A tissue culture method suitable for the seedlings of Chinese herbal medicine Thyme chinensis
A technology of tissue culture and Chinese herbal medicine, applied in the field of plant propagation, can solve the problems of high-efficiency, rapid and large-scale industrial planting and cultivation of seedlings, and achieve the effects of accelerating the speed of popularization and application, easy operation, and strong practical significance
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0023] (1) Pretreatment: Take the healthy and full-bodied Chinese herbal medicine Thyme chinensis terminal buds or axillary buds, use a soft brush to add a small amount of detergent to the water and gently wipe the branches and axillary buds, rinse with tap water for 1 hour, and put them in an ultra- In the clean workbench, sterilize with 70% alcohol for 20 seconds, rinse with sterile water for 4 times, sterilize with mercuric chloride with a concentration of 0.1% for 8 minutes, and rinse with sterile water for 5 times; Inoculate in a test tube filled with germination medium for germination culture, inoculate one terminal bud or axillary bud in each test tube, and then place it in the culture room. The germination medium is: MS basal medium + 6-BA 2.0mg / L +NAA0.2mg / L+sucrose 30g / L+agar powder 5.4g / L, pH 5.8; cultured for 5 weeks at a temperature of 25°C, a light time of 12h / d, and a light intensity of 1500 lux, during which time the contaminated materials were removed;
[0024...
Embodiment 2
[0028] (1) Pretreatment: Take the healthy and plump Thyme Thyme seed material, remove the outer testa, sterilize with alcohol with a volume fraction concentration of 65% for 40 seconds on the ultra-clean workbench, rinse with sterile water 3 times, The mass fraction concentration is 0.2% mercuric chloride for 10 minutes, rinsed with sterile water for 3 times; the materials that have been sterilized on the surface are inoculated into culture bottles filled with germination medium for germination culture, and one seedling is inoculated in each culture bottle. Seeds are then placed in the cultivation chamber, and the germination medium is: on the germination medium of seed germination medium MS+6-BA 2.0mg / L+GA30mg / L+sucrose 20g / L+agar powder 5.4g / L, pH 7.0, Place it in the cultivation room for dark cultivation, cultivate for 5 weeks, and count the germination rate with the growth of young leaves as the germination standard;
[0029](2) Clumping bud induction culture: take the ger...
Embodiment 3
[0033] (1) Pretreatment: Take the healthy and full-bodied Chinese herbal medicine Thyme chinensis terminal buds or axillary buds, use a soft brush to add a small amount of detergent to the water, and gently wipe the branches and axillary buds, rinse with tap water for 1.5 hours, and put them in the water for 1.5 hours. In the ultra-clean workbench, sterilize with 75% alcohol for 30 seconds, wash with sterile water for 4 times, sterilize with mercuric chloride with a concentration of 0.2% for 8 minutes, and rinse with sterile water for 5 times; the surface is sterilized Germination culture is carried out by inoculating the material in a test tube filled with germination medium. Each test tube is inoculated with one terminal bud or axillary bud, and then placed in the culture room. The germination medium is: MS basal medium + 6-BA 2.0mg / L+NAA0.2mg / L+sucrose 30g / L+agar powder 5.4g / L, pH 5.8; cultured for 5 weeks at a temperature of 27°C, a light time of 16h / d, and a light intensi...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com