, magnesium sulfate heptahydrate, calcium nitrate tetrahydrate and calcium chloride dehydrate; the ferric salt thereof comprises: ferrous sulfate heptahydrate and dihydrate sodium ethylene diamine tetracetate; the plant growth regulating agent is 6-benzyladenine and has concentration of 0.5-1.0 milligram / liter; other components are the same as MS culture medium. The pH value of the culture medium is 5.6-5.8. The culture medium of rapid propagation and tissue culture and the method of rapid propagation and tissue culture, provided by the invention, are simple, convenient and easily feasible aiming at characteristics of Prunus serrulata Lindl.. The tissue culture seedling is free from malformation, elongated in internode, enhanced in propagation coefficient, short in propagation period, which is about 50 days, good in culture effect and high in survival rate.

Description

(1) Technical field [0001] The invention belongs to the field of tissue culture rapid propagation method of plants, in particular to a culture medium and tissue culture rapid propagation method of red leaf cherry blossoms. (2) Background technology [0002] Cherry blossoms, Rosaceae, Prunus, are famous ornamental flowers and trees in China. There are extensive traditional planting habits in North China, East China, South China, Central Plains and most parts of Northeast and Northwest. Many cities have designated it as "city tree city". flower". Red leaf cherry blossom is a variety of magnificent cherry blossom. After years of breeding in foreign countries, its traits have been stabilized. Its flowers are very large, light red, double-petaled, and have long stems. It blooms from mid-to-late March to early-to-mid April in Henan. Flowers open first and leaves or flowers and leaves together, the flowers are large and showy. The leaves are dark red in early spring, and bright r...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00
Inventor 孟月娥李艳敏王利民王慧娟赵秀山董晓宇
Owner HENAN ACAD OF AGRI SCI
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