Callus culture method of snow lotus herb

A technology of callus and culture method, which is applied in the field of Tianshan Snow Lotus callus culture, can solve the problems of slow growth of wild Tianshan Snow Lotus, endangered wild Tianshan Snow Lotus, low seed reproduction rate, etc., and achieves extremely market competitiveness, cycle cycle Short, non-polluting effect on the environment
CN101773071AInactive Publication Date: 2010-07-14烟台汇鹏生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
烟台汇鹏生物科技有限公司
Publication Date
2010-07-14
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a callus culture method of snow lotus herb, comprising the steps of selection and asepsis of plants, induction and culture of callus and large-scale culture. The core technology of the method is selection of the following large-scale culture medium: MS+0-50g / L of cane sugar+0-10g / L of agar+0.1-5.0mg / L of alpha-naphthylacetic acid+0.1-5.0mg / L of 6-benzyladenine. During large-scale culture, an abiotic elicitor, 40-60mu mol / L of SNP+40-60mu mol / L of MJ+40-60mu mol / L of SA is added. The method is easy to operate, and the active ingredient is high in content and low in production cost, does not pollute environment and can reach the level of industrialization. Syringin produced by the culture technology in the invention is invariant, high in content, low in cost and short in cycle and is very competitive in the markets.
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Description

technical field

[0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for culturing callus of Snow Lotus in Tianshan Mountains. Background technique

[0002] Tianshan Snow Lotus (Saussurea involucrate Kar.et Kir.) belongs to Angiosperm, Dicotyledoneae, Compositae, Asteraceae, Artichoke family, and Phyllostachys genus. As early as the eighth century, there were records of snow lotus in the ancient Tibetan medical literature "Yuewang Yaozheng", and later in bibliographies such as "Northwest Territory Records", "Jingzhu Materia Medica", "Lan Liuli", "Compendium of Materia Medica Supplements" and other bibliographies. recorded. It is mainly distributed in the alpine regions such as Xinjiang, Qinghai, Gansu, Yunnan and Tibet in my country, between rock crevices and cliffs near the snow line at an altitude of 2400-4100m, and rarely distributed abroad. Snow lotus is warm in nature and slightly bitter in taste. It enters...

Claims

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